A5072102197- Venomous Animal Envenomation and Studies
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Antimicrobial Peptides and Activities
- Healthcare and Venom Research
- Insect and Pesticide Research
- Neurobiology and Insect Physiology Research
- Biochemical and Structural Characterization
- Diphtheria, Corynebacterium, and Tetanus
- Marine Sponges and Natural Products
- Research on Leishmaniasis Studies
- Pain Mechanisms and Treatments
- Marine Invertebrate Physiology and Ecology
- Sexual function and dysfunction studies
- Streptococcal Infections and Treatments
- Toxin Mechanisms and Immunotoxins
- Hormonal and reproductive studies
- Bioactive Compounds and Antitumor Agents
- Rabies epidemiology and control
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Beetle Biology and Toxicology Studies
- Bacterial Identification and Susceptibility Testing
- Heavy Metals in Plants
- Neuropeptides and Animal Physiology
Universidade Federal de Minas Gerais
2015-2025
Jewish General Hospital
2025
McGill University
2025
Associação Brasileira de Alergia e Imunologia
2022
University of Southern Denmark
2013-2020
Pontifícia Universidade Católica de Minas Gerais
2020
Centre National de la Recherche Scientifique
2001-2020
Ezequiel Dias Foundation
2020
Instituto de Ensino e Pesquisa Santa Casa
2020
Universidade Federal dos Vales do Jequitinhonha e Mucuri
2020
The renin-angiotensin system (RAS) is a key regulator of the cardiovascular system, electrolyte, and water balance. Here, we report identification characterization alamandine, new heptapeptide generated by catalytic action angiotensin-converting enzyme-2 angiotensin A or directly from angiotensin-(1-7).To characterize novel component RAS, alamandine.Using mass spectrometry observed that alamandine circulates in human blood can be formed angiotensin-(1-7) heart. Alamandine produces several...
This single-volume edition presents for the first time results of several studies with different perspectives on real possibilities use animal venoms and toxins in biotechnology industry.Animal Toxins consists 39 articles, signed by renowned experts various nationalities.The state art compounds derived from marine animals, spiders scorpions, lizards, snakes, among others, are focus this publication which aims to meet scientists, students university researchers, biotechnologists interested...
Abstract Scorpion venoms are very complex mixtures of molecules, most which peptides that display different kinds biological activity. These have been studied in the light their pharmacological targets and constituents able to bind specifically a variety ionic channels located prey tissues, resulting neurotoxic effects. Toxins modulate Na + , K Ca ++ Cl − currents described scorpion venoms. Mass spectrometry was employed analyze toxic fractions from venom Brazilian Tityus serrulatus order...
Abstract Background Bacterial exported proteins represent key components of the host-pathogen interplay. Hence, we sought to implement a combined approach for characterizing entire exoproteome pathogenic bacterium Corynebacterium pseudotuberculosis , etiological agent caseous lymphadenitis (CLA) in sheep and goats. Results An optimized protocol three-phase partitioning (TPP) was used obtain C. exoproteins, newly introduced method data-independent MS acquisition (LC-MS E ) employed protein...
Background: Quantifying urinary catecholamines and metanephrines is essential for the clinical screening diagnosis of neuroendocrine tumours. HPLC with electrochemical detection (HPLC-ECD) commonly used this type analysis but requires extensive sample cleanup. Simple rapid dilute-and-shoot LC-multiple-reaction monitoring (MRM)-MS assays have been developed quantitating these analytes in urine not yet validated according to Clinical Laboratory Standards Institute (CLSI) guidelines. Methods: A...
Bioactive peptides are promising therapeutic agents due to their antimicrobial and anticancer activities, although lack of selectivity often limits clinical applications. This study demonstrates the optimal synthetic route for conjugating folic acid (FA) with bioactive peptide Lunatin-1, aiming improve neoplastic cells. The synthesis combines solid-phase (SPPS) Cu(I)-catalyzed cycloaddition link Lunatin-1 via a triazole ring. Using model tripeptide FIG-NH2, key intermediates final product...
Besides being a public health problem, scorpion venoms have potential biotechnological application since they contain peptides that may be used as drug leads and/or to reveal novel pharmacological targets. A comprehensive Tityus serrulatus venom proteome study with emphasis on the phosphoproteome and N-glycoproteome was performed improve our knowledge molecular diversity of proteinaceous toxins. We combined two peptide identification methodologies, i.e., database search de novo sequencing,...
Abstract Venom variability in specimens of Tityus serrulatus scorpion was assessed by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) analyses. An expanded time lag venom extraction protocol carried out using ten scorpions to study individual variations that might occur due different rates protein expression and/or processing. The first made from the animals after 20 days starvation, which allowed gland be filled up. second event 24 hours one. third...
In order to better understand the relationship between primary structure of TsHpt-I - a bradykinin-potentiating peptide (BPP) isolated from venom yellow scorpion Tityus serrulatus, with non-canonical Lys residue prior conservative Pro-Pro doublet and its cardiovascular effects, series ladder peptides were synthesized using C-terminal portion as template. All synthetic having at their able potentiate hypotensive effect bradykinin. Conversely, only those analogues could induce transient...
Sea anemones are known to contain a wide diversity of biologically active peptides, mostly unexplored according recent peptidomic and transcriptomic studies. In the present work, neurotoxic fractions from exudates Stichodactyla helianthus Bunodosoma granulifera were analyzed by reversed-phase chromatography mass spectrometry. The first peptide fingerprints these sea assessed, revealing largest number components (156) so far found in anemone species, as well richer B. relation S. helianthus....