A5038788635- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Parasites and Host Interactions
- Plant Molecular Biology Research
- Evolution and Genetic Dynamics
- Plant Stress Responses and Tolerance
- Plant Reproductive Biology
- Plant Parasitism and Resistance
- Plant and animal studies
- Plant responses to elevated CO2
- Biochemical and Molecular Research
- Photosynthetic Processes and Mechanisms
- Insect and Pesticide Research
- Atmospheric chemistry and aerosols
- Light effects on plants
- Immune Cell Function and Interaction
- Chromosomal and Genetic Variations
- Plant nutrient uptake and metabolism
- Immunodeficiency and Autoimmune Disorders
- Genomics and Chromatin Dynamics
- Synthesis and Biological Evaluation
- Circadian rhythm and melatonin
- CRISPR and Genetic Engineering
- Polysaccharides and Plant Cell Walls
- Renal Diseases and Glomerulopathies
Instituut voor Tropische Geneeskunde
2016-2025
Universidad Nacional de Córdoba
2024
Tribhuvan University
2024
B.P. Koirala Institute of Health Sciences
2024
Beltsville Agricultural Research Center
2024
Agricultural Research Service
2024
Max Planck Institute for Plant Breeding Research
2004-2023
Research Institute for Tropical Medicine
2016-2023
Museum of Arts and Design
2021
University of Antwerp
2013-2019
The biosynthesis of the recently identified novel class plant hormones, strigolactones, is up-regulated upon phosphate deficiency in many species. It generally accepted that evolutionary origin strigolactone up-regulation their function as a rhizosphere signal stimulates hyphal branching arbuscular mycorrhizal fungi. In this work, we demonstrate induction conserved Arabidopsis (Arabidopsis thaliana), although not host for We increase production contributes to changes shoot architecture...
Strigolactones (SLs), or their derivatives, were recently demonstrated to act as endogenous shoot branching inhibitors, but biosynthesis and mechanism of action are poorly understood. Here we show that the phenotype mutants in Arabidopsis P450 family member, MAX1, can be fully rescued by strigolactone addition, suggesting MAX1 acts SL synthesis. We demonstrate SLs modulate polar auxin transport control both synthetic GR24 synthesis significantly reduce basipetal a second branch-regulating...
ABSTRACT Aneuploidy is usually deleterious in multicellular organisms but appears to be tolerated and potentially beneficial unicellular organisms, including pathogens. Leishmania , a major protozoan parasite, emerging as new model for aneuploidy, since vitro -cultivated strains are highly aneuploid, with interstrain diversity intrastrain mosaicism. The alternation of two life stages different environments (extracellular promastigotes intracellular amastigotes) offers unique opportunity...
Circadian systems coordinate endogenous events with external signals. In mammals, hormone‐clock feedbacks are a well‐known integration system. Here, we investigated phytohormone effects on plant‐circadian rhythms via the promoter:luciferase We report that many hormones control specific features of system, and do so in distinct ways. particular, cytokinins delay circadian phase, auxins regulate amplitude clock precision, brassinosteroid abscisic acid modulate periodicity. confirmed...
Abstract Climate changes increasingly threaten plant growth and productivity. Such are complex involve multiple environmental factors, including rising CO 2 levels climate extreme events. As the molecular physiological mechanisms underlying responses to realistic future conditions still poorly understood, a organizational level analysis (i.e. eco ‐ physiological, biochemical, transcriptional) was performed, using Arabidopsis exposed incremental heat wave water deficit under ambient elevated...
A main developmental switch in the life cycle of a flowering plant is transition from vegetative to reproductive growth. In Arabidopsis thaliana, distinct genetic pathways regulate timing this transition. We report here that brassinosteroid (BR) signaling establishes an unexpected and previously unidentified pathway floral-regulating network. isolated two alleles brassinosteroid-insensitive 1 (bri1) as enhancers late-flowering autonomous-pathway mutant luminidependens(ld). bri1 was found...
Leishmania donovani causes visceral leishmaniasis (VL), the second most deadly vector-borne parasitic disease. A recent epidemic in Indian subcontinent (ISC) caused up to 80% of global VL and over 30,000 deaths per year. Resistance against antimonial drugs has probably been a contributing factor persistence this epidemic. Here we use whole genome sequences from 204 clinical isolates track evolution epidemiology L. ISC. We identify independent radiations that have emerged since bottleneck...
Background Genetic interactions between phytohormones in the control of flowering time Arabidopsis thaliana have not been extensively studied. Three individually connected to floral-timing program. The inductive function gibberellins (GAs) is most documented. Abscisic acid (ABA) has demonstrated delay flowering. Finally, promotive role brassinosteroids (BRs) established. It reported that for many physiological processes, hormone pathways interact ensure an appropriate biological response....
Summary Proline (Pro) is a versatile metabolite playing role in the protection of plants against environmental stresses. To gain deeper understanding regulation Pro metabolism under predicted future climate conditions, including drought stress, elevated temperature and CO 2 , we combined measurements contrasting grassland species (two grasses two legumes) at multiple organisational levels, that is, concentrations, enzyme activities gene expression. Drought stress (D) activates biosynthesis...
Antimonials (Sb) were used for decades chemotherapy of visceral leishmaniasis (VL). Now abandoned in the Indian subcontinent (ISC) because Leishmania donovani resistance, this drug offers a unique model understanding resistance dynamics. In previous phylogenomic study, we found two distinct populations L. donovani: core group (CG) Gangetic plains and ISC1 Nepalese highlands. Sb was only encountered within CG, series potential markers identified. Here, analyzed development to trivalent...
Whole genome sequencing (WGS) is increasingly used for molecular diagnosis and epidemiology of infectious diseases. Current Leishmania genomic studies rely on DNA extracted from cultured parasites, which might introduce sampling biological biases into the subsequent analyses. Up to now, direct analysis in clinical samples hampered by high levels human large variation parasite load samples. Here, we present a method, based target enrichment donovani with Agilent SureSelect technology, that...
Leishmania, a unicellular eukaryotic parasite, is unique model for aneuploidy and cellular heterogeneity, along with their potential role in adaptation to environmental stresses. Somy variation within clonal populations was previously explored small subset of chromosomes using fluorescence hybridization methods. This phenomenon, termed mosaic (MA), might have important evolutionary functional implications but remains under-explored due technological limitations. Here, we applied validated...
Significance Chromosome and gene copy number variations often correlate with the evolution of microbial cancer drug resistance, thus causing important human mortality. How genome instability is harnessed to generate beneficial phenotypes how deleterious dosage effects are compensated remain open questions. The protist pathogen Leishmania exploits regulate expression via changes. Using these parasites as a unique model system, we uncover complex epistatic interactions between compensatory...
Leishmania aethiopica is a zoonotic Old World parasite transmitted by Phlebotomine sand flies and causing cutaneous leishmaniasis in Ethiopia Kenya. Despite range of clinical manifestations high prevalence treatment failure, L. one the most neglected species genus terms scientific attention. Here, we explored genome diversity analyzing genomes twenty isolates from Ethiopia. Phylogenomic analyses identified two strains as interspecific hybrids involving parent donovani tropica respectively...
Aneuploidy is generally considered harmful, but in some microorganisms, it can act as an adaptive mechanism against environmental stress. Here, we use Leishmania-a protozoan parasite with remarkable genome plasticity-to study the early steps of aneuploidy evolution under high drug pressure (using antimony or miltefosine stressors). By combining single-cell genomics, lineage tracing cellular barcodes, and longitudinal characterization, reveal that changes result from polyclonal selection...
Abstract A large proportion of HIV-coinfected visceral leishmaniasis (VL-HIV) patients exhibit chronic disease with frequent VL recurrence. However, knowledge on immunological determinants underlying the course is scarce. We longitudinally profiled circulatory cellular immunity an Ethiopian HIV cohort that included developers. show VL-HIV high and persistent levels TIGIT PD-1 CD8 + /CD8 - T cells, in addition to a lower frequency IFN-γ suggestive impaired cell functionality. At single...
Abstract Background Plant circadian systems regulate various biological processes in harmony with daily environmental changes. In Arabidopsis thaliana , the underlying clock mechanism is comprised of multiple integrated transcriptional feedbacks, which collectively lead to global patterns rhythmic gene expression. The networks are essential within itself and its output pathway. Results Here, expand understanding associated clock, we performed both an silico analysis transcript rhythmicity...
We sequenced Leishmania donovani genomes in blood samples collected emerging foci of visceral leishmaniasis western Nepal. detected lineages very different from the preelimination main parasite population, including a new lineage and rare one previously reported eastern Our findings underscore need for genomic surveillance.
Isoprene emissions from poplar (Populus spp.) plantations can influence atmospheric chemistry and regional climate. These respond strongly to temperature, [CO2], drought, but the superimposed effect of these three climate change factors are, for most part, unknown. Performing predicted scenario simulations (periodic chronic heat drought spells [HDSs] applied under elevated [CO2]), we analyzed volatile organic compound emissions, photosynthetic performance, leaf growth, overall carbon (C)...
Leishmania donovani is the responsible agent for visceral leishmaniasis (VL) in Indian subcontinent (ISC). The disease lethal without treatment and causes 0.2 to 0.4 million cases each year. Recently, reports of VL Nepalese hilly districts have increased as well caused by L. from ISC1 genetic group, a new emerging genotype. In this study, we perform first time an integrated, untargeted genomics metabolomics approach characterize ISC1, comparison with Core Group (CG), main population that...
Throughout the temperate zones, plants face combined drought and heat spells in increasing frequency intensity. Here, we compared periodic (intermittent, i.e., high-frequency) versus chronic (continuous, high-intensity) drought-heat stress scenarios gray poplar (Populus× canescens) for phenotypic transcriptomic effects during after recovery. Photosynthetic productivity recovery exceeded performance of trees without experience. We analyzed molecular basis this stress-related memory phenotype...
Abstract Leishmaniasis is caused by parasitic protozoa of the genus Leishmania and found widely across tropics sub-tropics, afflicting hundreds thousands people. The disease notoriously difficult to treat. Here, we present evidence existence persister-like cells in cultured populations, induced upon exposure normally lethal doses antimony, a used anti-leishmanial. Persisters are small fraction non-proliferative with reduced metabolism that adapted withstand variety environmental assaults,...