A5016912925- Kawasaki Disease and Coronary Complications
- Pain Mechanisms and Treatments
- Inflammasome and immune disorders
- Pharmacological Effects of Natural Compounds
- IL-33, ST2, and ILC Pathways
- Exercise and Physiological Responses
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Bioactive Compounds and Antitumor Agents
- Dermatology and Skin Diseases
- Vasculitis and related conditions
- Coronary Artery Anomalies
- Phytochemistry and Biological Activities
- Eosinophilic Esophagitis
- Healthcare and Venom Research
- Phytochemicals and Antioxidant Activities
- Plant Toxicity and Pharmacological Properties
- Essential Oils and Antimicrobial Activity
- Cancer, Stress, Anesthesia, and Immune Response
- Abdominal vascular conditions and treatments
- Streptococcal Infections and Treatments
- Natural product bioactivities and synthesis
- Gout, Hyperuricemia, Uric Acid
- Biochemical effects in animals
- Marine Sponges and Natural Products
- Diabetic Foot Ulcer Assessment and Management
Universidade Estadual de Londrina
2015-2025
Cedars-Sinai Medical Center
2019-2025
The flavonoid vitexin (1) is a flavone C-glycoside (apigenin-8-C-β-d-glucopyranoside) present in several medicinal and other plants. Plant extracts containing 1 are reported to possess antinociceptive, anti-inflammatory, antioxidant activities. However, the only evidence that exhibits antinociceptive activity was demonstrated acetic acid-induced writhing model. Therefore, analgesic effects mechanisms of were evaluated. In investigation, intraperitoneal treatment with dose-dependently...
Vanillic acid (1) is a flavoring agent found in edible plants and fruits. It an oxidized form of vanillin. Phenolic compounds substantial part plant foods used as antioxidants with beneficial biological activities. These have received considerable attention because their role preventing human diseases. Especially, 1 presents antibacterial, antimicrobial, chemopreventive effects. However, the mechanisms by which exerts its anti-inflammatory effects vivo are incompletely understood. Thus,...
Neuropathic pain from injury to the peripheral and CNS represents a major health care issue. We have investigated role of IL-33/IL-33 receptor (ST2) signaling in experimental models neuropathic mice. Chronic constriction (CCI) sciatic nerve induced IL-33 production spinal cord. IL-33/citrine reporter mice revealed that oligodendrocytes are main cells expressing within cord together with minor expression by neurons, microglia. astrocytes. CCI-induced mechanical hyperalgesia was reduced IL-33R...
Rationale: NLRP3 (NLR family pyrin domain containing 3) activation and IL-1β (interleukin-1β) production are implicated in Kawasaki disease (KD) pathogenesis; however, a detailed complete characterization of the molecular networks cellular subsets involved development cardiovascular lesions is still lacking. Objective: Here, murine model KD vasculitis, we used single-cell RNA sequencing spatial transcriptomics to determine landscape inflamed vascular tissues. Methods Results: We observe...
Gouty arthritis is characterized by an intense inflammatory response to monosodium urate crystals (MSU), which induces severe pain and reduction in the life quality of patients. Trans-Chalcone (1,3-diphenyl-2-propen-1-one) a flavonoid precursor presenting biological activities such as anti-inflammatory antioxidant proprieties. Thus, aim this work was evaluate protective effects trans-Chalcone experimental gout mice. Mice were treated with (3, 10, or 30mg/kg, per oral) vehicle (Tween 80 20%...
It is currently accepted that superoxide anion (O2•−) an important mediator in pain and inflammation. The role of inflammation has been mainly determined indirectly by modulating its production inactivation. Direct evidence using potassium (KO2), a donor, demonstrated it induced thermal hyperalgesia, as assessed the Hargreaves method. However, remains to be whether KO2 capable inducing other inflammatory nociceptive responses attributed anion. Therefore, present study, we investigated...
Pimaradienoic acid (PA; ent-pimara-8(14),15-dien-19-oic acid) is a pimarane diterpene found in plants such as Vigueira arenaria Baker (Asteraceae) the Brazilian savannas. Although there evidence on analgesic and vitro inhibition of inflammatory signaling pathways, paw edema by PA, its anti-inflammatory effect deserves further investigation. Thus, objective present study was to investigate PA carrageenan-induced peritoneal inflammation mice. Firstly, we assessed leukocyte recruitment cavity...
Cancer pain directly affects the patient’s quality of life. We have previously demonstrated that subcutaneous administration mammary adenocarcinoma known as Ehrlich tumor induces in mice. Several studies shown flavonoid quercetin presents important biological effects, including anti-inflammatory, antioxidant, analgesic, and antitumor activity. Therefore, analgesic effect mechanisms were evaluated tumor-induced cancer Intraperitoneal (i.p.) treatments with reduced mechanical thermal...
SUMMARY Alterations in the intestinal microbiota contribute to pathogenesis of various cardiovascular disorders, but how they affect development Kawasaki disease (KD), an acute pediatric vasculitis, remains unclear. We report that depleting gut reduces inflammation a murine model mimicking KD vasculitis. The lesions was associated with alterations composition and, notably, decreased abundance Akkermansia muciniphila and Faecalibacterium prausnitzii. Oral supplementation either these live or...
BACKGROUND: Alterations in the intestinal microbiota contribute to pathogenesis of various cardiovascular disorders, but how they affect development Kawasaki disease, an acute pediatric vasculitis, remains unclear. Here, using a murine model mimicking disease we assessed contribution vascular inflammation. METHODS AND RESULTS: We report that depleting gut reduces inflammation vasculitis. The lesions was associated with alterations composition and, notably, decreased abundance Akkermansia...
The present study evaluated the anti-inflammatory and analgesic effects of superoxide dismutase mimetic agent tempol in anion-induced pain inflammation. Mice were treated intraperitoneally with (10-100 mg/kg) 40 min before intraplantar injection a anion donor, potassium (KO2, 30 μg). Mechanical hyperalgesia thermal hyperalgesia, paw edema, mRNA expression peripheral spinal cord mediators involved inflammatory pain, TNFα, IL-1β, IL-10, COX-2, preproET-1, gp91phox, Nrf2, GFAP, Iba-1,...
Kawasaki disease (KD), an acute febrile illness and systemic vasculitis, is the leading cause of acquired heart in children industrialized countries. KD leads to development coronary artery aneurysms (CAA) affected children, which may persist for months even years after phase disease. There unmet need characterize immune pathological mechanisms long-term complications KD. We examined cardiovascular Lactobacillus casei cell wall extract (LCWE) mouse model KD-like vasculitis over 4 months. The...
Unaccustomed exercise involving eccentric contractions, high intensity, or long duration are recognized to induce delayed-onset muscle soreness (DOMS). Myocyte damage and inflammation in affected peripheral tissues contribute sensitize nociceptors leading pain. However, despite the essential role of spinal cord regulation pain, neuroinflammatory mechanisms intense swimming-induced DOMS remain be investigated. We hypothesized that neuroinflammation contributes DOMS. C57BL/6 mice swam for 2 h...
Granulocyte-colony stimulating factor (G-CSF) is a current pharmacological approach to increase peripheral neutrophil counts after anti-tumor therapies. Pain most relevant side effect of G-CSF in healthy volunteers and cancer patients. Therefore, the mechanisms G-CSF-induced hyperalgesia were investigated focusing on role spinal mitogen-activated protein (MAP) kinases ERK (extracellular signal-regulated kinase), JNK (Jun N-terminal Kinase) p38, PI(3)K (phosphatidylinositol 3-kinase). induced...
Granulocyte-colony stimulating factor (G-CSF) is a therapeutic approach to increase peripheral neutrophil counts after anti-tumor therapies. Pain the major side effect of G-CSF. Intraplantar administration G-CSF in mice induces mechanical hyperalgesia. However, mechanisms involved this were not elucidated. Therefore, participation pronociceptive cytokines tumor necrosis (TNF) alpha (TNFα), interleukin (IL)-1 beta (IL-1β) and antinociceptive cytokine IL-10 G-CSF-induced hyperalgesia was...
Monosodium urate crystals (MSU) deposition induces articular inflammation known as gout. This disease is characterized by intense and pain mechanisms involving the activation of transcription factor NFκB inflammasome resulting in production cytokines oxidative stress. Despite evidence that MSU iNOS expression, there no on effect nitric oxide (NO) donors Thus, present study evaluated ruthenium complex donor NO {[Ru(bpy)2(NO)SO3](PF6)} (complex I) gout arthritis. Complex I inhibited a...
Pimaradienoic acid (1) is a pimarane diterpene (ent-pimara-8(14),15-dien-19-oic acid) extracted at high amounts from various plants including Vigueira arenaria Baker. Compound 1 inhibited carrageenan-induced paw edema and acetic acid-induced abdominal writhing, which are its only known anti-inflammatory activities. Therefore, it important to further investigate the analgesic effects of 1. Oral administration (1, 3, 10 mg/kg) writhing. This was also observed mg/kg via sc ip routes. Both...