A5009364832- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Botulinum Toxin and Related Neurological Disorders
- Adenosine and Purinergic Signaling
- Ion Channels and Receptors
- Neuroscience and Neuropharmacology Research
- Nerve injury and regeneration
- Pharmacological Effects of Natural Compounds
- Geomagnetism and Paleomagnetism Studies
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience of respiration and sleep
- Inflammatory mediators and NSAID effects
- Solar and Space Plasma Dynamics
- Laser Applications in Dentistry and Medicine
- Stress Responses and Cortisol
- Anesthesia and Pain Management
- Pain Management and Placebo Effect
- Virus-based gene therapy research
- Urticaria and Related Conditions
- Neurobiology and Insect Physiology Research
- Temporomandibular Joint Disorders
- Bee Products Chemical Analysis
- Myofascial pain diagnosis and treatment
Universidade Estadual de Campinas (UNICAMP)
2015-2024
University of California, San Diego
2022
Hospital de Clínicas da Unicamp
2021
Universidade de São Paulo
2003-2015
Universidade de Ribeirão Preto
2004-2010
University of Wisconsin–Madison
2007
University of California, San Francisco
2002-2005
Vall d'Hebron Hospital Universitari
2004
Centro Nacional de Microbiologia
2003
Hospital Universitario Puerta de Hierro Majadahonda
2001-2003
The aim of the present investigation was to describe and validate an electronic mechanical test for quantification intensity inflammatory nociception in mice. pressure-meter consists inducing animal hindpaw flexion reflex by poking plantar region with a polypropylene pipette tip adapted hand-held force transducer. This method compared classical von Frey filaments which pressure is automatically recorded after nociceptive reflex. were used detect time versus treatment interactions...
Abstract Neutrophil migration is responsible for tissue damage observed in inflammatory diseases. Neutrophils are also implicated nociception, but mechanisms of their participation have not been elucidated. In the present study, we addressed these carrageenan-induced mechanical hypernociception, which was determined using a modification Randall-Sellito test rats. accumulation into plantar by contents myeloperoxidase activity, whereas cytokines and PGE2 levels were measured ELISA...
The development of treatments for neuropathic pain has been hindered by our limited understanding the basic mechanisms underlying abnormalities in nociceptor hyperexcitability. We recently showed that polymodal receptor transient potential vanilloid 4 (TRPV4), a member (TRP) family ion channels, may play role inflammatory (Alessandri-Haber et al., 2003). present study tested whether TRVP4 also contributes to pain, using rat model Taxol-induced painful peripheral neuropathy. Taxol is most...
The objective of the present investigation was to compare sensitivity an electronic nociceptive mechanical paw test with classical tests quantify intensity variation inflammatory nociception. pressure-meter consists inducing hindpaw flexion reflex by poking plantar region a polypropylene pipette tip adapted hand-held force transducer. This method compared von Frey filaments and rat constant pressure test, modification Randall Selitto developed our group. When comparing three methods, but not...
Herpes simplex virus type 1 (HSV-1) mutants that fail to express the viral immediate-early protein ICP0 have a pronounced defect in gene expression and plaque formation limited-passage human fibroblasts. is RING finger E3 ubiquitin ligase induces degradation of several cellular proteins. PML, organizer nuclear substructures known as PML bodies or ND10, one most notable proteins targeted by ICP0. Depletion from fibroblasts increases ICP0-null mutant HSV-1 expression, but not wild-type levels....
Temporomandibular joint (TMJ) pain conditions are poorly understood. Since formalin is a noxious stimulus widely used in animal behavioral experiments for studying mechanisms, the aim of this study was to develop model TMJ by characterizing nociceptive responses induced injection into region rats. NaCl (0.9%) or different concentrations (0.5, 1.5, 2.5 5%) were administrated region. The formalin-induced characterized moving mandible, rubbing orofacial and flinching head quickly quantified 45...
Abstract Carrageenan‐induced inflammatory pain lasting hours to days produces a protein kinase C epsilon (PKCɛ)‐dependent ‘primed’ state several weeks, during which time injection of prostaglandin E 2 induces hyperalgesia is markedly enhanced and prolonged compared PGE ‐induced in normal ‘unprimed’ rats. In the present study, we demonstrate that while inhibition synthesis antagonism β ‐adrenergic receptors attenuated induced by carrageenan, these interventions did not affect hyperalgesic...
Toward the goal of defining new pharmacological targets for treatment chronic pain conditions, in previous studies we established a model, termed 'hyperalgesic priming,' which an acute inflammatory stimulus causes long-lasting latent susceptibility to hyperalgesia induced by subsequent exposures mediator, prostaglandin E2 (PGE2). Those investigations suggested hypothesis that priming induces novel linkage between PGE2-activated second messenger cascade and epsilon isoform protein kinase C...
Abstract Leukotriene B4 (LTB4) mediates different inflammatory events such as neutrophil migration and pain. The present study addressed the mechanisms of LTB4-mediated joint inflammation-induced hypernociception. It was observed that zymosan-induced articular hypernociception were reduced dose-dependently by pretreatment with MK886 (1–9 mg/kg; LT synthesis inhibitor) well in 5-lypoxygenase-deficient mice (5LO−/−) or selective antagonist LTB4 receptor (CP105696; 3 mg/kg). Histological...
Through activation of the A1 adenosine receptors (A1Rs) at both central and peripheral level, produces antinociception in a wide range tests. However, mechanisms involved effect are still not fully understood. Therefore, by which A1Rs reduces inflammatory hypernociception (a decrease nociceptive threshold) were addressed present study. Immunofluorescence rat dorsal root ganglion revealed significant expression primary sensory neurons associated with pathways. Functionally, reduced because...
Abstract Many painful conditions are associated with alterations in the extracellular matrix (ECM) of affected tissues. While several integrins, receptors for ECM proteins, present on sensory neurons that mediate pain, possible role these cell adhesion molecules inflammatory or neuropathic pain has not been explored. We found intradermal injection peptide fragments domains laminin and fibronectin important adhesive signaling selectively inhibited hyperalgesia caused by prostaglandin E 2 (PGE...
Activation of P2X3,2/3 receptors by endogenous ATP contributes to the development inflammatory hyperalgesia. Given clinical importance mechanical hyperalgesia in states, we hypothesized that activation carrageenan-induced and this contribution is mediated an indirect and/or a direct sensitization primary afferent nociceptors. Co-administration selective antagonist A-317491, or non-selective P2X3 receptor antagonist, TNP-ATP, with carrageenan blocked induced carrageenan, significantly reduced...
The present study evaluated the role of N-methyl-D-aspartate receptors (NMDARs) expressed in dorsal root ganglia (DRG) inflammatory sensitization peripheral nociceptor terminals to mechanical stimulation. Injection NMDA into fifth lumbar (L5)-DRG induced hyperalgesia rat hind paw with a profile similar that intraplantar injection prostaglandin E2 (PGE2), which was significantly attenuated by NMDAR antagonist D(-)-2-amino-5-phosphonopentanoic acid (D-AP-5) L5-DRG. Moreover, blockade DRG AMPA...
It is well established that dorsal root ganglion (DRG) cells synthesize prostaglandin. However, the role prostaglandin plays in inflammatory hyperalgesia of peripheral tissue has not been established. Recently, we have successfully a technique to inject drugs (3 μL) directly into L5-DRG rats, allowing vivo identification DRG cell-derived COX-1 and COX-2 play development tissue. IL-1β (0.5 pg) or carrageenan (100 ng) was administered L5-peripheral field rat hindpaw mechanical evaluated after...
Dipyrone (metamizole) is an analgesic pro-drug used to control moderate pain. It metabolized in two major bioactive metabolites: 4-methylaminoantipyrine (4-MAA) and 4-aminoantipyrine (4-AA). The aim of this study was investigate the participation peripheral CB1 CB2 cannabinoid receptors activation anti-hyperalgesic effect dipyrone, 4-MAA or 4-AA. PGE2 (100ng/50µL/paw) locally administered hindpaw male Wistar rats, mechanical nociceptive threshold quantified by electronic von Frey test,...