A5012451960- Phagocytosis and Immune Regulation
- Immune cells in cancer
- Immune responses and vaccinations
- COVID-19 Clinical Research Studies
- Inflammasome and immune disorders
- Nanoplatforms for cancer theranostics
- IL-33, ST2, and ILC Pathways
- Immune Cell Function and Interaction
- Photodynamic Therapy Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Urticaria and Related Conditions
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Wound Healing and Treatments
- PI3K/AKT/mTOR signaling in cancer
- Complement system in diseases
- Adenosine and Purinergic Signaling
- Cerebrospinal fluid and hydrocephalus
- Inflammatory mediators and NSAID effects
- Antimicrobial Peptides and Activities
- Palliative and Oncologic Care
- Neonatal Respiratory Health Research
- Environmental and biological studies
- Indoor Air Quality and Microbial Exposure
- Ocular Infections and Treatments
- Biological Research and Disease Studies
Vanderbilt University Medical Center
2019-2025
Universidade de São Paulo
2015-2023
Universidade Estadual Paulista (Unesp)
2014-2023
Institute of Molecular and Cell Biology
2023
Vanderbilt University
2022-2023
Universidade de Ribeirão Preto
2019
Centro Universitário de Araraquara
2017
COVID-19 is a disease of dysfunctional immune responses, but the mechanisms triggering immunopathogenesis are not established. The functional plasticity macrophages allows this cell type to promote pathogen elimination and inflammation or suppress tissue remodeling injury repair. During an infection, clearance dead dying cells, process named efferocytosis, can modulate interplay between these contrasting functions. Here, we show that engulfment SARS-CoV-2-infected apoptotic cells exacerbates...
Abstract Infectious pneumonia is a major cause of morbidity/mortality, mainly because the increasing rate microorganisms resistant to antibiotics. Photodynamic Therapy (PDT) emerging as promising approach, effects are based on oxidative stress, preventing microorganism resistance. In two previous studies, in vitro inactivation Streptococcus pneumoniae using indocyanine green (ICG) and infrared light source was success killing 5 log 10 colony‐forming units (CFU/mL) with only μ mol/L ICG. this...
Patients with severe COVID-19 develop acute respiratory distress syndrome (ARDS) that may progress to cytokine storm syndrome, organ dysfunction, and death. Considering complement component 5a (C5a), through its cellular receptor C5aR1, has potent proinflammatory actions plays immunopathological roles in inflammatory diseases, we investigated whether the C5a/C5aR1 pathway could be involved pathophysiology. signaling increased locally lung, especially neutrophils of critically ill patients...
Abstract Phagocytosis requires the coordination of various classes receptors and activation multiple signaling programs, culminating in actin cytoskeletal rearrangement ingestion. Given pleiotropic nature events necessary for proper microbial ingestion, identifying molecules that control distinct steps phagocytosis could reveal potential strategies to enhance clearance. PTEN is a lipid/protein phosphatase traditionally recognized as tumor suppressor. While inhibits arms innate immune...
Significance Citrobacter rodentium infection, a murine colitis model to study human intestinal diseases, causes exacerbated apoptosis of epithelial cells and triggers Th17 immune responses. Here, we identified heretofore unknown role for the bioactive lipid mediator prostaglandin E 2 (PGE ) in inhibition cell differentiation during C. infection. When PGE receptor EP4 was antagonized, detected enhanced colonic cells, increased expression antimicrobial peptides, decreased bacterial numbers...
Significance Production of IL-1β is an essential component the inflammatory response and host defense. secretion dependent on activation intracellular platform termed inflammasome. The initial signals that drive inflammasome remains elusive. Here, we show bioactive lipid leukotriene B 4 enhances both transcriptional posttranscriptional programs activate in vivo vitro. We identified critical signaling required for assembly, secretion, its consequences skin Our data also suggest prevention LTB...
Alveolar macrophages (AMs) are multitasking cells that maintain lung homeostasis by clearing apoptotic (efferocytosis) and performing antimicrobial effector functions. Different PRRs have been described to be involved in the binding capture of non-opsonized Streptococcus pneumoniae, such as TLR-2, mannose receptor (MR) scavenger receptors (SRs). However, mechanism which ingestion negatively influences clearance S. pneumoniae remains determined. In this study, we evaluated whether...
Efferocytosis, or clearance of apoptotic cells (ACs), by dendritic (DCs) leads to immune response suppression and tolerance self-antigens. However, efferocytosis infected (IACs) the production a mixed pro- anti-inflammatory cytokine milieu. We examined DC phenotype ability migrate after phagocytosis ACs IACs observed higher levels CD86 CCR7 expression in DCs, as well enhanced migration capacity following IACs. Interestingly, interleukin-1β, interleukin-10 prostaglandin E2 (PGE2 ) were also...
Abstract Pneumonia is the main cause of children mortality worldwide, and its major treatment obstacle stems from microorganisms increasing development resistance to several antibiotics. Photodynamic therapy has been presenting, for last decades, promising results some subtypes cancer infections. In this work we aimed develop a safe efficient in vitro protocol photodynamic inactivation Streptococcus pneumoniae , one most commonly found bacteria pneumonia cases, using two near‐infrared light...
Abstract COVID-19 is a disease of dysfunctional immune responses, but the mechanisms triggering immunopathogenesis are not established. The functional plasticity macrophages allows this cell type to promote pathogen elimination and inflammation or suppress tissue remodeling injury repair. During an infection, clearance dead dying cells, process named efferocytosis, can modulate interplay between these contrasting functions. Here, we show that engulfment SARS-CoV2-infected apoptotic cells...
Abstract Apoptotic cell clearance by professional and nonprofessional phagocytes in the process of efferocytosis is critical to preserve tissue homeostasis. Uptake apoptotic cells dendritic generates regulatory T induces immunologic tolerance against self-antigens. In contrast, ingestion infected promotes activation TLR4/MyD88-dependent bone marrow–derived (BMDCs) triggers Th17 differentiation. this study, we evaluated impact Streptococcus pneumoniae–infected BMDCs derived from C57BL/6 mice...
The available antifungal therapeutic arsenal is limited. search for alternative drugs with fewer side effects and new targets remains a major challenge. Decyl gallate (G14) derivative of gallic acid range biological activities broad-spectrum activity. Previously, our group demonstrated the promising anti- Paracoccidioides activity G14.
Lower respiratory infections are among the leading causes of death worldwide. In this study, it was evaluated interaction indocyanine green, a photosensitizer activated by infrared light, with alveolar macrophages and effectiveness photodynamic therapy using compound against Streptococcus pneumoniae . Initial experiments analyzed green toxicity to in dark different drug concentrations incubation times, macrophage viability obtained MTT method. The average results showed values below 90% for...
Abstract Patients with severe COVID-19 develop acute respiratory distress syndrome (ARDS) that may progress to cytokine storm syndrome, organ dysfunction, and death. Considering complement component 5a (C5a), through its cellular receptor C5aR1, has potent proinflammatory actions, plays immunopathological roles in inflammatory diseases, we investigated whether C5a/C5aR1 pathway could be involved pathophysiology. signaling increased locally the lung, especially neutrophils of critically ill...
Abstract BACKGROUND The events that initiate inflammatory responses during infections are not well understood. lipid mediator leukotriene B4 (LTB4) is produced within seconds to minutes after phagocyte challenge and emerging evidence shown LTB4 increases antimicrobial effector functions mediators, such as IL1b. Inflammasomes intracellular platforms required for the secretion of IL1B/IL18 an cell death termed pyroptosis. Whether enhances inflammasome-dependent IL1b remains be fully Here, we...
Abstract Sepsis is a life-threatening condition characterized by systemic inflammation, multiple organ failure, and death. Macrophage metabolism actions play critical role in sepsis pathogenesis. Fatty acid was identified as one of the most promising metabolic predictors for survival sepsis. The phosphatase tensin homolog (PTEN) regulator cell growth influences different arms innate immune response. Here, we seek to investigate whether PTEN bridge between immunometabolism gene expression...
Abstract Staphylococcus aureus (MRSA) skin infections are characterized by the initial recruitment of neutrophils followed establishment a localized abscess. An abscess comprises fibrous capsule, viable and apoptotic neutrophils, bacteria in core. Along with eliminating bacteria, clearance dead (efferocytosis) is crucial to restoring tissue homeostasis. Efferocytosis tightly regulated process initiated when dying cells secrete “find-me” signals such as Sphingosine 1 phosphate (S1P) that...
Abstract Sepsis is defined as life-threatening organ dysfunction and damage caused by an aberrant host immune response to systemic infection. Due the abundant production of different classes inflammatory mediators, investigating role endogenous proteins that influence multiple signaling pathways might provide important clues in understanding sepsis pathogenesis. Phosphatase tensin homolog (PTEN) a protein/lipid phosphatase, tumor suppressor, negative regulator PI3K/AKT/mTORC pathway. PTEN...
Clearance of dying cells, named efferocytosis, is a pivotal function professional phagocytes that impedes the accumulation cell debris. Efferocytosis can be experimentally assessed by differentially tagging target cells and analyzing imaging or flow cytometry. Here, we describe an assay to evaluate uptake apoptotic (ACs) human macrophages in vitro labeling different with commercially available dyes analysis We detail methods prepare label lymphocytes approach determine AC uptake. This...