A5040008549- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Mosquito-borne diseases and control
- COVID-19 Clinical Research Studies
- Malaria Research and Control
- Computational Drug Discovery Methods
- Synthesis and biological activity
- HIV Research and Treatment
- Viral Infections and Vectors
- Virology and Viral Diseases
- Essential Oils and Antimicrobial Activity
- Herpesvirus Infections and Treatments
- Long-Term Effects of COVID-19
- interferon and immune responses
- SARS-CoV-2 detection and testing
- Viral Infections and Immunology Research
- Inflammasome and immune disorders
- Genomics and Phylogenetic Studies
- Organoselenium and organotellurium chemistry
- Vibrio bacteria research studies
- Viral Infections and Outbreaks Research
- Education during COVID-19 pandemic
- Animal Disease Management and Epidemiology
- RNA and protein synthesis mechanisms
Fundação Oswaldo Cruz
2016-2025
Universidade Federal do Rio de Janeiro
2014-2022
International Olive Council
2019
Universidade Federal Fluminense
2007-2012
Hospital del Niño
2009
Viruses are obligate intracellular parasites that make use of the host metabolic machineries to meet their biosynthetic needs. Thus, identifying pathways essential for virus replication may lead potential targets therapeutic intervention. The mechanisms and explored by SARS-CoV-2 support its within cells not fully known. Lipid droplets (LD) organelles with major functions in lipid metabolism, energy homeostasis transport, have multiple roles infections inflammation. Here we described...
Infection by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been associated with leukopenia and uncontrolled inflammatory response in critically ill patients. A better comprehension of SARS-CoV-2-induced monocyte death is essential for identification therapies capable to control hyper-inflammation reduce viral replication patients 2019 disease (COVID-19). Here, we show that SARS-CoV-2 engages inflammasome triggers pyroptosis human monocytes, experimentally infected,...
Zika virus (ZIKV) is a member of the Flaviviridae family, along with other agents clinical significance such as dengue (DENV) and hepatitis C (HCV) viruses. Since ZIKV causes neurological disorders during fetal development in adulthood, antiviral drugs are necessary. Sofosbuvir clinically approved for use against HCV targets protein that most conserved among members viral RNA polymerase. Indeed, we found sofosbuvir inhibits polymerase, targeting amino acid residues. inhibited replication...
An unprecedented global outbreak caused by the monkeypox virus (MPXV) prompted World Health Organization to declare a public health emergency of international concern on July 23, 2022. Therapeutics and vaccines for MPXV are not widely available, necessitating further studies, particularly in drug repurposing area. To this end, standardization vitro infection systems is essential. The most robust studies poxviruses Vaccinia virus, there significant gaps understanding replicative cycle MPXV....
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is already responsible for far more deaths than previous pathogenic coronaviruses (CoVs) from 2002 and 2012. The identification of clinically approved drugs to be repurposed combat 2019 CoV disease (COVID-19) would allow the rapid implementation potentially life-saving procedures. major protease (Mpro) SARS-CoV-2 considered a promising target, based on results related CoVs with lopinavir (LPV), an HIV inhibitor.
Summary Genomic surveillance has become a useful tool for better understanding virus pathogenicity, origin and spread. Obtaining accurately assembled, complete viral genomes directly from clinical samples is still challenging. Here, we describe three protocols using unique primer set designed to recover long reads of SARS-CoV-2 total RNA extracted samples. This protocol useful, accessible adaptable laboratories with varying resources access distinct sequencing methods: Nanopore, Illumina...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is already responsible for far more deaths than previous pathogenic coronaviruses (CoVs) from 2002 and 2012. The identification of clinically approved drugs to be repurposed combat 2019 CoV disease (COVID-19) would allow the rapid implementation potentially life-saving procedures. major protease (Mpro) SARS-CoV-2 considered a promising target, based on results related CoVs with lopinavir (LPV), an HIV inhibitor. However,...
The SARS-CoV-2 pandemic has prompted global efforts to develop therapeutics. main protease of (Mpro) and the papain-like (PLpro) are essential for viral replication key targets therapeutic development. In this work, we investigate mechanisms inhibition by diphenyl diselenide (PhSe)2 which is an archetypal model diselenides a renowned potential agent. in vitro inhibitory concentration against Vero E6 cells falls low micromolar range. Molecular dynamics (MD) simulations density functional...
Abstract Brazil, which is hyperendemic for dengue virus (DENV), has had recent Zika (ZIKV) and (CHIKV) Chikungunya outbreaks. Since March 2016, CHIKV the arbovirus infection most frequently diagnosed in Rio de Janeiro. In analysis of 1835 syndromic patients, screened by real time RT-PCR, 56.4% cases were attributed to CHIKV, 29.6% ZIKV, 14.1% DENV-4. Sequence analyses from sixteen samples revealed that East-Central-South-African ( ECSA) genotype been circulating Brazil since 2013 [95%...
COVID-19 disease, triggered by SARS-CoV-2 virus infection, has led to more than 7.0 million deaths worldwide, with a significant fraction of recovered infected people reporting postviral symptoms. Smart surfaces functionalized nanoparticles are powerful tool inactivate the and prevent further spreading disease. Literature reports usually focus on role nanomaterial composition size dispersion in evaluating their efficacy against SARS-CoV-2. Here, anti-SARS-CoV-2 activity oleylamine (OAm) used...
The COVID-19 pandemic has caused over 7 million deaths globally in the past four years. Siparuna spp. (Siparunaceae), which is used Brazilian folk medicine, considered a genus with potential antiviral alternatives. This study explored correlation between phytochemicals leaf extracts (S. ficoides, S. decipiens, glycycarpa, reginae, and cymosa) their against various SARS-CoV-2 targets. In vitro assays examined interactions spike protein ACE2 receptor, protease activity, viral replication...
The SARS-CoV-2 proteases Mpro and PLpro are critical targets for antiviral drug development the treatment of COVID-19. 1,2,4-thiadiazole functional group is an inhibitor cysteine proteases, such as papain cathepsins. This chemical moiety also present in ceftaroline fosamil (CF), FDA-approved fifth-generation cephalosporin antibiotic. study investigates interactions between CF, its primary metabolites (M1 dephosphorylated CF M2 opened β-lactam ring) derivatives (protonated M1H M2H), open...
Despite extensive efforts, no highly effective antiviral molecule exists for treating moderate and severe COVID-19. Nanotechnology has emerged as a promising approach developing novel drug delivery systems to enhance efficacy. Among these, polymeric nanomicelles improve the solubility, bioavailability, cellular uptake of therapeutic agents. In this study, Pluronic F127-based were developed evaluated their activity against SARS-CoV-2. The nanomicelles, formulated using direct dissolution...
The influenza virus can cause seasonal infections with mild to severe symptoms, circulating worldwide, and it affect people in any age group. Therefore, this infection is a serious public health problem that causes illness death high-risk populations. Every year, 0.5% of the world's population infected by pathogen. This percentage increase up ten times during pandemics. Influenza vaccination most effective way prevent disease. In addition, anti-influenza drugs are essential for prophylactic...
The coronaviruses (CoVs) called the attention of world for causing outbreaks severe acute respiratory syndrome (SARS-CoV), in Asia 2002-03, and disease Middle East (MERS-CoV), 2012. In December 2019, yet again a new coronavirus (SARS-CoV-2) first identified Wuhan, China, was associated with infection, known today as COVID-19. This virus quickly spread throughout China 30 additional countries. As result, World Health Organization (WHO) elevated status COVID-19 outbreak from emergency...
The pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has impacted public health and world economy fueled a worldwide race to approve therapeutic prophylactic agents, but so far there are no specific antiviral drugs. Understanding biology of virus is first step in structuring strategies combat it, this context several studies have been conducted with aim understanding replication mechanism SARS-CoV-2 vitro systems. In work, using transmission scanning...
Aurones are a small subgroup of flavonoids in which the basic C6–C3–C6 skeleton is arranged as (Z)-2-benzylidenebenzofuran-3(2H)-one. These compounds structural isomers flavones and flavonols, natural products reported potent inhibitors SARS-CoV-2 replication. Herein, we report design, synthesis, anti-SARS-CoV-2 activity series 25 aurones bearing different oxygenated groups (OH, OCH3, OCH2OCH3, OCH2O, OCF2H, OCH2C6H4R) at A- and/or B-rings using cell-based screening assays. We observed that...
Despite the development of specific therapies against severe acute respiratory coronavirus 2 (SARS-CoV-2), continuous investigation mechanism action clinically approved drugs could provide new information on druggable steps virus–host interaction. For example, chloroquine (CQ)/hydroxychloroquine (HCQ) lacks in vitro activity SARS-CoV-2 TMPRSS2-expressing cells, such as human pneumocyte cell line Calu-3, and likewise, failed to show clinical benefit Solidarity Recovery trials. Another...
SARS-CoV-2 is the causative agent of COVID-19 and responsible for current global pandemic. The viral genome contains 5 major open reading frames which largest ORF1ab codes two polyproteins, pp1ab pp1a, are subsequently cleaved into 16 nonstructural proteins (nsp) by cysteine proteases encoded within polyproteins. main protease (Mpro, nsp5) cleaves majority nsp's, making it essential replication has been successfully targeted development antivirals. first oral Mpro inhibitor, nirmatrelvir,...
Traditional medicine shows several treatment protocols for COVID-19 based on natural products, revealing its potential as a possible source of anti-SARS-CoV-2 agents. Ampelozizyphus amazonicus is popularly used in the Brazilian Amazon fortifier and tonic, recently, it has been reported to relieve symptoms. This work aimed investigate antiviral A. amazonicus, focusing inhibition spike ACE2 receptor interaction, key step successful infection. Although saponins are major compounds this plant...