We report the in-vitro susceptibility of 27 clinical isolates Scedosporium apiospermum and 43 prolificans. S. was resistant to fluconazole flucytosine, with variable amphotericin B, itraconazole, ketoconazole susceptible miconazole. Voriconazole much more active than itraconazole as miconazole against isolates. other six antifungal agents showed low activity prolificans

ABSTRACT The aim of this study was to investigate the basis putative persistence Listeria monocytogenes in a new industrial facility dedicated processing ready-to-eat (RTE) Iberian pork products. Quaternary ammonium compounds, which included benzalkonium chloride (BAC), were repeatedly used as surface disinfectants plant. Clean and disinfected surfaces sampled evaluate if resistance associated with persistence. Of 14 isolates obtained from product contact non-product surfaces, only five...

Contamination routes of Listeria monocytogenes were examined for 3 years in an Iberian pork-processing plant that produced high-quality ready-to-eat meat products. Molecular subtypes L. determined by polymerase chain reaction–based serotyping and pulsed-field gel electrophoresis (PFGE) restriction analysis. A total 541 isolates recovered from the environment equipment (n = 165), carcasses 28), raw products 321), dry-cured 27). Only 29 different PFGE types identified, which repeatedly found...

This study was intended to investigate the effect of contact with concentrations close minimum inhibitory concentration (MIC) (0.5, 1, and 1.5 MIC; MIC planktonic cells determined using a microdilution broth method) sodium hypochlorite (SHY) or benzalkonium chloride (BZK) during process formation biofilm (24 h), upon architecture viability biofilms formed by four L. monocytogenes isolates molecular serotype 1/2a: S2-1 (BZK-susceptible strain; MICBZK = 3.0 ppm), S2-2 (BZK-resistant strain...

Four cases of fatal disseminated Scedosporium prolificans (inflatum) infection occurring in neutropenic patients are reported. Because hospital renovation, the were cared for a temporary hematologic facility. S. was isolated from blood cultures these four patients, two whom underwent full necropsy, and revealed abundant vegetative hyphae ovoid conida with truncate bases many organs. In vitro susceptibility testing fungal strains showed all isolates to be resistant amphotericin B,...

Voriconazole was compared with amphotericin B and itraconazole by a modification of the NCCLS microdilution reference method for yeasts against 62 clinical isolates Aspergillus fumigatus. MICs voriconazole were slightly lower than those itraconazole. The MIC at which 90% inhibited 1 mg/L range 0.25-2 mg/L. is new antifungal agent potential use in treatment A. fumigatus infections.

The National Committee for Clinical Laboratory Standards has proposed a reference broth macrodilution method in vitro antifungal susceptibility testing of yeasts (the M27-P method). This is cumbersome and time-consuming includes MIC endpoint determination by the visual subjective inspection growth inhibition after 48 h incubation. Two alternative microdilution procedures determination, spectrophotometric test that evaluates 80% drug colorimetric with an oxidation-reduction indicator (Alamar...

The National Committee for Clinical Laboratory Standards has proposed a reference broth macrodilution method in vitro antifungal susceptibility testing of yeasts (the M27-P method). This is cumbersome and time-consuming includes MIC endpoint determination by visual subjective inspection growth inhibition after 48 h incubation. An alternative microdilution procedure was compared with the amphotericin B, flucytosine, fluconazole susceptibilities 8 American Type Culture Collection strains (6...

We have compared fluconazole susceptibilities of 92 clinical isolates Candida albicans by broth microdilution in two different media: standard RPMI 1640 (RPMI) and the same medium supplemented with 18 g glucose per liter (RPMI-glucose). Preparation media, drugs, inocula, as well incubation conditions, followed preliminary recommendations National Committee for Clinical Laboratory Standards (Villanova, Pa.) antifungal agent working group macrodilution tests agents, adapted to microdilution....

Listeria monocytogenes is an opportunistic pathogen that widely distributed in the environment. Here we show prevalence and transmission of L. dairy farms Cantabria region, on northern coast Spain. A total 424 samples was collected from 14 (5 organic 9 conventional) 211 isolates were recovered following conventional microbiological methods. There no statistically significant differences antimicrobial resistance ratios between farms. clonal relationship among assessed by pulsed field gel...

Listeriosis is one of the most important food-borne diseases. A variety culture and rapid methods are available for detection Listeria spp. in foods. Although presence L. innocua may indicate potential contamination with monocytogenes, only latter species pathogenic humans. Therefore, adequate tests those which specifically detect monocytogenes. Chromogenic media currently common method used presumptive identification Some like based on antigen fast easily applied, but a few Real-time...

A SYBR Green I based real-time PCR assay with inlA-specific oligonucleotide primers was developed for easy and rapid detection of Listeria monocytogenes in a model food that usually has high incidence contamination this pathogen. Results pure cultures artificially contaminated chicken meat samples indicate the highly specific sensitive. The melting point analysis 160 bp amplified DNA fragment different L. isolates two major phylogenetic divisions species, 1 2. then used to survey retail...

Candida albicans strain delineation by karyotyping. NotI restriction pattern analysis, hybridization with specific probe 27A, and PCR fingerprinting the phage M13 core sequence were performed 30 isolates from oral cavities of human immunodeficiency virus (HIV)-infected patients 8 reference strains. Within panel clinical isolates, 20 geographically related, although 10 susceptible to fluconazole resistant fluconazole. The remaining used in this study unrelated. A composite DNA type was...

We have compared amphotericin B, flucytosine, ketoconazole and fluconazole susceptibilities of 40 clinical isolates Candida albicans by broth microdilution in two different media: RPMI 1640 (RPMI) the same medium supplemented with 18 g glucose per L (RPMI-2% glucose). Preparation media, drugs inocula, as well incubation temperature, followed recommendations National Committee for Clinical Laboratory Standards (Villanova, PA, USA) antifungal agent working group macrodilution tests agents....