A5066124345- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Antibiotic Resistance in Bacteria
- Insect Resistance and Genetics
- Poxvirus research and outbreaks
- Vector-borne infectious diseases
- Bacillus and Francisella bacterial research
- Bioinformatics and Genomic Networks
- Herpesvirus Infections and Treatments
- Malaria Research and Control
- vaccines and immunoinformatics approaches
- Vibrio bacteria research studies
- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Bacterial biofilms and quorum sensing
- Biochemical and Molecular Research
- Yersinia bacterium, plague, ectoparasites research
- Entomopathogenic Microorganisms in Pest Control
- Aquaculture disease management and microbiota
- Enterobacteriaceae and Cronobacter Research
- Salmonella and Campylobacter epidemiology
- Insect and Pesticide Research
Fundação Oswaldo Cruz
2016-2025
Instituut voor Tropische Geneeskunde
2022-2024
Research Institute for Tropical Medicine
2023
Universidade Federal de Pernambuco
2021
University of Georgia
2020
Companhia Energética de Minas Gerais (Brazil)
2014
Universidade Federal de Minas Gerais
2012
Hospital Municipal Souza Aguiar
2002
The magnitude of the 2022 multi-country monkeypox virus (MPXV) outbreak has surpassed any preceding outbreak. It is unclear whether asymptomatic or otherwise undiagnosed infections are fuelling this epidemic. In study, we aimed to assess occurred among men attending a Belgian sexual health clinic in May 2022. We retrospectively screened 224 samples collected for gonorrhea and chlamydia testing using an MPXV PCR assay identified MPXV-DNA-positive from four men. At time sampling, one man had...
Zika virus (ZIKV) infection during pregnancy is associated with microcephaly, a congenital malformation resulting from neuroinflammation and direct effects of replication on the developing central nervous system (CNS). However, exact changes in affected CNS remain unknown. Here, we show by transcriptome analysis (at 48 hours post-infection) multiplex immune profiling that human induced-neuroprogenitor stem cells (hiNPCs) respond to ZIKV strong induction type I interferons (IFNs) several IFNs...
Abstract Background Monkeypox is transmitted by close contact with symptomatic cases, and those infected are assumed to be uniformly symptomatic. Evidence of subclinical monkeypox infection limited a few immunological studies which found evidence immunity against orthopoxviruses in asymptomatic individuals who were exposed cases. We aimed assess whether infections occurred among underwent sexually (STI) screening large Belgian STI clinic around the start 2022 epidemic Belgium. Methods...
Multiple epicenters of the SARS-CoV-2 pandemic have emerged since first pneumonia cases in Wuhan, China, such as Italy, USA, and Brazil. Brazil is third-most affected country worldwide, but genomic sequences strains are mostly restricted to states from Southeast region. Pernambuco state, located Northeast region, sixth most Brazilian very few circulating this region available. We sequenced 101 patients presenting Covid-19 symptoms that reside Pernambuco. Phylogenetic reconstructions revealed...
Mpox (formerly monkeypox) is a viral disease caused by the mpox virus (MPXV), endemic in Central and West Africa currently causing global outbreak of international concern. Much remains unknown about sample types most suited for laboratory diagnosis. While it established that high loads can be found active skin lesions (currently recommended confirmation specimen type), WHO testing guidelines encourage use oropharyngeal swabs as an additional type diagnosis suggest investigating value other...
The rapid worldwide spread of chikungunya (CHIKV), dengue (DENV), and Zika (ZIKV) viruses have raised great international concern. Knowledge about the entry routes geographic expansion these arboviruses to mainland Americas remain incomplete controversial. Epidemics caused by continue cause socioeconomic burden globally, particularly in countries where vector control is difficult due climatic or infrastructure factors. Understanding how virus circulates moves from one country another...
Acinetobacter baumannii is an opportunistic bacterial pathogen infecting immunocompromised patients and has gained attention worldwide due to its increased antimicrobial resistance. Here, we report a comparative whole-genome sequencing analysis coupled with assessment of antibiotic resistance 46 strains (45 A. plus one nosocomialis) originated from five hospitals the city Recife, Brazil, between 2010 2014. An average 3,809 genes were identified per genome, although only 2,006 single copy...
Aeromonas spp. are gram-negative bacteria that can cause a variety of infections in both humans and animals play controversial role diarrhea outbreaks. Our aim was to identify clinical environmental isolates associated with cholera outbreak northeast county Brazil at the species level. We also aimed determine genetic structure bacterial population virulence potential isolates.Analysis based on concatenated sequences 16S rRNA gyrB genes suggested classification 119 studied into following...
The leishmaniasis are neglected tropical diseases widespread throughout the globe which caused by protozoans from genus Leishmania and transmitted infected phlebotomine flies. development of a safe effective vaccine against these has been seen as best alternative to control reduce number cases. To support development, this work applied an in silico approach search for high potential peptide epitopes able bind different Major Histocompatibility Complex Class I II (MHC MHC II) molecules human...
Background: Endogenous viral elements (EVEs) are sequences of origin integrated into the host genome. EVEs have been characterized in various insect genomes, including mosquitoes. A large EVE content has found Aedes aegypti and albopictus genomes among which a recently described Chuviridae family is particular interest, owing to abundance derived from it, discrepancy chuvirus endogenized gene regions frequent association with retrotransposons BEL-Pao superfamily. In order better understand...
Abstract A large proportion of HIV-coinfected visceral leishmaniasis (VL-HIV) patients exhibit chronic disease with frequent VL recurrence. However, knowledge on immunological determinants underlying the course is scarce. We longitudinally profiled circulatory cellular immunity an Ethiopian HIV cohort that included developers. show VL-HIV high and persistent levels TIGIT PD-1 CD8 + /CD8 - T cells, in addition to a lower frequency IFN-γ suggestive impaired cell functionality. At single...
The initiation of translation in eukaryotes is supported by the action several eukaryotic Initiation Factors (eIFs). largest these eIF3, comprising up to thirteen polypeptides (eIF3a through eIF3m), involved multiple stages process. eIF3 has been better characterized from model organisms, but poorly known more diverged groups, including unicellular lineages represented human pathogens. These include trypanosomatids (Trypanosoma and Leishmania) other protists belonging taxonomic supergroup...
Plasmodium vivax infection is characterized by a dormant hepatic stage, the hypnozoite that activated at varying periods of time after clearance primary acute blood-stage, resulting in relapse. Differentiation between treatment failure and new infections requires characterization initial infections, relapses, clone multiplicity malaria infections.Parasite DNA obtained from primary/relapse paired blood samples 30 patients with P. Brazil was analyzed using 10 molecular markers (8...
The Trypanosomatids parasites Leishmania braziliensis, major and infantum are important human pathogens. Despite of years study genome availability, effective vaccine has not been developed yet, the chemotherapy is highly toxic. Therefore, it clear just interdisciplinary integrated studies will have success in trying to search new targets for developing vaccines drugs. An essential part this rationale related protein-protein interaction network (PPI) which can provide a better understanding...
Desulfovibrio gigas is a model organism of sulfate-reducing bacteria which energy metabolism and stress response have been extensively studied. The complete genomic context this was however, not yet available. sequencing the D. genome provides insights into integrated network conserving complexes structures present in bacterium. Comparison with genomes other spp. reveals presence two different CRISPR/Cas systems gigas. Phylogenetic analysis using conserved protein sequences (encoded by rpoB...
Plague is a deadly zoonosis that still poses threat in many regions of the world. We combined epidemiologic, host, and vector surveillance data collected during 1961-1980 from Araripe Plateau focus northeastern Brazil with ecologic, geoclimatic, Yersinia pestis genomic information to elucidate how these factors interplay plague activity. identified well-delimited hotspots showing elevated risk low-altitude areas near foothills plateau's concave sectors. Those locations exhibited distinct...
To describe the genetic diversity of Plasmodium vivax isolates from different areas in Brazilian Amazon using 11 polymorphic microsatellites and to evaluate correlation between microsatellite variation repeat array length.Microsatellites with variable units lengths were selected silico search P. genome. We designed primers amplified loci DNA obtained patients acute infections.Positive length was detected independently size unit (di, tri, or tetranucleotide). used these markers variability...
The potential use of CRISPR loci genotyping to elucidate population dynamics and microevolution 146 Yersinia pestis strains from different biovars locations was investigated in this work. majority the Orientalis biovar presented specific spacer arrays, allowing for establishment a signature their respective isolates. Twenty-one new spacers were found Y. plague foci Brazil. Ninety-three (64%) grouped G1 genotype, whereas others distributed 35 genotypes. This study allowed observing...
Vaccination is important in containing the 2022 mpox (formerly monkeypox) epidemic. We describe five Belgian patients with localised severe symptoms of proctitis and penile oedema, occurring between 4 35 days after post-exposure preventive vaccination or one- two-dose off-label pre-exposure MVA-BN vaccine. Genome sequencing did not reveal evidence for immune escape variants. Healthcare workers those at risk should be aware possible infections shortly need other measures.
Abstract Background Epitope prediction using computational methods represents one of the most promising approaches to vaccine development. Reduction time, cost, and availability completely sequenced genomes are key points highly motivating regarding use reverse vaccinology. Parasites genus Leishmania widely spread they etiologic agents leishmaniasis. Currently, there is no efficient against this pathogen drug treatment toxic. The lack sufficiently large datasets experimentally validated...
The leishmaniasis are parasitic diseases caused by protozoans of the genus Leishmania, highly divergent eukaryotes, characterized unique biological features. To survive in both mammalian hosts and insect vectors, these pathogens make use a number mechanisms, many which associated with parasite specific proteases. metalloprotease GP63, major Leishmania surface antigen, has been found to have multiple functions required for parasite's survival. GP63 is encoded genes their copy numbers vary...