Polycationic peptides may present their C-termini in either amidated or acidic form; however, the effects of these conformations on mechanisms interaction with membranes general were not properly investigated up to now. Protonectarina-MP mastoparan an C-terminus was utilized study interactions anionic and zwitterionic vesicles, using measurements dye leakage a combination H/D exchange mass spectrometry monitor peptide–membrane interactions. Mast cell degranulation, hemolysis antibiosis...

The occurrence and spatial distribution of the neuropeptides AmTRP-5 AST-1 in honeybee brain were monitored via MALDI spectral imaging according to ontogeny Africanized Apis mellifera. levels these peptides increased brains 0–15 day old honeybees, this increase was accompanied by an number in-hive activities performed nurse bees, followed a decrease period from 15 25 days age, which workers began perform outside nest (guarding foraging). results obtained present investigation suggest that...

Invasive fungal infections such as cryptococcosis and paracoccidioidomycosis are associated with significant rates of morbidity mortality. Specially the caused by Cryptococcus neoformans, is distributed worldwide, has received much attention a common complication in patients HIV. usually treated combination amphotericin B azoles. In addition, 5-fluorocytosine (5-FC) applied cryptococcosis, especially to treat central nervous system infection. However, host toxicity, high cost, emerging...

Abstract Previous studies of the fractionated venom Brazilian armed spider Phoneutria nigriventer , obtained by gel filtration, have demonstrated presence a fraction PhM, pool small peptides (up to 2000 Da) that provoke contractions in smooth muscle guinea pig ileum. Initial attempts sequence these were largely unsuccessful because low purification yield and fact majority seemed be blocked at their N‐termini. In present work, analysis this mass spectrometry has revealed existence highly...

In this study, a series of mastoparan analogs were engineered based on the strategies Ala and Lys scanning in relation to sequences classical mastoparans. Ten analog mastoparans, presenting from zero six residues their synthesized assayed for some typical biological activities group peptide: mast cell degranulation, hemolysis, antibiosis. apparent structural requirement optimize activity was existence one or two at positions 8 and/or 9. feature that strongly correlated with potency number...

Most crude venom from Polybia paulista is composed of short, linear peptides; however, only five these peptides are structurally and functionally characterized. Therefore, the in this were profiled using an HPLC-IT-TOF/MS MSn system. The presence type -d -w ions that generated fragmentation side chains was used to resolve I/L ambiguity. distinction between K Q residues achieved through esterification α- ε-amino groups peptide chains, followed by mass spectrometry analysis. Fourteen major...

Aim: Gallic acid and its ester derivatives have shown antifungal activity in vitro. This study was performed to investigate their against Candida albicans toxicity the animal models Caenorhabditis elegans zebrafish embryos. Results: The compounds protected worms from C. infection. dodecyl gallate most effective. In embryo, gallic were least toxic. Conclusion: potential for vivo use effects of esters these alternative dependent on carbon chain length.

Enteral nutrition therapy (ENT) is an essential part in the management of critically ill patients, having a significant impact on these patients' clinical results. It can be administered continuous or intermittent basis using infusion pump. There discussion which techniques has best performance, involving number factors such as nausea, diarrhea, and particularly relationship between diet volume ratio programed calories to effectively supplied critical patients.To compare forms enteral...

Aim: This work aimed to evaluate the activity of 3′-hydroxychalcone against Cryptococcus gattii in planktonic and biofilm forms their toxicity using alternative animal models. Materials & methods: Minimum inhibitory concentration minimum fungicide were determined. Biofilm formation susceptibility tests performed by 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-5-[carbonyl(phenylamino)]-2H-tetrazolium hydroxide assay. Toxicity efficacy checked Danio rerio Galleria mellonella Results: The compound...

The venoms of the social wasps evolved to be used as defensive tools protect colonies these insects against attacks predators. Previous studies estimated presence a dozen peptide components in each species insects, which altogether comprise up 70% weight freeze-dried venoms. In present study, an optimized experimental protocol is reported that utilizes liquid chromatography coupled electrospray ionization mass spectrometry for detection peptides venom wasp Polybia paulista; profiles both...

Honeybee stings are a severe public health problem. Bee venom contains series of active components, including enzymes, peptides, and biogenic amines. The local reactions observed after envenoming include typical inflammatory response pain. some well-known polycationic such as Melittin, Apamin, MCD peptide, Cardiopep, Tertiapin. Secapin in honeybee was described 38 years ago, yet almost nothing is known about its action. A novel, variant form this peptide isolated from the Africanized...

Colonial spiders evolved a differential prey-capture behaviour in concert with their venom chemistry, which may be source of novel drugs. Some highly active tetrahydro-β-carboline (THβC) toxins were recently isolated from the colonial spider Parawixia bistriata; use these as part chemical arsenal to kill and/or paralyze preys. The major THβC compound this was identified 6-hydroxytrypargine, also known PwTX-I. Most natural compounds animal origin occur low abundance, and abundance PwTX-I is...

Objetivo: Refletir sobre a consulta de enfermagem como ferramenta cuidado integral na Atencao Primaria Saude, contemplando marcos historicos e praticos que envolve tematica. Metodo: Trata-se uma revisao narrativa, desenvolvida por meio aspectos conceituais advindos livros artigos cientificos, filtrados em bases portais como: BVS, Lilacs, Medline, Scielo, alem documentos legais. Resultados: Foram elencados tres eixos teoricos norteadores: O cuidar da enfermagem: historicos; A pratica Consulta...

ABSTRACT BACKGROUND Abbreviation of preoperative fasting to 2 hours with maltodextrin (CHO)-enriched beverage is a safe procedure and may enhance postoperative recovery. Addition glutamine (GLN) CHO beverages include potential benefits the metabolism. However, by adding nitrogenous source beverages, gastric emptying be delayed increase risk bronchoaspiration during anesthesia. OBJECTIVE In this study safety, we aimed at investigating residual volume (RGV) after intake either alone or...

The peptide Paulistine was isolated from the venom of wasp Polybia paulista. This exists under a natural equilibrium between forms: oxidised - with an intra-molecular disulphide bridge; and reduced in which thiol groups cysteine residues do not form bridge. biological activities both forms are unknown up to now.Both were synthesised protected acetamidemethyl group [Acm-Paulistine] prevent re-oxidation. structure/activity relationships two investigated, taking into account importance...

Nigriventrine was isolated from the "armed" spider Phoneutria nigriventer, in which it constitutes about 0.4% of total venom content. Its structure determined to be [1,1′-(1-hydroxyhydrazine-1,2-diyl)bis(oxy)bis(4-hydroxy-2,6-dioxopiperidine-4 carboxylic acid)] by NMR, HR-ES/IMS and MS/MS methods. The intracerebroventricular application nigriventrine rat brain, followed detection c-Fos protein expression, indicated that compound neuroactive motor cortex, sensory piriform median preoptic...

Cryptococcosis is associated with high rates of morbidity and mortality, especially in AIDS patients. Its treatment carried out by combining amphotericin B azoles or flucytosine, which causes unavoidable toxicity issues the host.

Aim: In this work, mastoparan analog peptides from wasp venom were tested against Candida albicans and safety assays performed using cell culture model zebrafish. Materials & methods: Minimal inhibitory concentration was determined toxicity human skin keratinocyte embryo Also, permeation of through chorion performed. Results: The demonstrated anti-C. activity, with low cytotoxicity nonteratogenicity in Danio rerio. compounds had different chorion, suggesting that occurs due to modifications...

Lors de l'hiver 2006, l'association Les Enfants Don Quichotte installe un campement tentes sur les berges du canal Saint-Martin, à Paris. Il s'agit alors susciter le débat public au sujet la situation des SDF, question qui semble confinée dans l'angle mort tant point vue politiques publiques, que celui usages ordinaires constitutifs espaces publics. L'originalité l'action entreprise tient sa mise en scène, est structurée par scénario, c'est-à-dire une récit opérée et l'espace.

The digestibility and true digestible amino acid content of eight cultivars maize was determined using the method force-feeding roosters cecectomized. experimental design completely randomized, represented by cultivars, five replicates a rooster each. Five were kept fasting for corrections corresponding to metabolic endogenous losses acids. Significant differences (P <0.05) among acids, methionine, threonine, arginine, cystine serine observed. values coefficients essential threonine...