A5101789243- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Eosinophilic Esophagitis
- Asthma and respiratory diseases
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Dermatology and Skin Diseases
- Autoimmune and Inflammatory Disorders Research
- Research on Leishmaniasis Studies
- Cytokine Signaling Pathways and Interactions
- Pediatric health and respiratory diseases
- Sirtuins and Resveratrol in Medicine
- Advanced Nanomaterials in Catalysis
- Immune cells in cancer
- Vector-Borne Animal Diseases
- Toxin Mechanisms and Immunotoxins
- Atherosclerosis and Cardiovascular Diseases
- Food Allergy and Anaphylaxis Research
- Air Quality and Health Impacts
- Climate Change and Health Impacts
- Inhalation and Respiratory Drug Delivery
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Plant-based Medicinal Research
- Computational Drug Discovery Methods
- Exercise and Physiological Responses
Shenzhen University
2019-2025
Shenzhen Third People’s Hospital
2021-2025
State Key Laboratory of Respiratory Disease
2021-2024
Shenzhen University Health Science Center
2023-2024
Shenzhen Luohu People's Hospital
2022
University of Glasgow
2006-2015
Institute of Laboratory Animal Science
2014
Chinese Academy of Medical Sciences & Peking Union Medical College
2014
Ningbo University
2012
Shantou University
2012
Abstract Alternatively activated macrophages (AAM) play a crucial role in type 2 immunity. Mice deficient ST2, receptor for the latest member of IL-1 family, IL-33, have impaired immune responses. We therefore reasoned that IL-33/ST2 signaling may be involved differentiation and activation AAM during airway inflammation. report here IL-33 changed quiescent phenotype alveolar toward an expressed mannose receptor, IL-4Rα, produced high levels CCL24 CCL17 IL-13-dependent manner IL-33-induced...
Atherosclerosis is a chronic inflammatory disease of the vasculature commonly leading to myocardial infarction and stroke. We show that IL-33, which novel IL-1–like cytokine signals via ST2, can reduce atherosclerosis development in ApoE−/− mice on high-fat diet. IL-33 ST2 are present normal atherosclerotic humans. Although control PBS-treated developed severe inflamed plaques aortic sinus, lesion was profoundly reduced IL-33–treated animals. also markedly increased levels IL-4, -5, -13, but...
Abstract Type 2 cytokines (IL-4, IL-5, and IL-13) play a pivotal role in helminthic infection allergic disorders. CD4+ T cells which produce type can be generated via IL-4-dependent -independent pathways. Although the pathway is well documented, factors that drive IL-4-independent Th2 cell differentiation remain obscure. We report here new cytokine IL-33, presence of Ag, polarizes murine human naive into population mainly IL-5 but not IL-4. This polarization requires IL-1R-related molecule...
IL-33, a cytokine of the IL-1 family, is closely associated with type II T cell responses. Here, we report an unexpected proinflammatory role IL-33 in inflammatory arthritis. was expressed synovial fibroblasts from patients rheumatoid arthritis (RA). Expression markedly elevated vitro by cytokines. Mice lacking ST2, receptor α-chain, developed attenuated collagen-induced (CIA) and reduced ex vivo collagen-specific induction cytokines (IL-17, TNFα, IFNγ), antibody production. Conversely,...
Toll-like receptors (TLRs) are primary sensors of both innate and adaptive immune systems play a pivotal role in response against structurally conserved components pathogens. Synthetic bacterial lipoprotein (BLP) Pam3Cys-SK4 is TLR2 agonist that capable modulating T cell responses. We show here BLP, together with anti-CD3 antibody [T receptor (TcR) activation], induced proliferation CD4+ CD25+ regulatory cells (Tregs) CD25- (effector) the absence antigen-presenting cells. The expanded Tregs...
Abstract IL-33 (IL-1F11) binds ST2 (IL-1R4), both of which are associated with optimal CD4+ Th2 polarization. Exogenous drives induction Th2-associated cytokines and pathological changes within the gut mucosa. polarization is also a prerequisite to expulsion intestinal-dwelling nematode Trichuris muris. In this study, we demonstrate that mRNA expressed early during parasite infection susceptible mice can be induced expel by regime exogenous administration. prevents an inappropriate...
The initiation and regulation of pulmonary fibrosis are not well understood. IL-33, an important cytokine for respiratory diseases, is overexpressed in the lungs patients with idiopathic fibrosis.We aimed to determine effects mechanism IL-33 on development severity murine bleomycin-induced fibrosis.Lung was induced by bleomycin wild-type or Il33r (St2)(-/-) C57BL/6 mice treated recombinant mature form anti-IL-33 antibody transferred type 2 innate lymphoid cells (ILC2s). evaluated based lung...
IL-33 is a novel cytokine of the IL-1 family and mediates its biological effect via receptor ST2, which selectively expressed on Th2 cells but not Th1 cells. drives production Th2-associated cytokines including IL-5 IL-13 thereby promotes defense pathology in mucosal organs. Cell locomotion crucial to induction an effective immune response. We report here chemoattraction by IL-33. Recombinant increased proportion human cells, polarized morphology vitro stimulated their subsequent invasion...
Chronic low-grade inflammation involving adipose tissue likely contributes to the metabolic consequences of obesity. The cytokine interleukin (IL)-33 and its receptor ST2 are expressed in tissue, but their role during obesity is unclear.To examine functional IL-33 tissues investigate effects on vivo.We demonstrate that treatment cultures vitro with induced production Th2 cytokines (IL-5, IL-13, IL-10) reduced expression adipogenic genes. Administration recombinant genetically obese diabetic...
Interleukin (IL)-33, a member of the IL-1 cytokine family, is an important modulator immune system associated with several immune-mediated disorders. High levels IL-33 are expressed by central nervous (CNS) suggesting potential role in autoimmune CNS diseases. We have investigated expression and function development experimental encephalomyelitis (EAE) mice. report here that its receptor ST2 (IL-33Rα) highly spinal cord tissue, markedly increased cords mice EAE. Furthermore, ST2-deficient...
Anaphylactic shock is characterized by elevated immunoglobulin-E (IgE) antibodies that signal via the high affinity Fc epsilon receptor (Fc epsilonRI) to release inflammatory mediators. Here we report novel cytokine interleukin-33 (IL-33) potently induces anaphylactic in mice and associated with symptom humans. IL-33 a new member of IL-1 family ligand for orphan ST2. In humans, levels are substantially blood atopic patients during shock, inflamed skin tissue dermatitis patients. murine...
Abstract IL-33 has emerged as an important mediator in the immunopathogenesis of allergy and asthma. However, role eosinophil-mediated inflammation not been fully explored. In this article, we report that directly stimulates eosinophil differentiation from CD117+ progenitors IL-5–dependent manner. Although resting eosinophils expressed moderate levels IL-33R α-chain (ST2L), accumulated airways mice with OVA-induced asthma increased amounts ST2L. vitro, GM-CSF are potent inducers ST2L...
ST2/ST2L, a member of the IL-1R gene family, is expressed by fibroblasts, mast cells, and Th2, but not Th1, cells. It exists in both membrane-bound (ST2L) soluble forms (ST2). Although ST2L has immunoregulatory properties, its ligand, cellular targets, mode action remain unclear. Using ST2-human IgG fusion protein, we demonstrated that ST2 bound to primary bone marrow-derived macrophages (BMM) this binding was enhanced treatment with LPS. The sST2 BMMs inhibited production LPS-induced...
<h3>Objectives</h3> Interleukin 33 (IL-33) is a new member of the IL-1 family cytokines which signals via its receptor, ST2 (IL-33R), and has an important role in Th2 mast cell responses. This study shows that IL-33 orchestrates neutrophil migration arthritis. <h3>Methods results</h3> Methylated bovine serum albumin (mBSA) challenge knee joint mBSA-immunised mice induced local accompanied by increased IL-33R mRNA expression. Cell was inhibited systemic treatments with soluble (s)IL-33R,...
IL-33, a new member of the IL-1 family, signals through its receptor ST2 and induces T helper 2 (Th2) cytokine synthesis mediates inflammatory response. We have investigated role IL-33 in antigen-induced hypernociception. Recombinant induced cutaneous articular mechanical hypernociception time- dose-dependent manner. The was inhibited by soluble (s) (a decoy IL-33), antagonist (IL-1ra), bosentan [a dual endothelin (ET)(A)/ET(B) antagonist], clazosentan (an ET(A) antagonist), or indomethacin...
Background Alcohol intake is inversely related to rheumatoid arthritis (RA) disease incidence and severity. Resveratrol, a safe, well-described plant-derived compound, possesses anti-inflammation immune-regulatory properties present in red wine. As such, it could mediate anti-inflammatory of the latter offer novel therapeutic utility own right. Objective To evaluate effect resveratrol on collagen-induced (CIA) its putative immune modulation mice. Methods CIA was induced DBA1 mice by...
Objective. IL-33 has recently been found to be the specific ligand of ST2, an IL-1 receptor family member that is selectively expressed in Th2 cells and mediates response. This study aims measure serum levels soluble ST2 (sST2) patients with SLE examine their association disease activity. Methods. Seventy were evaluated for activity, determined by SLEDAI, anti-dsDNA antibody, C3 C4. Fifty-seven longitudinally on a second occasion. sST2 measured sandwich ELISA 127 samples compared 28 age-...
Regulatory B cells (Breg) have attracted increasing attention for their roles in maintaining peripheral tolerance. Interleukin 33 (IL-33) is a recently identified IL-1 family member, which leads double-life with both pro- and anti-inflammatory properties. We report here that peritoneal injection of IL-33 exacerbated inflammatory bowel disease IL-10-deficient (IL-10(-/-)) mice, whereas IL-33-treated IL-10-sufficient (wild type) mice were protected from the induction. A phenotypically...
The regulation and function of IgE in healthy individuals antigen-naïve animals is not well understood. IL-33 administration increases serum mice with unknown mechanism. We tested the hypothesis that provides an antigen-independent stimulus for production mast cell degranulation.IL-33 was administered to naïve wild-type (WT), nude ST2(-/-) , IL-4(-/-) IL4Rα(-/-) T-or B-cell-specific IL-4Rα(-/-) mice. cytokines were quantified by ELISA. T- B-lymphocyte numbers CD40L expression determined flow...
Neuropathic pain from injury to the peripheral and CNS represents a major health care issue. We have investigated role of IL-33/IL-33 receptor (ST2) signaling in experimental models neuropathic mice. Chronic constriction (CCI) sciatic nerve induced IL-33 production spinal cord. IL-33/citrine reporter mice revealed that oligodendrocytes are main cells expressing within cord together with minor expression by neurons, microglia. astrocytes. CCI-induced mechanical hyperalgesia was reduced IL-33R...
Abstract Background Ambient fine particulate matter (PM 2.5 ) is considered a plausible contributor to the onset of chronic obstructive pulmonary disease (COPD). Mechanistic studies are needed augment causality epidemiologic findings. In this study, we aimed test hypothesis that repeated exposure diesel exhaust particles (DEP), model PM , causes COPD-like pathophysiologic alterations, consequently leading development specific phenotypes. Sprague Dawley rats, representing healthy lungs, were...
Abstract Rheumatoid arthritis is characterized by chronic inflammatory infiltration of the synovium, leading to eventual cartilage and bone destruction. Previously, we have reported that soluble T1/ST2 (sST2), a member IL-1R gene family, inhibits LPS-induced macrophage proinflammatory cytokine production. In this study, report therapeutic effect sST2-Fc in murine model collagen-induced arthritis. A short term administration fusion protein significantly attenuated disease severity compared...
Rheumatoid arthritis pathogenesis comprises dysregulation in both innate and adaptive immunity. There is therefore intense interest the factors that integrate these immunologic pathways rheumatoid arthritis. In this paper, we report IL-33, a novel member of IL-1 family, can exacerbate anti-glucose-6-phosphate isomerase autoantibody-induced (AIA). Mice lacking ST2 (ST2(-/-)), IL-33 receptor alpha-chain, developed attenuated AIA reduced expression articular proinflammatory cytokines....