A5066675650- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Biosensors and Analytical Detection
- Insect symbiosis and bacterial influences
- Dengue and Mosquito Control Research
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- Viral Infections and Outbreaks Research
- Vector-Borne Animal Diseases
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Vector-borne infectious diseases
- vaccines and immunoinformatics approaches
Fundação Oswaldo Cruz
2016-2024
Zika virus (ZIKV) is a flavivirus that has recently been associated with an increased incidence of neonatal microcephaly and other neurological disorders. The primarily transmitted by mosquito bite, although routes infection have implicated in some cases. Aedes aegypti considered to be the main vector humans worldwide; however, there evidence species, including Culex quinquefasciatus, transmit virus. To test potential Cx. quinquefasciatus ZIKV, we experimentally compared competence...
Abstract In low-resource settings, resilience to infectious disease outbreaks can be hindered by limited access diagnostic tests. Here we report the results of double-blinded studies performance paper-based tests for Zika and chikungunya viruses in a field setting Latin America. The involved cell-free expression system relying on isothermal amplification toehold-switch reactions, purpose-built portable reader onboard software computer vision-enabled image analysis. patients suspected...
Abstract The rapid spread of Zika virus (ZIKV) represents a global public health problem, especially in areas that harbor several mosquito species responsible for transmission, such as Brazil. In these areas, improvement control needs to be top priority, but viral surveillance occurs inefficiently ZIKV-endemic countries. Quantitative reverse transcription PCR (qRT-PCR) is the gold standard molecular diagnostic ZIKV both human and samples. However, technique presents high cost limitations...
Multiple epicenters of the SARS-CoV-2 pandemic have emerged since first pneumonia cases in Wuhan, China, such as Italy, USA, and Brazil. Brazil is third-most affected country worldwide, but genomic sequences strains are mostly restricted to states from Southeast region. Pernambuco state, located Northeast region, sixth most Brazilian very few circulating this region available. We sequenced 101 patients presenting Covid-19 symptoms that reside Pernambuco. Phylogenetic reconstructions revealed...
Mayaro virus (MAYV) is transmitted by Haemagogus spp. mosquitoes and has been circulating in Amazon areas the North Central West regions of Brazil since 1980s, with an increase human case notifications last 10 years. MAYV introduction urban a public health concern as infections can cause severe symptoms similar to other alphaviruses. Studies Aedes aegypti have demonstrated potential vector competence species detection populations mosquitoes. Considering two most abundant mosquito Brazil, we...
Zika virus (ZIKV) has been isolated from many mosquito species in nature, but it is believed that the main vectors urban environments are of genus Aedes. Here, we detected and ZIKV samples Aedes aegypti, taeniorhynchus Culex quinquefasciatus, collected during epidemic Vitória, southeast Brazil. Using quantitative real-time polymerase chain reaction, detection was performed February to April 2016. Overall, six pools mosquitoes were positive for ZIKV: four Cx. one Ae. aegypti taeniorhynchus....
Abstract Zika virus (ZIKV) is a flavivirus that has recently been associated with increased incidence of neonatal microcephaly and other neurological disorders. The primarily transmitted by mosquito bite, although routes infection have implicated in some cases. Aedes aegypti considered to be the main vector humans worldwide, but there evidence species, including Culex quinquefasciatus , playing role Brazilian outbreak. To test this hypothesis, we experimentally compared vectorial competence...
The (re) emergence of arboviruses around the world is a public health concern once severe outbreaks are usually associated with these infections. co-circulation Dengue (DENV), Zika (ZIKV) and Chikungunya (CHIKV) viruses in past few years has caused unique epidemic situation Brazil. northeast region country was most affected by clinical complications from such arboviruses’ infections, including neurological disorders ZIKV. In this particular region, Aedes mosquitoes main vectors DENV, ZIKV...
<p>In low-resource settings, resilience to infectious disease outbreaks can be hindered by limited access diagnostic tests. Here we report the results of double-blinded studies performance paper-based tests for Zika and chikungunya viruses in a field setting Latin America. The involved cell-free expression system relying on isothermal amplification toehold-switch reactions, purpose-built portable reader onboard software computer vision-enabled image analysis. In patients suspected...
The co-occurrence of different arboviruses, through (re)emergence episodes in a particular region, often exacerbates public health crises associated with these pathogens. Considering the Brazilian epidemiological panorama arboviruses cocirculation, such as Dengue (DENV), Zika (ZIKV), and Chikungunya (CHIKV) viruses, imminent emergence Mayaro virus (MAYV) urban areas, presence susceptible human hosts, we looked into coinfection superinfection impact CHIKV MAYV replication Aedes aegypti....
Since 2015, the Dengue, Zika, and Chikungunya viruses gained notoriety for their impact in public health many parts of globe, including Brazil. In Recife, capital Pernambuco State, introduction ZIKV impacted human population tremendously, owing to increase number neurological cases, such as Guillain−Barré congenital Zika disorders. Later, Recife was considered be epicenter epidemics For arboviral diseases, there are some risk factors, climate changes, low socioeconomic conditions, high...
ABSTRACT The emergence of SARS-CoV-2 in the human population has caused a huge pandemic that is still unfolding many countries around world. Multiple epicenters have emerged since first pneumonia cases Wuhan, Italy followed by USA and Brazil. Up to now, Brazil second most affected country, however, genomic sequences strains circulating country are restricted some highly impacted states. Although Pernambuco state, located Northeast Region, sixth brazilian state considering lethality rate,...
Abstract Mayaro virus (MAYV) is transmitted by Haemagogus spp . mosquitoes and has been circulating in Amazon areas the North Central West regions of Brazil since 1980s, with an increase human case notifications last 10 years. MAYV introduction urban a public health concern once infection can cause severe symptoms similar to other Alphaviruses. Regarding transmission, studies Aedes aegypti demonstrate potential vector competence species detection populations mosquitoes. Considering two most...
Introduction: Zika virus (ZIKV) has emerged as a major global public health concern in the last five years due to its link causative agent of congenital malformations thousands newborns.Currently, reverse transcriptase reaction followed by quantitative polymerase chain (RT-qPCR) is considered reference method diagnose ZIKV-infection.Nevertheless, RT-qPCR requires technical expertise and utilizes specialized equipment for amplification detection viral genome.These drawbacks negatively impact...
<p>In low-resource settings, resilience to infectious disease outbreaks can be hindered by limited access diagnostic tests. Here we report the results of double-blinded studies performance paper-based tests for Zika and chikungunya viruses in a field setting Latin America. The involved cell-free expression system relying on isothermal amplification toehold-switch reactions, purpose-built portable reader onboard software computer vision-enabled image analysis. In patients suspected...
Introduction:The rapid spread of Zika virus (ZIKV) represents a global public health problem, especially in countries that circulate several vector mosquito vectors and favorable conditions for transmission, such as Brazil.In these areas, improvement control needs to be top priority, but viral surveillance occurs inefficiently endemic countries.Currently, the reverse transcriptase reaction followed by quantitative polymerase chain (qRT-PCR) is gold standard molecular diagnostic ZIKV...