A5036423490- Pain Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Neuroinflammation and Neurodegeneration Mechanisms
- Glioma Diagnosis and Treatment
- Calpain Protease Function and Regulation
- Neuropeptides and Animal Physiology
- Diabetic Foot Ulcer Assessment and Management
- Extracellular vesicles in disease
- Receptor Mechanisms and Signaling
- Neurogenesis and neuroplasticity mechanisms
- Epilepsy research and treatment
- Mitochondrial Function and Pathology
- Nerve injury and regeneration
- Functional Brain Connectivity Studies
- Single-cell and spatial transcriptomics
- Ion channel regulation and function
- Bipolar Disorder and Treatment
- Botulinum Toxin and Related Neurological Disorders
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Adenosine and Purinergic Signaling
- Pharmacological Effects of Natural Compounds
- Neural dynamics and brain function
- Genomics and Rare Diseases
- Electroconvulsive Therapy Studies
Brazilian Center for Research in Energy and Materials
2024
Universidade Estadual de Campinas (UNICAMP)
2013-2023
Brazilian Institute of Neuroscience and Neurotechnology
2019-2023
Instituto Nacional de Psiquiatría
2012
Peripheral nerve lesions provoke apoptosis in the dorsal horn of spinal cord. The cause cell death, involvement neurons, and relevance for processing somatosensory information are controversial. Here, we demonstrate a mouse model sciatic injury that glutamate-induced neurodegeneration loss γ-aminobutyric acid (GABA)ergic interneurons superficial promote transition from acute to chronic neuropathic pain. Conditional deletion Grin1, essential subunit N-methyl-d-aspartate-type glutamate...
The study of brain connectivity has been a cornerstone in understanding the complexities neurological and psychiatric disorders. It provided invaluable insights into functional architecture how it is perturbed However, persistent challenge achieving proper spatial resolution, developing computational algorithms to address biological questions at multi-cellular level, scale often referred as mesoscale. Historically, neuroimaging studies have predominantly focused on macroscale, providing...
It is well established that dorsal root ganglion (DRG) cells synthesize prostaglandin. However, the role prostaglandin plays in inflammatory hyperalgesia of peripheral tissue has not been established. Recently, we have successfully a technique to inject drugs (3 μL) directly into L5-DRG rats, allowing vivo identification DRG cell-derived COX-1 and COX-2 play development tissue. IL-1β (0.5 pg) or carrageenan (100 ng) was administered L5-peripheral field rat hindpaw mechanical evaluated after...
Dipyrone (metamizole) is an analgesic pro-drug used to control moderate pain. It metabolized in two major bioactive metabolites: 4-methylaminoantipyrine (4-MAA) and 4-aminoantipyrine (4-AA). The aim of this study was investigate the participation peripheral CB1 CB2 cannabinoid receptors activation anti-hyperalgesic effect dipyrone, 4-MAA or 4-AA. PGE2 (100ng/50µL/paw) locally administered hindpaw male Wistar rats, mechanical nociceptive threshold quantified by electronic von Frey test,...
Introduction Focal cortical dysplasia (FCD) is a common cause of pharmacoresistant epilepsy. According to the 2022 International League Against Epilepsy classification, FCD type II characterized by dysmorphic neurons (IIa and IIb) may be associated with balloon cells (IIb). We present multicentric study evaluate transcriptomes gray white matters surgical specimens. aimed contribute pathophysiology tissue characterization. Methods investigated (a b) control samples performing RNA-sequencing...
Dopaminergic neurotransmission in the nucleus accumbens, a central component of mesolimbic system, has been associated with acute pain modulation. As there is transition from to chronic ('chronification'), modulatory structures may be involved development. Thus, this study aimed elucidate role accumbens dopaminergic chronification pain. We used rat model which daily subcutaneous injection prostaglandin E2 hindpaw for 14 days induces long-lasting state nociceptor sensitization that lasts at...
Abstract Focal cortical dysplasia is a highly epileptogenic malformation with few treatment options. Here, we generated human organoids from patients focal type II. Using this model, mimicked some hallmarks, such as impaired cell proliferation, the presence of dysmorphic neurons and balloon cells, neuronal network hyperexcitability. Furthermore, observed alterations in adherens junctions zonula occludens-1 partitioning defective 3, reduced polarization actin cytoskeleton, fewer synaptic...
Focal cortical dysplasia (FCD) is a brain malformation that causes medically refractory epilepsy. FCD classified into three categories based on structural and cellular abnormalities, with type II being the most common characterized by disrupted organization of cortex abnormal neuronal development. In this study, we employed cell-type deconvolution single-cell signatures to analyze bulk RNA-seq from multiple transcriptomic studies, aiming characterize composition lesions in patients IIa IIb...
The pilocarpine-induced (PILO) model has helped elucidate the electrophysiological and molecular aspects related to mesial temporal lobe epilepsy. It been suggested that extensive cell death edema observed in brains of these animals could be induced by increased inflammatory responses, such as rapid release cytokine interleukin 1 beta (Il1b). In this study, we investigate role endogenous Il1b acute phase PILO model. Our aim is twofold. First, want determine whether it feasible silence...
Abstract Preconditioning is a mechanism in which injuries induced by non‐lethal hypoxia or seizures trigger cellular resistance to subsequent events. Norwood et al., 2010 study, showed that an 8‐h‐long period of electrical stimulation the perforant pathway rats required for induction hippocampal sclerosis. However, order avoid generalized seizures, status epilepticus (SE), and death, state must be hippocampus preconditioning paradigm consisting two daily 30‐min periods. Due importance...
ABSTRACT Focal cortical dysplasia (FCD) is a common cause of pharmacoresistant epilepsy. According to the 2022 International League Against Epilepsy classification, FCD type II characterized by dysmorphic neurons (IIa and IIb) may be associated with balloon cells (IIb). We present multicentric study evaluate transcriptomes gray white matters surgical specimens. aimed contribute pathophysiology tissue characterization. investigated (a b) control samples performing RNA-sequencing followed...
Abstract Focal cortical dysplasia (FCD) is a brain malformation that causes medically refractory epilepsy. FCD classified into three categories based on structural and cellular abnormalities, with type II being the most common characterized by disrupted organization of cortex abnormal neuronal development. In this study, we employed cell-type deconvolution single-cell signatures to analyze bulk RNA-seq from multiple transcriptomic studies, aiming characterize composition lesions in patients...
Abstract Preconditioning is a mechanism in which injuries induced by non-lethal hypoxia or seizures trigger cellular resistance to subsequent events. Norwood et al., 2010 study, showed that an 8-hour-long period of electrical stimulation the perforant pathway rats required for induction hippocampal sclerosis. However, order avoid generalized seizures, status epilepticus (SE), and death, state must be hippocampus preconditioning paradigm consisting 2 daily 30-minute periods. Due importance...
ABSTRACT The hippocampus comprises several neuronal populations such as CA1, CA2, CA3, and the dentate gyrus (DG), which present different origins, morphologies, molecular mechanisms. Laser capture microdissection (LCM) allows selectively collecting samples from target regions eliminating unwanted cells to obtain more specific results. LCM of coupled with RNA-seq analysis has potential allow exploration machinery unique each these subfields. Previous investigation already provided a...