A5062342343- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Research on Leishmaniasis Studies
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Eosinophilic Esophagitis
- Essential Oils and Antimicrobial Activity
- Health, Nursing, Elderly Care
- Bee Products Chemical Analysis
- Immune cells in cancer
- Vitamin C and Antioxidants Research
- Signaling Pathways in Disease
- Intensive Care Unit Cognitive Disorders
- Immune responses and vaccinations
- Neonatal and Maternal Infections
- S100 Proteins and Annexins
- Parasites and Host Interactions
- Stress Responses and Cortisol
- Pomegranate: compositions and health benefits
- Trypanosoma species research and implications
- Adenosine and Purinergic Signaling
- Urticaria and Related Conditions
Universidade de São Paulo
2016-2023
Universidade de Ribeirão Preto
2020-2022
Centro de Reprodução Humana Prof. Franco Junior
2020
Severe cases of COVID-19 are characterized by a strong inflammatory process that may ultimately lead to organ failure and patient death. The NLRP3 inflammasome is molecular platform promotes inflammation via cleavage activation key molecules including active caspase-1 (Casp1p20), IL-1β, IL-18. Although participation the in has been highly speculated, outcome disease unknown. Here we demonstrate activated response SARS-CoV-2 infection patients. Studying moderate severe patients, found PBMCs...
Although SARS-CoV-2 severe infection is associated with a hyperinflammatory state, lymphopenia an immunological hallmark, and correlates poor prognosis in COVID-19. However, it remains unknown if circulating human lymphocytes monocytes are susceptible to infection. In this study, of peripheral blood mononuclear cells (PBMCs) was investigated both vitro vivo . We found that whole PBMCs from healthy donors productive virus progeny. Results revealed monocytes, as well B T lymphocytes, active...
Abstract Background The release of neutrophil extracellular traps (NETs) is associated with inflammation, coagulopathy, and organ damage found in severe cases COVID-19. However, the molecular mechanisms underlying NETs COVID-19 remain unclear. Objectives We aim to investigate role Gasdermin-D (GSDMD) pathway on development during Methods performed a single-cell transcriptome analysis public data bronchoalveolar lavage. Then, we enrolled 63 hospitalized patients moderate analyze blood lung...
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is associated with a hyperinflammatory state and lymphocytopenia, hallmark that appears as both signature prognosis of disease severity outcome. Although cytokine storm sustained inflammatory are commonly immune cell depletion, it still unclear whether direct SARS-CoV-2 cells could also play role in this scenario by harboring viral replication. We found monocytes, well B T lymphocytes, were susceptible to...
The relationship established between Leishmania infantum and the vertebrate host can lead to a self-healing infection or manifestation of visceral leishmaniasis, chronic systemic associated with high rates mortality. We hypothesized that regulatory cytokines, such as interleukin-27 (IL-27), play role in susceptibility L. infection. IL-27 is heterodimeric cytokine composed IL-27p28 EBi3 subunits which, when combined, bind IL-27R, leading STAT-1 -3 activation, playing regulation immune...
Many studies have reported that patients with psychosis, even before drug treatment, mildly raised levels of blood cytokines relative to healthy controls. In contrast, there is a remarkable scarcity investigating the cellular basis immune function and cytokine changes in psychosis. The few flow-cytometry been limited counting proportion major classes monocyte lymphocytes without distinguishing their pro- anti-inflammatory subsets. Moreover, most investigations are cross-sectional conducted...
Ministério da Saúde, através Política Nacional Atenção Básica (PNAB) recomenda a inclusão do Gerente de com o objetivo contribuir para aprimoramento e qualificação processo trabalho nas UBS. O estudo objetiva analisar adesão dos municípios Região Litoral Leste/Jaguaribe ao por meio CNES. Trata-se um descritivo abordagem quantitativa. Realizado no Cadastro Estabelecimento Saúde que compõem estado Ceará. Das 201 UBS Leste/Jaguaribe, 24,38% das possuem Básica, enquanto 75,62% não são...
Visceral leishmaniasis (VL) is a chronic and fatal disease caused by Leishmania infantum in Brazil. Leukocyte recruitment to infected tissue crucial event for the control of infections such as VL. Among inflammatory cells, neutrophils are recruited site infection, these cells may parasite replication through oxidative or non-oxidative mechanisms. The recruitment, activation functions coordinated pro-inflammatory cytokines chemokines during recognition pattern receptors (PRRs). Here, we...
Abstract Background Although the literature shows that an increase in both number and suppressive function of CD4+forkhead box P3 (FOXP3)+ T-regulatory cells (Tregs) during sepsis contributes to immunosuppressed state, little is known about identity these cells. Methods Using mouse model cecal ligation puncture (CLP), we analyzed frequency molecular signature T-cell immunoglobulin ITIM domain (TIGIT)+ TIGIT− Treg subsets, using flow cytometry quantitative polymerase chain reaction. In...
Adenosine is an endogenously released purine nucleoside that signals through four widely expressed G protein-coupled receptors: A1, A2A, A2B, and A3. Of these, A2AR recognized as mediating major adenosine anti-inflammatory activity. During cutaneous leishmaniasis, induces immunosuppression, which promotes the establishment of infection. Herein, we demonstrated signaling exploited by Leishmania infantum parasites, etiologic agent causes Visceral Leishmaniasis, to successfully colonize...
The TIGIT+FOXP3+Treg subset (TIGIT+Tregs) exerts robust suppressive activity on cellular immunity and predisposes septic individuals to opportunistic infection. We hypothesized that TIGIT+Tregs could play an important role in intensifying the COVID-19 severity hampering defense against nosocomial infections during hospitalization. Herein we aimed verify association between levels of with mechanical ventilation requirement, fatal outcome, bacteremia were immunophenotyped by flow cytometry...
Propolis is known for its immunomodulatory properties. We investigated the effects of three recently developed propolis extract formulations: polar fraction (PPF), soluble dry (PSDE), and microencapsulated (MPE), some their components, on pro- anti-inflammatory cytokine production in a macrophage model. Bone marrow cell-derived macrophages (BMDM) cell culture were E. coli lipopolysaccharide (500 ng/mL) stimulated two hours subsequently incubated 20 with one formulations (1, 10, 25, 50, 100...
Abstract COVID-19 is a disease of dysfunctional immune responses, but the mechanisms triggering immunopathogenesis are not established. The functional plasticity macrophages allows this cell type to promote pathogen elimination and inflammation or suppress tissue remodeling injury repair. During an infection, clearance dead dying cells, process named efferocytosis, can modulate interplay between these contrasting functions. Here, we show that engulfment SARS-CoV2-infected apoptotic cells...
This study investigated the anti-caries and anti-inflammatory effects of mouthwash formulations containing Punica granatum (pomegranate) peel extract (PPE), sodium-trimetaphosphate, low concentrations fluoride. PPE was characterized using high-performance liquid chromatography (ellagic acid punicalagin). Total phenolics were quantified among formulations, their stability analyzed for 28 days. The formulation evaluated as follows: (1) inorganic component concentration reduced demineralization...
Abstract The release of neutrophil extracellular traps (NETs) is associated with inflammation, coagulopathy, and organ damage found in severe cases COVID-19. However, the molecular mechanisms underlying NETs COVID-19 remain unclear. Using a single-cell transcriptome analysis we observed that expression GSDMD inflammasome-related genes were increased neutrophils from patients. Furthermore, high was structures lung tissue activation requires live SARS-CoV-2 occurs after infection via ACE2...
Abstract Background and Purpose Sepsis‐surviving adult individuals commonly develop immunosuppression increased susceptibility to secondary infections, an outcome mediated by the axis IL‐33/ILC2s/M2 macrophages/Tregs. Nonetheless, long‐term immune consequences of paediatric sepsis are indeterminate. We sought investigate role age in genesis following sepsis. Experimental Approach Here, we compared frequency Tregs, activation IL‐33/ILC2s M2 macrophages DNA methylation epithelial lung cells...
Abstract Sepsis survival in adults is commonly followed by immunosuppression and increased susceptibility to secondary infections. However, the long-term immune consequences of pediatric sepsis are unknown. Here, we compared frequency Tregs, activation IL-33/ILC2s axis M2 macrophages, DNA methylation epithelial lung cells from post-septic infant adult mice. In contrast adults, mice were resistant infection did not show impairment tumour controls upon melanoma challenge. Mechanistically,...