- Insect Pest Control Strategies
- Entomopathogenic Microorganisms in Pest Control
- Vector-borne infectious diseases
- Insect and Pesticide Research
- Insect Resistance and Genetics
- Vector-Borne Animal Diseases
- Helminth infection and control
- Mosquito-borne diseases and control
- Genetic and phenotypic traits in livestock
- Viral Infections and Vectors
- Parasite Biology and Host Interactions
- Fungal Infections and Studies
- Genetic Mapping and Diversity in Plants and Animals
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
- Toxin Mechanisms and Immunotoxins
- Antifungal resistance and susceptibility
- Antimicrobial Peptides and Activities
- Parasitic Infections and Diagnostics
- Rabbits: Nutrition, Reproduction, Health
- Animal Genetics and Reproduction
- Wheat and Barley Genetics and Pathology
- Nematode management and characterization studies
- Livestock Management and Performance Improvement
Brazilian Agricultural Research Corporation
2015-2025
Centro de Ensino Superior de Juiz de Fora
2023
Universidade Federal de Juiz de Fora
2007-2013
Empresa de Pesquisa Agropecuária do Rio Grande do Norte
2010
St. Eugenio Hospital
2008
Universidade Federal Rural do Rio de Janeiro
1999
Galleria mellonella is a well-accepted insect model for the study of pathogen-host interactions and antimicrobial compounds. The main advantages this include low cost maintenance, fast life cycle, possibility using large number caterpillars innate immune system, which evolutionarily conserved relative to mammals. Because these advantages, different research groups have been working implement rearing G. in laboratory conditions. This protocol describes our experience experimental infection...
Streptococcus mutans and Candida albicans are found together in the oral biofilms on dental surfaces, but little is known about ecological interactions between these species. Here, we studied effects of S. UA159 growth pathogencity C. albicans. Initially, biofilm formation morphogenesis were tested vitro. Next, investigate influence pathogenicity using vivo host models, which experimental candidiasis was induced G. mellonella larvae analyzed by survival curves, count hemolymph,...
In this study, we evaluated the interactions between Candida albicans, krusei and glabrata in mixed infections. Initially, these were studied biofilms formed vitro. CFU/mL values of C. albicans lower when compared to single biofilms, verifying 77% 89% reduction species was associated with krusei, respectively. After that, expanded study for vivo host models experimental candidiasis. G. mellonella larvae inoculated monotypic heterotypic suspensions analysis survival rate quantification fungal...
Cryptococcus neoformans and gattii are fungal pathogens that most commonly found in infections of the central nervous system, which cause life-threatening meningoencephalitis can grow as a biofilm. Biofilms structures conferring protection resistance microorganism to antifungal drugs. This study compared virulence planktonic biofilm cells C. Galleria mellonella model, well as, quantification gene transcripts LAC1, URE1 CAP59 by real time PCR. All three genes showed significantly increased...
In tropical countries, losses caused by bovine tick Rhipicephalus (Boophilus) microplus infestation have a tremendous economic impact on cattle production systems. Genetic variation between Bos taurus and indicus to resistance molecular biology tools might allow for the identification of markers linked traits that could be used as an auxiliary tool in selection programs. The objective this work was identify QTL associated with resistance/susceptibility F2 population derived from Gyr (Bos...
The present study evaluated, for the first time, effect of commercial formulation Metarril(®) SP Organic Metarhizium anisopliae plus 10% mineral oil to control Rhipicephalus microplus in a pen study. Three groups were formed with six animals each: group was exposed and 1% Tween 80; second sterile distilled water, 80 (oil group); third received no treatment (control group). fungal contained 1 × 10(8)conidiaml(-1). Each animal sprayed 3L formulation. Fallen ticks counted daily sample 20...
The monitoring of resistance cattle tick populations in Brazil to the chemical bases use is largely limited investigation phenotypic profile. There are few studies investigating role played by genotypic profile acaricide country. Therefore, aim present study was carry out molecular characterization and trace genetic Rhipicephalus microplus with respect organophosphate pyrethroid groups. For that purpose, larvae were genotyped belonging 587 for pyrethroids 306 organophosphates, using...
The acaricidal activity of combinations thymol, carvacrol and eugenol was evaluated on larvae engorged females the cattle tick Rhipicephalus microplus. first step assessed compounds separately, in concentrations 3.125, 6.25, 12.5 25 mg/mL. Then tests were performed with combined ratio 1:1 at 3.125 6.25 mg/mL, along control group treated solvent (3% DMSO). In second step, tested incorporated a formulation concentration de using larval packet adult immersion tests. associations + thymol (3.125...
The aim of this study was to evaluate the effect different concentrations entomopathogenic nematode (EPN) Heterorhabditis indica, LPP1, on reproductive biology engorged females Rhipicephalus microplus. For purpose, 240 were divided into eight groups with statistically similar weights (p > 0.05). Each group six subgroups five distributed in Petri dishes and exposed 0, 375, 750, 1500, 3000, 6000, 12,000 24,000 EPNs/dish. following biological parameters evaluated: egg mass weight (mg),...
This study evaluates milk producers' knowledge regarding cattle ticks and practices for controlling them. Ninety-three dairymen in Minas Gerais were interviewed. These producers had no information acaricide efficiency tests. To analyze the information, open responses categorized through "content analysis", descriptive analysis consisting of extracting profile highlighted by highest frequencies. The association between schooling level was tested means chi-square trend It observed that 92.3%...
The aim of this study was to assess the influence different exposure times engorged female Rhipicephalus microplus infective juveniles Heterorhabditis bacteriophora isolate HP88. females were divided into seven groups (six treatments and one control) 20 ticks each with statistically similar average weights (p>0.05) exposed concentrations 300 nematodes/tick for periods 3, 6, 12, 24, 48 72 h. following biological parameters monitored: pre-oviposition period; egg mass weight; hatching...