A5070797450- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Nail Diseases and Treatments
- Antimicrobial Peptides and Activities
- Plant Pathogens and Fungal Diseases
- Phytochemistry Medicinal Plant Applications
- Essential Oils and Antimicrobial Activity
- Invertebrate Immune Response Mechanisms
- Probiotics and Fermented Foods
- Plant-Microbe Interactions and Immunity
- Studies on Chitinases and Chitosanases
- Aquaculture disease management and microbiota
- Tannin, Tannase and Anticancer Activities
- Coconut Research and Applications
- Bacterial biofilms and quorum sensing
- Phytochemical compounds biological activities
- Oral microbiology and periodontitis research
- Gut microbiota and health
- Antimicrobial Resistance in Staphylococcus
- Insect symbiosis and bacterial influences
- Healthcare Regulation
- Fungal Biology and Applications
- Traditional and Medicinal Uses of Annonaceae
- Healthcare during COVID-19 Pandemic
- Entomopathogenic Microorganisms in Pest Control
Guarulhos University
2022-2024
Universidade Estadual Paulista (Unesp)
2015-2024
Centro Universitário de Araraquara
2011-2015
Centro Nacional de Microbiologia
2011-2014
Instituto de Salud Carlos III
2011-2014
Instituto de Ciências Farmacêuticas
2014
"Amphotericin B acts through pore formation at the cell membrane after binding to ergosterol" is an accepted dogma about action mechanism of this antifungal, and sentence widely found in literature. But 60 years investigation, Amphotericin not fully elucidated. a polyene substance that one most effective drugs for treatment fungal parasite infections. As stated above, first described was ergosterol present membrane. it has also been demonstrated AmB induces oxidative damage cells. Moreover,...
The incidence of opportunistic fungal infections has increased in recent decades due to the growing proportion immunocompromised patients our society. Candida krusei been described as a causative agent disseminated susceptible patients. Although its prevalence remains low among yeast (2–5%), intrinsic resistance fluconazole makes this important from epidemiologic aspects. Non mammalian organisms are feasible models study virulence and drug efficacy. In work we have used lepidopteran Galleria...
Galleria mellonella is a well-accepted insect model for the study of pathogen-host interactions and antimicrobial compounds. The main advantages this include low cost maintenance, fast life cycle, possibility using large number caterpillars innate immune system, which evolutionarily conserved relative to mammals. Because these advantages, different research groups have been working implement rearing G. in laboratory conditions. This protocol describes our experience experimental infection...
Methodology for testing natural compounds determination of antifungal activity had been developed with adaptations. The most used are bioautography and agar diffusion a complex no defined media. In this study, different methods products discussed the use M27-A2 microdilution test from CLSI (Clinical Laboratory Standards Institute, 2002), as general standard methodology plant extracts is recommended.
Cryptococcus neoformans and gattii are fungal pathogens that most commonly found in infections of the central nervous system, which cause life-threatening meningoencephalitis can grow as a biofilm. Biofilms structures conferring protection resistance microorganism to antifungal drugs. This study compared virulence planktonic biofilm cells C. Galleria mellonella model, well as, quantification gene transcripts LAC1, URE1 CAP59 by real time PCR. All three genes showed significantly increased...
Cryptococcus neoformans is an encapsulated opportunistic fungal pathogen that found in multiple niches the environment and can cause fatal meningoencephalitis susceptible patients, mainly HIV+ individuals. also infects environmental hosts such as nematodes, insects plants. In particular, C. kill lepidopteran Galleria mellonella, which offers a useful tool to study microbial virulence drug efficacy. mellonella immunity relies on innate responses based melanization, accumulation of...
Members of the Paracoccidioides genus are dimorphic fungi that etiologic agents paracoccidioidomycosis (PCM). This is composed two species: brasiliensis and lutzii. The correct molecular taxonomic classification these has created new opportunities for studying understanding their relationships with hosts. spp. have features permit growth under adverse conditions, enable them to adhere invade host tissues may contribute disease development. Cell wall proteins called adhesins facilitate...
Paracoccidioidomycosis is a systemic mycosis, endemic in Latin America. The etiologic agents of this mycosis are composed 2 species: Paracoccidioides brasiliensis and P. lutzii. Murine animal models the gold standard for vivo studies; however, ethical, economical logistical considerations limit their use. Galleria mellonella suitable model studies fungal infections. In study, we compared virulence lutzii G. model. deaths larvae infected with or were similar, both species able to reduce...
Abstract Background and objective In the last decade, numerous studies have been published to clarify role of probiotics, especially Lactobacillus reuteri , as an adjunct conventional periodontal treatment. Although health benefits probiotics are numerous, they live bacteria, administration organisms is not risk‐free. We evaluated antimicrobial effect L its cell‐free culture supernatant on Porphyromonas gingivalis, a keystone pathogen, in vitro. also influence this probiotic live,...
ABSTRACT CLSI method M27-A3 is not available for use with dimorphic fungi, such as those of the Paracoccidioides genus. In this study, we developed a microdilution and added alamarBlue reagent to test responses brasiliensis lutzii against amphotericin B itraconazole antifungals. The proved be sensitive, practical, inexpensive can used monitor activity low-growth microorganisms their response various drugs.
Paracoccidioides brasiliensis and lutzii are dimorphic fungi the etiological agents of paracoccidioidomycosis (PCM). Adhesion is one most important steps in infections with responsible for differences virulence isolates these fungi. Because importance adhesion to establishment an infection, this study focused on preliminary development a new therapeutic strategy inhibit by Paracoccidioides, thus inhibiting infection preventing disease. We used two phage display libraries select peptides that...
Aim: Gallic acid and its ester derivatives have shown antifungal activity in vitro. This study was performed to investigate their against Candida albicans toxicity the animal models Caenorhabditis elegans zebrafish embryos. Results: The compounds protected worms from C. infection. dodecyl gallate most effective. In embryo, gallic were least toxic. Conclusion: potential for vivo use effects of esters these alternative dependent on carbon chain length.