A5103029360- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Virology and Viral Diseases
- Malaria Research and Control
- SARS-CoV-2 and COVID-19 Research
- Vector-Borne Animal Diseases
- Viral Infections and Outbreaks Research
- Parvovirus B19 Infection Studies
- COVID-19 Clinical Research Studies
- Virus-based gene therapy research
- Electron and X-Ray Spectroscopy Techniques
- COVID-19 epidemiological studies
- Viral Infections and Immunology Research
Fundação Oswaldo Cruz
2015-2024
Mammarenavirus species are associated with a specific rodent host species, although an increasing number of virus has been to more than one host, suggesting that co-evolution is less robust initially thought. There few eco-epidemiological studies South America mammarenaviruses in non-endemic areas Arenavirus Hemorrhagic Fever, affecting specially our current knowledge about animal reservoirs and range host-virus relations. In Brazil, seven arenavirus were described different species. Here we...
The coronaviruses (CoVs) called the attention of world for causing outbreaks severe acute respiratory syndrome (SARS-CoV), in Asia 2002-03, and disease Middle East (MERS-CoV), 2012. In December 2019, yet again a new coronavirus (SARS-CoV-2) first identified Wuhan, China, was associated with infection, known today as COVID-19. This virus quickly spread throughout China 30 additional countries. As result, World Health Organization (WHO) elevated status COVID-19 outbreak from emergency...
Zika virus (ZIKV) is a member of the flavivirus genus, and its genome approximately 10.8 kilobases positive-strand RNA enclosed in capsid surrounded by membrane. Studies on replication dynamics ZIKV are scarce, which limits development antiviral agents vaccines directed against ZIKV. In this study, Aedes albopictus mosquito lineage cells (C6/36 cells) African green monkey kidney epithelial (Vero were inoculated with sample isolated from Brazilian patient, infection was characterized...
In cell culture, structures suffer strong impact due to centrifugation during processing for electron microscope observation. order minimise this effect, a new protocol was successfully developed. Using conventional reagents and equipments, it took over one week, but compression reduced none or the lowest deformation possible.
Zika virus (ZIKV) has infected thousands of Brazilian people and spread to other American countries since 2015. The introduction ZIKV brought a strong impact public health in Brazil. It is utmost importance identify susceptible cell line that will enable the isolation identification from patient samples, viral mass production, testing drug vaccine candidates. Besides real-time reverse transcriptase polymerase chain reaction diagnosis for detecting genome, lines was useful order study...
BACKGROUND The impact of arbovirus cocirculation in Brazil is unknown. Dengue virus (DENV) reinfection may result more intense viraemia or immunopathology, leading to severe disease. Zika (ZIKV) epidemic the Americas provided pathogenicity evidence that had not been previously observed flavivirus infections. In contrast other flaviviruses, electron microscopy studies have shown ZIKV replicate viroplasm-like structures. Flaviviruses produce an ensemble structurally different virions,...
In 2015-2016, the Zika virus (ZIKV) caused a major epidemic in Americas, increasing cases of microcephaly and Guillain-Barré syndrome. During this period, discovery ZIKV sexual transmission intensified studies on impact reproductive organs. For study, 2-month-old male BALB/c mice were infected with 1.26 x 106 PFU/mL solution via intravenous route. After three, seven, fourteen days post-infection (DPI), blood testicle samples obtained to detect RNA. The authors observed that animals had...
Objetivo: Investigar na literatura modelos animais utilizados para pesquisa com o vírus Zika em que haja a possibilidade de quantificação carga viral e análises histopatológicas. Métodos: Trata-se uma revisão sistemática redigida acordo as diretrizes do método PRISMA (2020). Os autores realizaram busca inicial artigos nas plataformas PubMed, Embase, Google Acadêmico, LILACS Scielo. Resultados: Foram encontrados inicialmente 735 artigos, dos quais 27 foram incluídos após avaliação pelos...
Zika Virus propagation
Abstract The aim of the present study was to infect BALB/c mice with ZIKV evaluate clinical signs and quantify viral load in different tissues at three kinetic points. For this purpose, fifteen were inoculated a 100µl solution five other used as negative controls. After euthanasia, nine collected macerated for RNA extraction quantification. infections not lethal. Some them, however, showed great agitation, hair bristling itchy skin. Viral detected one heart sample, eight samples spleen two...
Our group infected BALB/cAn mice with Zika virus to evaluate clinical signs and viral load in several tissues at three different kinetic points.We inoculated fifteen a 100µl of solution collect nine tissues, from each animal, for RNA extraction quantification.Infections caused no lethality.Some them, however, showed great agitation, hair bristling, itchy skin.Viral was detected one sample heart, eight the spleen, two skeletal muscle.Seven positive detections were third day after...