A5065050286- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Immune Cell Function and Interaction
- Parasites and Host Interactions
- Autoimmune and Inflammatory Disorders Research
- T-cell and Retrovirus Studies
- Urticaria and Related Conditions
- IL-33, ST2, and ILC Pathways
- NF-κB Signaling Pathways
- Hepatitis Viruses Studies and Epidemiology
- Pediatric health and respiratory diseases
- Reproductive System and Pregnancy
- Adenosine and Purinergic Signaling
- Cytokine Signaling Pathways and Interactions
- Women's cancer prevention and management
- Asthma and respiratory diseases
- Immune Response and Inflammation
- Toxoplasma gondii Research Studies
- Health Education and Validation
- Climate Change and Health Impacts
- Birth, Development, and Health
- Public Health in Brazil
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA Interference and Gene Delivery
- Viral gastroenteritis research and epidemiology
University of Pennsylvania
2021-2024
Universidade de São Paulo
2014-2023
Philadelphia University
2023
Clinics Hospital of Ribeirão Preto
2016
Cutaneous leishmaniasis caused by Leishmania parasites exhibits a wide range of clinical manifestations. Although influence disease severity, cytolytic CD8 T cell responses mediate disease. While these originate in the lymph node, we found that expression effector molecule granzyme B was restricted to lesional cells Leishmania-infected mice, suggesting local cues within inflamed skin induced function. Expression Blimp-1 (Prdm1), transcription factor necessary for differentiation, driven...
Cytolytic CD8 + T cells mediate immunopathology in cutaneous leishmaniasis without controlling parasites. Here, we identify factors involved cell migration to the lesion that could be targeted ameliorate disease severity. CCR5 was most highly expressed chemokine receptor patient lesions, and high expression of CCL3 CCL4, ligands, associated with delayed healing lesions. To test requirement for CCR5, Leishmania- infected Rag1 -/- mice were reconstituted cells. We found these developed smaller...
The relationship established between Leishmania infantum and the vertebrate host can lead to a self-healing infection or manifestation of visceral leishmaniasis, chronic systemic associated with high rates mortality. We hypothesized that regulatory cytokines, such as interleukin-27 (IL-27), play role in susceptibility L. infection. IL-27 is heterodimeric cytokine composed IL-27p28 EBi3 subunits which, when combined, bind IL-27R, leading STAT-1 -3 activation, playing regulation immune...
<ns3:p>Background Pregnant individuals and children are particularly vulnerable to the adverse health consequences of exposure heat. Leveraging robust ecosystem Brazilian linked administrative data, we aim advance our understanding how high temperatures heatwaves influence birth outcomes child nutrition. Objectives Investigate association between ambient temperatures/ 1) (low weight (LBW), small for gestational age (SGA), large (LGA), preterm birth), 2) nutrition (growth), 3) breastfeeding...
Leishmania infantum is a protozoan parasite that causes visceral leishmaniasis (VL). This infection triggers dendritic cell (DC) activation through the recognition of microbial products by Toll-like receptors (TLRs). Among TLRs, TLR9 required for DC different species. We demonstrated upregulated in vitro and vivo during infection. show C57BL/6 mice deficient expression (TLR9(-/-) mice) are more susceptible to display higher numbers spleen liver. The increased susceptibility TLR9(-/-) was due...
Visceral leishmaniasis (VL) is a chronic and fatal disease caused by Leishmania infantum in Brazil. Leukocyte recruitment to infected tissue crucial event for the control of infections such as VL. Among inflammatory cells, neutrophils are recruited site infection, these cells may parasite replication through oxidative or non-oxidative mechanisms. The recruitment, activation functions coordinated pro-inflammatory cytokines chemokines during recognition pattern receptors (PRRs). Here, we...
To evaluate the clinical efficacy of a mixture probiotics (Lactobacillus and Bifidobacterium) in children adolescents with atopic dermatitis (AD) effects on sensitization, inflammation, immunological tolerance.In this double-blind, randomized, placebo-controlled trial, we enrolled 60 patients aged between 6 months 19 years mild, moderate, or severe AD, according to criteria proposed by Hanifin Rajka. Patients were stratified receive one gram per day placebo for months. The primary outcome...
Innate lymphoid cells (ILCs) comprise a heterogeneous population of immune that maintain barrier function and can initiate protective or pathological response upon infection. Here we show the involvement IL-17A-producing ILCs in microbiota-driven immunopathology cutaneous leishmaniasis. were RORγt+ enriched Leishmania major infected skin, topical colonization with Staphylococcus epidermidis before L. infection exacerbated skin inflammatory responses ILC accumulation without impacting type 1...
The prostaglandin, 15-deoxy<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msup><mml:mrow><mml:mi mathvariant="normal">Δ</mml:mi></mml:mrow><mml:mrow><mml:mn mathvariant="normal">12,14</mml:mn></mml:mrow></mml:msup></mml:mrow></mml:math>-prostaglandin J 2 (15d-PGJ ), is a lipid mediator that plays an important role in the control of chronic inflammatory disease. However, prostanoid rheumatoid arthritis (RA) not well determined. We demonstrated therapeutic...
Cutaneous leishmaniasis exhibits a spectrum of clinical presentations dependent upon the parasites' persistence and host immunopathologic responses. Although cytolytic CD8 T cells cannot control parasites, they significantly contribute to pathologic In murine model cutaneous leishmaniasis, we previously found that NKG2D plays role in ability promote disease leishmanial lesions. Here, investigated whether human disease. We its ligands were expressed within lesions from L....
Adenosine is an endogenously released purine nucleoside that signals through four widely expressed G protein-coupled receptors: A1, A2A, A2B, and A3. Of these, A2AR recognized as mediating major adenosine anti-inflammatory activity. During cutaneous leishmaniasis, induces immunosuppression, which promotes the establishment of infection. Herein, we demonstrated signaling exploited by Leishmania infantum parasites, etiologic agent causes Visceral Leishmaniasis, to successfully colonize...
A novel T cell-secreted cytokine, termed secreted osteoclastogenic factor of activated cells (SOFAT) that induces osteoclastic bone resorption in a RANKL-independent manner, has been described. Our group have previously reported SOFAT is highly expressed gingival tissues patients with chronic periodontitis suggesting putative role the loss associated periodontal disease. The aim present study was to identify other potential cellular sources resorptive lesions Gingival were biopsied from...
Sand fly saliva plays a crucial role in establishing Leishmania infection. We identified adenosine (ADO) and monophosphate (AMP) as active pharmacologic compounds present Phlebotomus papatasi that inhibit dendritic cell (DC) functions through PGE2/IL 10-dependent mechanism.Herein, we prepared mixture of ADO AMP equimolar amounts similar to those the salivary-gland extract (SGE) form one pair salivary glands P. co-injected it with amazonensis or L. major into mouse ears. ADO+AMP mimicked...
Protein-energy malnutrition (PEM) is a risk factor for developing visceral leishmaniasis (VL). While nutrient deficiency can impair immunity, its mechanistic impact on protective adaptive immune responses following Leishmania infection remains unknown. To determine the potential negative impacts of anti-parasitic in chronic VL, we provided mice with polynutrient-deficient diet (deficient protein, energy, zinc, and iron) that mimics moderate human malnutrition. The resulted growth stunting...
Visceral leishmaniasis (VL) is a chronic and fatal disease caused by Leishmania infantum in Brazil. Leukocyte recruitment to infected tissue crucial event for the control of infections such as VL. Leucotriens are lipid mediators synthesized 5-lipoxygenase (5-LO) they display protective role against protozoan parasites inducing several functions leucocytes. We determined 5-LO activity parasite control, focusing on inflammatory immune response infection. LTB 4 released during vitro The genetic...
A striking feature of human visceral leishmaniasis (VL) is chronic inflammation in the spleen and liver, VL patients present increased production levels multiple inflammatory mediators, which contribute to tissue damage disease severity. Here, we combined an experimental model with transcriptional profile demonstrate that TLR4-IFN-β pathway regulates process associated asymptomatic form disease. Tlr4-deficient mice harbored fewer parasites their liver than wild-type mice. TLR4 deficiency...
In vertebrate hosts, Leishmania braziliensis parasites infect mainly mononuclear phagocytic system cells, which when activated by T helper cell type 1 cytokines produce nitric oxide and kill the pathogens. Chemokine (C-C motif) receptor 2 is a chemokine that binds primarily ligand has an important role in recruitment of monocytic phagocytes. Although it been reported infection induces CCR2 expression lesions, during remains unknown. Here, we showed mediating protection against mice. The...
Protein-energy malnutrition (PEM) is a risk factor for developing visceral leishmaniasis (VL). However, the impact on adaptive immunity during infection unknown. To study effect of chronic VL, we used polynutrient-deficient diet (deficient protein, energy, zinc, and iron), which mimics moderate human malnutrition, followed by
Monocytes play key roles in the maintenance of homeostasis and control infection. are recruited from bone marrow to inflammatory sites essential for antimicrobial activity limit tissue damage promote adaptive T cell responses. Here, we investigated role NFAT1 regulation Ly6Chi monocyte recruitment CNS upon T. gondii We show that NFAT-1-deficient monocytes unable migrate gondii-infected mice. Moreover, NFAT1-/- mice highly susceptible chronic infection due a failure parasite replication CNS....
In many infectious diseases, the pathogen-induced inflammatory response could result in protective immunity that should be regulated to prevent tissue damage and death. fact, Trypanosoma cruzi infection, innate immune perfectly controlled avoid significant lesions Here, we investigate role of Blimp-1 expression T cells resistance T. infection. Therefore, using mice with deficiency (CKO) determined its controlling parasites growth during acute phase Infection ablation resulted failure...
ABSTRACT Cytolytic CD8 + T cells mediate immunopathology in cutaneous leishmaniasis without controlling parasites. Here, we identify factors involved cell migration to the lesion that could be targeted ameliorate disease severity. CCR5 was most highly expressed chemokine receptor patient lesions, and high expression of CCL3 CCL4, ligands, associated with delayed healing lesions. To test requirement for CCR5, Leishmania- infected Rag1 -/- mice were reconstituted cells. We found these...
Cutaneous leishmaniasis caused by