A5028475368- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Plant-Microbe Interactions and Immunity
- Studies on Chitinases and Chitosanases
- Tannin, Tannase and Anticancer Activities
- Antimicrobial Peptides and Activities
- Plant Pathogens and Fungal Diseases
- Nail Diseases and Treatments
- Invertebrate Immune Response Mechanisms
- Nanoplatforms for cancer theranostics
- Phytoplasmas and Hemiptera pathogens
- Bioactive Compounds and Antitumor Agents
- Advanced biosensing and bioanalysis techniques
- Nanoparticles: synthesis and applications
- Monoclonal and Polyclonal Antibodies Research
- Natural Compounds in Disease Treatment
- Essential Oils and Antimicrobial Activity
- Bacterial biofilms and quorum sensing
- Mycotoxins in Agriculture and Food
- Advanced Nanomaterials in Catalysis
- Phytochemical compounds biological activities
- Oral microbiology and periodontitis research
- Photodynamic Therapy Research Studies
Universidade Estadual Paulista (Unesp)
2012-2022
Centro Universitário de Araraquara
2012-2015
Fungal infections in humans have increased alarmingly recent years, particularly immunocompromised individuals. Among the systemic candidiasis, aspergillosis, cryptococcosis, paracoccidioidomycosis, and histoplasmosis mortality are more prevalent severe humans. The current high incidence of dermatophytosis is humans, especially as main etiologic agents Trichophyton rubrum mentagrophytes . Molecules pristimerin maytenin obtained from plant Maytenus ilicifolia (Celastraceae) known to show...
Members of the Paracoccidioides genus are dimorphic fungi that etiologic agents paracoccidioidomycosis (PCM). This is composed two species: brasiliensis and lutzii. The correct molecular taxonomic classification these has created new opportunities for studying understanding their relationships with hosts. spp. have features permit growth under adverse conditions, enable them to adhere invade host tissues may contribute disease development. Cell wall proteins called adhesins facilitate...
Paracoccidioidomycosis is a systemic mycosis, endemic in Latin America. The etiologic agents of this mycosis are composed 2 species: Paracoccidioides brasiliensis and P. lutzii. Murine animal models the gold standard for vivo studies; however, ethical, economical logistical considerations limit their use. Galleria mellonella suitable model studies fungal infections. In study, we compared virulence lutzii G. model. deaths larvae infected with or were similar, both species able to reduce...
ABSTRACT CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution and added alamarBlue reagent to test responses brasiliensis lutzii against amphotericin B itraconazole antifungals. The proved be sensitive, practical, inexpensive can used monitor activity low-growth microorganisms their response various drugs.
Paracoccidioides brasiliensis and lutzii are dimorphic fungi the etiological agents of paracoccidioidomycosis (PCM). Adhesion is one most important steps in infections with responsible for differences virulence isolates these fungi. Because importance adhesion to establishment an infection, this study focused on preliminary development a new therapeutic strategy inhibit by Paracoccidioides, thus inhibiting infection preventing disease. We used two phage display libraries select peptides that...
In the current COVID-19 pandemic, next generation of innovative materials with enhanced anti-SARS-CoV-2 activity is urgently needed to prevent spread this virus within community. Herein, we report synthesis chitosan/α-Ag2WO4 composites synthetized by femtosecond laser irradiation. The antimicrobial against Escherichia coli, Methicilin-susceptible Staphylococcus aureus (MSSA), and Candida albicans was determined estimating minimum inhibitory concentration (MIC) minimal bactericidal/fungicidal...
This study was aimed at analyzing proto-oncogenic signaling pathway activation in normal oral keratinocytes (NOK-si) and neoplastic cell lines (SCC 25 Detroit 562) stimulated with metabolites (soluble factors) from single dual biofilms of Candida albicans Staphylococcus aureus . Soluble factors (SF) early (16-h) mature (36-h) C. S. were collected incubated cultures, which subsequently evaluated using gene expression via RT-qPCR, viability AlamarBlue TM , flow cytometry cycle analysis. In...
This work aims to demonstrate that the gallic acid structure modification decyl gallate (G14) compound contributed increase antifungal activity against several species of pathogenic fungi, mainly, Candida spp., Cryptococcus Paracoccidioides and Histoplasma capsulatum, according standardized microdilution method described by Clinical Laboratory Standard Institute (CLSI) documents. Moreover this has a particularly good selectivity index value, which makes it an excellent candidate for...
Abstract The genus Paracoccidioides consist of dimorphic fungi geographically limited to the subtropical regions Latin America, which are responsible for causing deep systemic mycosis in humans. However, molecular mechanisms by spp. causes disease remain poorly understood. harbor genes that encode proteins involved host cell interaction and mitochondrial function, together required pathogenicity mediate virulence. Previously, we identified TufM (previously known as EF-Tu) brasiliensis...
Paracoccidioidomycosis (PCM) is a fungal disease restricted to Latin countries, and its etiologic agents derive from the Paracoccidioides genus. Attenuation or loss of virulence in spp. following successive subculturing has been described. However, can be recovered by passage mammalian host. In this study, recovery adhesion P. brasiliensis through mice was compared that insect Galleria mellonella. Analysis vitro fungal-host cell interaction, gene expression adhesins, analysis survival curves...
Background: Paracoccidioidomycosis (PCM) is a chronic disease that causes sequelae and requires prolonged treatment; therefore, new therapeutic approaches are necessary. In view of this, three peptides from Paracoccidioides brasiliensis 14-3-3 protein were selected based on its immunogenicity potential. Methods: The in vitro antifungal activity cytotoxicity the evaluated. influence immunological survival aspects was evaluated vivo, using Galleria mellonella expression antimicrobial peptide...
The available antifungal therapeutic arsenal is limited. search for alternative drugs with fewer side effects and new targets remains a major challenge. Decyl gallate (G14) derivative of gallic acid range biological activities broad-spectrum activity. Previously, our group demonstrated the promising anti- Paracoccidioides activity G14.
Apoptosis is considered an escape mechanism from the host immune system for fungus Paracoccidioides spp, and it serves as a vehicle entry into macrophages without stimulating microbicidal activities. Recently, gp43 of P. brasiliensis was demonstrated to be involved in this process. Therefore, new therapeutic alternative, very important study compounds that could reduce modulation induction apoptosis caused by fungus. Decyl gallate (G14) known antifungal compound, we decided investigate its...