Destruxins (DTXs) are cyclic depsipeptides produced by many Metarhizium isolates that have long been assumed to contribute virulence of these entomopathogenic fungi. We evaluated the 20 against insect larvae and measured concentration DTXs A, B, E same in submerged (shaken) cultures. Eight (ARSEF 324, 724, 760, 1448, 1882, 1883, 3479, 3918) did not produce or during five days culture. were first detected culture medium at 2–3 Galleria mellonella Tenebrio molitor showed considerable variation...

This study aimed to evaluate the in vitro effect of entomopathogenic fungi Metarhizium anisopliae sensu lato (s.l.) and Beauveria bassiana on two distinct populations Rhipicephalus microplus, from different experimental farms. Bioassays were performed with engorged females, eggs larvae. Fungal infection was evaluated based biological parameters treated percentage hatch eggs, mortality mean lethal time (LT90) When treatments compared between populations, there significant differences...

The formulations of acaripathogenic fungi to control ticks have been widely studied. present study evaluated the efficacy oil-based Metarhizium anisopliae sensu lato (s.l.), isolate Ma 959, and Beauveria bassiana, Bb 986, on different Rhipicephalus microplus stages, comparing between aqueous suspensions 10, 15 20% mineral oil formulations. Twelve groups were formed: one group; three groups, at or 20%; two fungal M. s.l. B. bassiana; (s.l.) bassiana containing 15, oil. To prepare oily...

The tick Rhipicephalus microplus poses a serious threat to the cattle industry, resulting in economic losses aggravated by resistance chemical acaricides. Strains of Metarhizium spp., well-known group entomopathogenic fungi, can contribute managing this ectoparasite. We explored two novel granular, microsclerotia- or blastospores-based formulations robertsii for R. control under semi-field conditions. Fungal persistence soil was also observed 336 days. experiment used pots Urochloa decumbens...

The present study evaluated, for the first time, effect of commercial formulation Metarril(®) SP Organic Metarhizium anisopliae plus 10% mineral oil to control Rhipicephalus microplus in a pen study. Three groups were formed with six animals each: group was exposed and 1% Tween 80; second sterile distilled water, 80 (oil group); third received no treatment (control group). fungal contained 1 × 10(8)conidiaml(-1). Each animal sprayed 3L formulation. Fallen ticks counted daily sample 20...

Mosquito-borne diseases affect millions of people. Chemical insecticides are currently employed against mosquitoes. However, many cases insecticide resistance have been reported. Entomopathogenic fungi (EPF) demonstrated potential as a bioinsecticide. Here, we assessed the invasion EPF Beauveria bassiana into Aedes aegypti larvae and changes in activity phenoloxidase (PO) proxy for general activation insect innate immune system. In addition, other cellular humoral responses were...

We assessed the effect of entomopathogenic fungus Metarhizium anisopliae against Aedes aegypti. Conidia M. strains CG 489, 153, and IBCB 481 were grown in Adamek medium under different conditions to improve blastospore production. Mosquito larvae exposed blastospores or conidia three fungal at 1 × 107 propagules mL-1. 153 reduced larval survival by 100%, whereas 489 decreased about 50%. Blastospores had better results lowering survival. similarly. For histopathology (HP) scanning electron...

Here, the effectiveness of Beauveria bassiana LCM S19 and Metarhizium anisopliae S01, combined with Illicium verum essential oil (EO) against Aedes aegypti larvae was investigated. The EO compounds were first identified through gas chromatography, primary compound being (E)-anethole. Subsequently, in silico analysis employed to predict biological activity (E)-anethole, revealing 89 molecular targets, which included insecticidal activity. Next impact on fungal viability colony growth examined...

The use of entomopathogenic fungi to control arthropods has been reported worldwide for decades. aim the present study was evaluate virulence 30 Metarhizium anisopliae s.l. Brazilian isolates from different geographical regions, hosts or substrates on larvae Rhipicephalus (Boophilus) microplus ticks under in vitro conditions selection virulent order be further used biological programs. current confirmed lethal action M. R. (B.) with mortality levels, usually directly proportional conidia...

Brazil has a long history of using biological control and the largest program in sugarcane agriculture to which biocontrol been applied. This achievement is at least partly due utilization entomopathogenic fungus Metarhizium . well-known fungal genus exhibits pathogenicity against broad range arthropod hosts used globally as agent. also root symbiont, this capacity, it plant growth promoter. However, feature (i.e., symbiont) yet be fully explored implemented Brazil, although number reports...

Metarhizium is an entomopathogenic fungus widely employed in the biological control of arthropods. Hemocytes present hemolymph invertebrates are cells involved immune response Despite this, knowledge about Rhipicephalus microplus hemocytes morphological aspects as well their role to fungal infection scarce. The study aimed analyze R. females after robertsii infection, using light and electron microscopy approaches associated with cytotoxicity evaluation. Five types (prohemocytes,...

The aim of the present study was to analyze ten native Metarhizium spp. isolates as their UV-B tolerances. Comparisons included: different fungal propagules (conidia, blastospores, or microsclerotia [MS]); conidia in aqueous suspensions 10% mineral oil-in-water emulsions; and mixed with types soil. effect expressed germination culturability blastospores MS relative nongerminated propagules. anisopliae LCM S05 exhibited high tolerance and/or MS, but not conidia; S10 S08 had positive results...

Abstract Background Ticks are obligate bloodsucking parasites responsible for significant economic losses and concerns with human animal health, mainly due to the transmission of pathogens. Entomopathogenic fungi have been intensively studied as an alternative strategy tick control that can be used in combination synthetic acaricides integrated management ticks. Here, we investigated how gut bacterial community Rhipicephalus microplus is shaped after Metarhizium anisopliae treatment...

The current study evaluates the protein and lipid profile of haemolymph Rhipicephalus (Boophilus) microplus engorged females infected by Metarhizium anisopliae, Beauveria bassiana or Fusarium oxysporum. Ticks were immersed inoculated with conidial suspension. Haemolymph was collected from dorsal surface females. results showed altered total amounts; however, no significant difference observed on electrophoretic among samples. In addition, detected in haemocyte samples ticks treated Metarhizium.

The effect of experimental exposure Biomphalaria glabrata to different doses (5 and 50) Echinostoma paraensei miracidia on the total levels cholesterol triglycerides circulating in hemolymph neutral lipids digestive gland-gonad (DGG) complex were studied. snails dissected one, two, three four weeks after infection collect DGG tissue, measure tissue. results for showed a similar order variation both substrates tested first week infection. reduced these infected indicate intense use by...

The present study aimed to evaluate the pathogenic potential of different Metarhizium anisopliae s.l. isolates and determine whether differences in enzymatic activities proteases, lipases chitinases infection with mycoviruses affect control Rhipicephalus microplus achieved by these fungal isolates. Engorged female ticks were exposed suspensions. lipolytic proteolytic evaluated using chromogenic substrates chitinolytic activity was determined fluorescent substrates. A gel zymography performed...