A5014124991- Trypanosoma species research and implications
- Viral Infections and Immunology Research
- Cardiomyopathy and Myosin Studies
- Transplantation: Methods and Outcomes
- Cardiac Structural Anomalies and Repair
- Cardiac pacing and defibrillation studies
- Cardiovascular Effects of Exercise
- Cardiac Arrhythmias and Treatments
- Eosinophilic Disorders and Syndromes
- Cardiac Valve Diseases and Treatments
- Cardiac tumors and thrombi
- Research on Leishmaniasis Studies
- Cardiovascular Function and Risk Factors
- Public Health in Brazil
- Congenital Heart Disease Studies
- Infective Endocarditis Diagnosis and Management
- Mechanical Circulatory Support Devices
- Blood Pressure and Hypertension Studies
- Synthesis and Biological Evaluation
- Pulmonary Hypertension Research and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Studies on Chitinases and Chitosanases
- Vascular anomalies and interventions
- Pericarditis and Cardiac Tamponade
- Signaling Pathways in Disease
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2010-2025
Universidade de São Paulo
2014-2024
Instituto do Coração
2003-2024
University of Pisa
2023
Hospital do Coração
2012-2019
Hospital Universitário da Universidade de São Paulo
2007-2016
Centro Universitário das Faculdades Metropolitanas Unidas
2006-2012
Massachusetts General Hospital
2011
Duke Medical Center
2011
Universidade Brasil
2008
Background Chronic Chagas cardiomyopathy (CCC), a life-threatening inflammatory dilated cardiomyopathy, affects 30% of the approximately 8 million patients infected by Trypanosoma cruzi. Even though Th1 T cell-rich myocarditis plays pivotal role in CCC pathogenesis, little is known about factors controlling cell migration to myocardium. Methods and Results Using confocal immunofluorescence quantitative PCR, we studied surface staining gene expression CXCR3, CCR4, CCR5, CCR7, CCR8 receptors...
The role of Trypanosoma cruzi in the pathogenesis myocarditis chronic phase Chagas' disease is still controversial, with autoimmune mechanisms frequently being proposed. In present work, we demonstrate that higher numbers CD8+ T cells are correlated presence parasite antigens, suggesting an important for development myocardial inflammation. Quantification mean and CD4+ per 400x microscopic field was performed specimens from 33 chagasic patients heart failures (nine biopsies 24 necropsies),...
In São Paulo State, Brazil, five males, aged between 8 and 64 years, were attacked by 'Africanized' honey bees (Apis mellifera scutellata). The estimated number of stings received each patient ranged from > 200 to 1000. All transferred intensive care units in City. Clinical features included intravascular haemolysis, respiratory distress with ARDS, hepatic dysfunction, rhabdomyolysis (with myoglobinaemia myoglobinuria), hypertension myocardial damage (perhaps explained release endogenous...
Endocardial fibrous tissue (FT) deposition is a hallmark of endomyocardial fibrosis (EMF). Echocardiography first-line and the standard technique for diagnosis this disease. Although late gadolinium enhancement (LGE) cardiovascular magnetic resonance (CMR) allows FT characterization, its role in prognosis EMF has not been investigated.Thirty-six patients (29 women; age, 54±12 years) with after clinical evaluation comprehensive 2-dimensional Doppler echocardiography underwent cine-CMR...
The present study assessed the possible involvement of renin-angiotensin system (RAS) and sympathetic nervous (SNS) in thyroxine (T 4 )-induced cardiac hypertrophy. Hemodynamic parameters, heart weight (HW), ratio HW to body (HW/BW), myocyte width were evaluated absence thyroid hormone (hypothyroidism) after T administration. Male Wistar rats used. Some subjected thyroidectomies, whereas hyperthyroidism was induced others via daily intraperitoneal injection (25 or 100 μg · g BW -1 day ) for...
Myocyte diameter, fractional area of collagen, intensity myocarditis and parasite persistence (explored by immunohistochemistry PCR) were evaluated in serial sections endomyocardial biopsies from 29 outpatients with chronic chagasic cardiopathy. The patients, 25 males four females a mean (S.D.) age 43 (9) years, subsequently followed up for 3–2861 days (median=369 days). During this follow-up, 16 (55%) the patients died.The revealed (86%) high-grade 14 (56%). Although failed to demonstrate...
Chronic Chagas disease cardiomyopathy (CCC), a late consequence of Trypanosoma cruzi infection, is an inflammatory with prognosis worse than those noninflammatory etiology (NIC). Although the T cell-rich myocarditis known to play pathogenetic role, relative contribution each functional cell subsets has never been thoroughly investigated. We therefore assessed gene expression cytokines and transcription factors involved in differentiation effector function subset (TH1/TH2/TH17/Treg) CCC, NIC,...
Long noncoding RNAs (lncRNAs) modulate gene expression at the epigenetic, transcriptional, and posttranscriptional levels. Dysregulation of lncRNA known as myocardial infarction–associated transcript (MIAT) has been associated with infarction. Chagas disease causes a severe inflammatory dilated chronic cardiomyopathy (CCC). We investigated role MIAT in CCC. A whole-transcriptome analysis heart biopsy specimens formalin-fixed, paraffin-embedded samples revealed that was overexpressed patients...
Resveratrol (RSV) improves metabolic and cardiovascular functions, but its tissue-specific effects on diabetes remain unclear. This study investigated RSV's impact cardiac skeletal muscle adipose tissue in a rat model. Thirty-two Wistar rats were assigned to four groups: control (C), treated with RSV (RC), diabetic (D), (RD). Diabetes was induced using streptozotocin nicotinamide. After two weeks, RC RD groups received for six weeks. Histological protein expression analyses...
Resveratrol (RSV) improves metabolic functions, but its tissue-specific effects on diabetes remain unclear. This study investigated RSV's impact molecular pathways in an experimental model of cardiac and skeletal muscles adipose tissue. Wistar rats were assigned to control (C), treated with RSV (RC), diabetic (D), (RD). Diabetes was induced using streptozotocin nicotinamide, administered for six weeks. In rats, treatment significantly reduced collagen accumulation muscle tissues compared...
We report a human immunodeficiency virus (HIV)-infected man with chronic Chagas' disease who developed congestive heart failure that could not be clinically controlled. Endomyocardial biopsy revealed severe myocarditis and the xenodiagnosis result was positive, but Trypanosoma cruzi by direct microscopic examination of blood found only four months after symptoms had started. Treatment benznidazole effective in reducing parasitemia, stabilizing clinical status, controlling tissue damage...
Background Chronic Chagas disease cardiomyopathy (CCC) is an inflammatory dilated with a worse prognosis than other cardiomyopathies. CCC occurs in 30 % of individuals infected Trypanosoma cruzi, endemic Latin America. Heart failure associated impaired energy metabolism, which may be correlated to contractile dysfunction. We thus analyzed the myocardial gene and protein expression, as well activity, key mitochondrial enzymes related ATP production, samples end-stage CCC, idiopathic (IDC)...
Cardiac remodeling in diabetes involves cardiac hypertrophy and fibrosis, fibroblast growth factor 2 (FGF2) is an important mediator of this process. Resveratrol, a polyphenolic antioxidant, reportedly promotes the improvement dysfunction diabetic rats. However, little information exists linking amelioration function promoted by resveratrol expression FGF2 its co-receptors, heparan sulfate proteoglycans (HSPGs: Glypican-1 Syndecan-4), muscle Type Diabetes was induced experimentally injection...
Chronic Chagas disease (CCC) is an inflammatory dilated cardiomyopathy with a worse prognosis compared to other cardiomyopathies. We show the expression and activity of Matrix Metalloproteinases (MMP) their inhibitors TIMP (tissue inhibitor metalloproteinases) in myocardial samples end stage CCC, idiopathic (DCM) patients, from organ donors. Our results showed significantly increased mRNA several MMPs, TIMPs EMMPRIN CCC DCM samples. MMP-2 TIMP-2 protein levels were elevated both sample...
Cardiac interstitial fibrosis may contribute to ventricular dysfunction and the prognosis of patients with dilated cardiomyopathy. The objective present study was determine if total myocardial collagen content type III/I (III/I ratio) mRNAs differ in hypertensive, alcoholic, idiopathic cardiomyopathy subjects. Echocardiography exercise cardiopulmonary testing were performed (N = 22), hypertensive 12), alcoholic 11) Morphometric analysis fragments obtained by endomyocardial biopsy picrosirius...
Chagas disease cardiomyopathy (CCC) is an inflammatory dilated occurring in 30% of the 6 million infected with protozoan Trypanosoma cruzi Latin America. Survival significantly lower CCC than ischemic (IC) and idiopathic (DCM). Previous studies disclosed a selective decrease mitochondrial ATP synthase alpha expression creatine kinase activity myocardium as compared to IDC IC, well decreased vivo myocardial production. Aiming identify additional constraints energy metabolism specific CCC, we...
A variety of signaling pathways are involved in the induction innate cytokines and CD8+ T cells, which major players protection against acute Trypanosoma cruzi infection. Previous data have demonstrated that a TBK-1/IRF3-dependent pathway promotes IFN-β production response to cruzi, but role for STING, main interactor these proteins, remained be addressed. Here, we STING is required IFN-β, IL-6, IL-12 infection absence negatively impacts activation IRF-dependent parasite. We reported no...
Pain is the most conspicuous symptom observed in patients wounded by stingrays, and skin necrosis common accidents freshwater stingrays. The extract from stinger integumentary tissue of Potamotrygon falkneri containing toxic components (venom) was tested for its ability to induce histopathological changes dorsal mice at different times. 3-6 h after injection, foci isolated basal epidermal cells were observed. Full coagulative skin, subcutaneous skeletal muscle evident as soon 24 venom...