A5085844424- Listeria monocytogenes in Food Safety
- Bacterial biofilms and quorum sensing
- Antimicrobial agents and applications
- Essential Oils and Antimicrobial Activity
- Food Safety and Hygiene
- Microbial Inactivation Methods
- Salmonella and Campylobacter epidemiology
- Enterobacteriaceae and Cronobacter Research
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Advanced Chemical Sensor Technologies
- Medical Device Sterilization and Disinfection
- Antimicrobial Peptides and Activities
- Meat and Animal Product Quality
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Parasitic Infections and Diagnostics
- Environmental Chemistry and Analysis
- Microplastics and Plastic Pollution
- Food Quality and Safety Studies
- 3D Printing in Biomedical Research
- Analytical Methods in Pharmaceuticals
- Biosensors and Analytical Detection
- Antibiotic Resistance in Bacteria
Universidad de León
2017-2024
When selecting effective doses of antimicrobials, be they biocides or antibiotics, it is essential to know the minimum inhibitory concentrations (MICs) and bactericidal (MBCs) these substances. The present research determined MICs MBCs for three biocides, sodium hypochlorite (SH), benzalkonium chloride (BC), peracetic acid (PAA), nine antibiotics in eight strains Listeria monocytogenes varying serotypes. Marked intra-species differences were observed resistance L. antibiotics. (ppm) ranged...
The use of subinhibitory concentrations biocides in food processing environments requires special attention because it is related to potential increases antibiotic resistance. In this study, we determined the effect exposure low doses four (sodium hypochlorite, SHY; benzalkonium chloride, BZK; peracetic acid, PAA; trisodium phosphate, TSP) on resistance 10 antibiotics and hydrophobicity cellular surface a strain Listeria monocytogenes serotype 1/2 b (LM) Salmonella enterica Agona (SA), both...
This study was intended to investigate the effect of contact with concentrations close minimum inhibitory concentration (MIC) (0.5, 1, and 1.5 MIC; MIC planktonic cells determined using a microdilution broth method) sodium hypochlorite (SHY) or benzalkonium chloride (BZK) during process formation biofilm (24 h), upon architecture viability biofilms formed by four L. monocytogenes isolates molecular serotype 1/2a: S2-1 (BZK-susceptible strain; MICBZK = 3.0 ppm), S2-2 (BZK-resistant strain...
The susceptibility of Cronobacter sakazakii ATCC 29544 (CS) and Yersinia enterocolitica 9610 (YE) to sodium hypochlorite (10% active chlorine; SHY), peracetic acid (39% solution in acetic acid; PAA) benzalkonium chloride (BZK) was tested. Minimum inhibitory concentration (MIC) values (planktonic cells; microdilution broth method) 3,800 ppm (SHY), 1,200 (PAA) 15 for CS, 2,500 1,275 20 YE, were found. In some instances, an increase growth rate observed presence sub-MICs (0.25MIC, 0.50MIC or...
Some strains of Listeria monocytogenes can persist in food-processing environments, increasing the likelihood contamination foodstuffs. To identify traits that contribute to bacterial persistence, a selection persistent and sporadic L. isolates from poultry-processing facility was investigated for biofilm-forming ability (crystal violet assay). The susceptibility sessile cells treatments (five minutes) with sodium hypochlorite having 10% active chlorine (SHY: 10,000 ppm, 25,000 50,000 ppm)...
A total of 100 samples fresh poultry preparations were obtained from 10 retail outlets in North-Western Spain. Listeria spp. found 73 samples. Isolates identified through polymerase chain reaction (PCR) as monocytogenes (56 samples), innocua (32), grayi (3), seeligeri (1) and (6). In 24 samples, several different species found. The loads L. detected by quantitative (q-PCR) the 56 positive ranged <2.15 log units (limit detection) up to 5.96 units. Neither type sample nor outlet involved had...
Biofilms are a key factor in the persistence of Listeria food processing plants, representing potential source foodstuff contamination. Nine strains (eight monocytogenes and one ivanovii) were studied by confocal laser scanning microscopy (CLSM) for their ability to form biofilm on glass, polystyrene, graphene resin after 120 h incubation at 12 °C. The relationship between cell surface hydrophobicity formation was also investigated. On comparing data all strains, similar (P > 0.05)...
A total of 44 samples beef, pork, and poultry preparations were tested. Average counts (log cfu/g) enterobacteria 1.99 ± 0.99 (beef preparations), 1.96 1.44 (pork), 2.09 0.92 (chicken), 2.17 1.06 (turkey) (p > 0.05). Two hundred enterobacterial strains identified 13 genera (21 species) distinguished, including species that are a significant cause infection. The most common Escherichia (32.5% strains), Serratia (17.0%), Hafnia (12.5%), Salmonella (12.0%). Isolates screened by disc...
Knowledge of the microbiota present in food processing environments is a significant advance that will allow for better evaluation risk contamination and design procedures sanitization. The levels microbial group indicators hygienic quality were determined different areas slaughter lines two poultry slaughterhouses northwest Spain (22 surfaces each slaughterhouse studied). average (cfu/cm2) 2.15 × 102 ± 4.26 (total aerobic counts, TAC), 1.99 5.00 (psychrotrophic microorganisms), 3.10 100...
Abstract According to the World Health Organization, contaminated food causes approximately 600 million cases of illness and 420,000 associated deaths worldwide each year. In this scenario, safety is a crucial task, which based in application correct hygiene practices throughout entire production chain: from farm table. Ensuring requires implementation practical, comprehensive, affordable, effective approaches such as Hazard Analysis Critical Control Points, Good Agricultural Practices,...
Twenty samples of minced chicken meat procured from butcher’s shops in León (Spain; 10 samples) and Vila Real (Portugal; were analyzed. Microbial concentrations (log10 cfu/g) 7.53 ± 1.02 (viable aerobic microbiota), 7.13 1.07 (psychrotrophic microorganisms), 4.23 0.88 (enterobacteria) found. The detection method described the UNE-EN ISO 11290-1 standard (based on isolation chromogenic medium OCLA) with confirmation by polymerase chain reaction (PCR; lmo1030) (OCLA−PCR), revealed Listeria...
In certain circumstances, disinfectants are used at sublethal concentrations. The aim of this research work was to determine whether contact Listeria monocytogenes NCTC 11994 with subinhibitory concentrations three widely in food processing environments and the health-care system, benzalkonium chloride (BZK), sodium hypochlorite (SHY) peracetic acid (PAA), can cause adaptation strain biocides increase its resistance tetracycline (TE). minimum inhibitory (MIC; ppm) were 2.0 3500.0 1050.0...
The impact of treating minced chicken meat with sodium nitrite (SN, 100 ppm), nisin (Ni, 10 ppm) and lactic acid (LA, 3000 on the levels some microbial groups indicating hygiene quality were investigated. Specifically, aerobic plate counts culture-based psychrotrophic microorganisms enterobacteria obtained. Additionally, prevalence Listeria monocytogenes resistance 245 isolates from this bacterium to 15 antibiotics documented. L. was isolated using ISO 11290-1:2017 method confirmed...