A5103481483- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Nail Diseases and Treatments
- Infectious Diseases and Mycology
- Mycotoxins in Agriculture and Food
- Fungal Biology and Applications
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Identification and Susceptibility Testing
- HIV/AIDS oral health manifestations
- Ocular Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Infections and bacterial resistance
- Entomopathogenic Microorganisms in Pest Control
- Antibiotics Pharmacokinetics and Efficacy
- Essential Oils and Antimicrobial Activity
- Phytoplasmas and Hemiptera pathogens
- Nematode management and characterization studies
- Cytomegalovirus and herpesvirus research
- T-cell and Retrovirus Studies
- Insect Pest Control Strategies
- Biological Research and Disease Studies
- Fungal Plant Pathogen Control
- Plant-Microbe Interactions and Immunity
- Respiratory and Cough-Related Research
Instituto de Salud Carlos III
2005-2023
Escuela Nacional de Sanidad
2023
Global Fund to Fight AIDS, Tuberculosis and Malaria
2020-2021
University of Manchester
2021
Centro Nacional de Microbiologia
2004-2014
Statens Serum Institut
2012
Innsbruck Medical University
2012
Carnegie Mellon University
2010
Turkish Thoracic Society
2008
Military Medical Academy
2008
We conducted population-based surveillance for Candida bloodstream infections in Spain to determine its incidence, the extent of antifungal resistance, and risk factors mortality. A case was defined as first positive blood culture any spp. a resident Barcelona, from 1 January 2002 31 December 2003. early mortality occurring between days 3 7 after candidemia late 8 30. detected 345 cases candidemia, an average annual incidence 4.3 cases/100,000 population, 0.53 cases/1,000 hospital...
The interaction between fungal pathogens with the host frequently results in morphological changes, such as hyphae formation. encapsulated pathogenic fungus Cryptococcus neoformans is not considered a dimorphic fungus, and predominantly found tissues round yeast cells. However, there specific change associated cryptococcal infection that involves an increase capsule volume. We now report another whereby gigantic cells are formed tissue. paper reports phenotypic characterization of giant...
We have compared the activities of posaconazole and other currently available antifungal agents against a collection 3,378 clinical isolates yeasts filamentous fungi. A total 1,997 Candida spp., 359 yeast species, 697 strains Aspergillus 325 nondermatophyte non-Aspergillus spp. were included. The average geometric means MICs that tested 0.23 microg/ml for amphotericin B, 0.29 flucytosine, 0.97 fluconazole, 0.07 itraconazole, 0.04 voriconazole, 0.15 caspofungin, 0.03 posaconazole....
This study analyzed 28 Aspergillus strains belonging to the section Fumigati that were isolated from clinical samples in Spain. All isolates sporulated slowly and unable grow at 48 degrees C. Phylogenetic analysis based on sequencing of partial sequences beta-tubulin rodlet A genes was used classify into six different clades (Neosartorya hiratsukae, Neosartorya pseudofischeri, viridinutans, lentulus, fumigatiaffinis, fumisynnematus). Antifungal susceptibility testing showed heterogeneous...
Cryptococcus neoformans is a facultative intracellular pathogen. The most distinctive feature of C. polysaccharide capsule that enlarges depending on environmental stimuli. mechanism by which avoids killing during phagocytosis unknown. We hypothesized growth conferred resistance to microbicidal molecules produced the host infection, particularly phagocytosis. observed enlargement reactive oxygen species H(2)O(2) was not associated with higher catalase activity, suggesting new function for as...
Candida parapsilosis has emerged as an important yeast species causing fungemia. We describe the incidence and epidemiology of C. Data from active population-based surveillance in Barcelona, Spain, January 2002 to December 2003 were analyzed. focused on 78 episodes fungemia, we compared them with 175 albicans controls. accounted for 23% all fungemias. The annual incidences 1 episode per 10(5) patients, 1.2 10(4) discharges, 1.7 patient days. All isolates but one (99%) fluconazole...
ABSTRACT A single mechanism of azole resistance was shown to predominate in clinical and environmental Aspergillus fumigatus isolates from the Netherlands, a link use azoles environment suggested. To explore prevalence azole-resistant A. other aspergilli European countries, we collected samples surroundings hospitals Copenhagen, Innsbruck, Madrid, flowerbeds an amusement park compost bags purchased Austria, Denmark, Spain screened for using multidish agars with itraconazole, voriconazole,...
We report the in-vitro susceptibility of 27 clinical isolates Scedosporium apiospermum and 43 prolificans. S. was resistant to fluconazole flucytosine, with variable amphotericin B, itraconazole, ketoconazole susceptible miconazole. Voriconazole much more active than itraconazole as miconazole against isolates. other six antifungal agents showed low activity prolificans
ABSTRACT Antifungal susceptibility testing of molds has been standardized in Europe and the United States. Aspergillus fumigatus strains with resistance to azole drugs have recently detected underlying molecular mechanisms characterized. Three hundred ninety-three isolates, including 32 itraconazole-resistant strains, were used define wild-type populations, epidemiological cutoffs, cross-resistance between drugs. The cutoff for itraconazole, voriconazole, ravuconazole populations A. was ≤1...
The physiological patterns, the sequence polymorphisms of internal transcriber spacer (ITS), and intergenic regions (IGS) rRNA genes antifungal susceptibility profile were evaluated for their ability to identify Trichosporon spp. specificity identification 49 clinical isolates Morphological biochemical methodologies unable differentiate among species. ITS sequencing was also several However, IGS1 unambiguously identified all isolates. Following results DNA-based identification, asahii...
The commercial technique Vitek 2 system for antifungal susceptibility testing of yeast species was evaluated. A collection 154 clinical isolates, including amphotericin B- and azole-resistant organisms, tested. Results were compared with those obtained by the reference procedures both CLSI European Committee on Antimicrobial Susceptibility Testing (EUCAST). Two other techniques approved use, Etest Sensititre YeastOne, included in comparative exercise as well. average essential agreement (EA)...
The zygomycete genus Lichtheimia (syn. Absidia pro parte, Mycocladus) consists of saprotrophic fungi inhabiting soil or dead plant material. corymbifera corymbifera, Mycocladus corymbifer) and ramosa ramosa, ramosus) may cause fulminant infections in patients with impaired immunity. present study investigated the species boundaries using genealogical concordance phylogenetic recognition (by comparison genealogies internal transcribed spacer [ITS] sequence, D1/D2 region large subunit [LSU],...
A two-laboratory study was performed to evaluate the correlation between NCCLS M27-A and EUCAST microdilution procedures for antifungal testing of Candida spp. panel 109 bloodstream isolates tested against amphotericin B, flucytosine, fluconazole, itraconazole. Overall, agreement 92% intraclass coefficient 0.90 (P < 0.05).
Five sequential Cryptococcus neoformans isolates recovered from an AIDS patient with recurrent meningitis were analyzed. Four fluconazole susceptible, while the fifth isolate developed resistance. Analysis of 14-alpha lanosterol demethylase gene (ERG11) showed a point mutation in resistant strain responsible for amino acid substitution G484S.
The antifungal drug susceptibilities of 351 isolates Candida species, obtained through active laboratory-based surveillance in the period January 2002-December 2003, were determined (Candida albicans 51%, parapsilosis 23%, tropicalis 10%, glabrata 9%, krusei 4%).The MICs amphotericin B, flucytosine, fluconazole, itraconazole, voriconazole and caspofungin established by means broth microdilution reference procedure European Committee on Antibiotic Susceptibility Testing.Amphotericin B...
We report on the correlation of outcomes for two cohorts patients who had been treated candidemia (126 episodes) or oropharyngeal candidiasis (110 with various doses fluconazole and MIC obtained by using EUCAST standard fermentative yeasts. Of 145 episodes caused an isolate a < =2 mg/liter, 93.7% (136 145) responded to treatment. The response those infected strain 4 mg/liter was 66% but reached 100% when dose greater than 100 mg/day, whereas strains MICs > =8 only 12%. Hence, 2 able predict...
We have studied infection of Cryptococcus neoformans in the non-vertebrate host Galleria mellonella with particular interest morphological response yeast. Inoculation C. caterpillars induced a capsule-independent increase haemocyte density 2 h after infection. manifested significant capsule size inoculation into caterpillar. The magnitude depended on temperature, being more pronounced at 37°C than 30°C, which correlated an increased virulence fungus and reduced phagocytosis 37°C. Capsule...
A study was designed to assess the reliability of serial detection Aspergillus sp. DNA diagnose invasive aspergillosis (IA) in patients with febrile neutropenia. Two blood and two serum samples were taken weekly from 83 patients. total 2,244 analyzed by real-time quantitative PCR. Twelve (14.4%) diagnosed IA. Taking consecutive positive results as diagnostic criterion, PCR detected 11 cases, 4 false positives, giving sensitivity, specificity, positive, negative predictive values 91.6%,...
ABSTRACT Two methods of inoculum preparation for filamentous fungi were compared: counting with a hematocytometer and spectrophotometric adjustment. One hundred eighty-two pathogenic humans used. Colony counts done all preparations. The agreement between the colony (CFU per milliliter) was 97.2%. reproducibility by means an intraclass correlation coefficient 0.70. Pearson's index versus 0.56, whereas that optical density 0.008. Both can be used size However, use method requires each species...