A5040639046- RNA Interference and Gene Delivery
- Tardigrade Biology and Ecology
- CRISPR and Genetic Engineering
- Spaceflight effects on biology
- MicroRNA in disease regulation
- Sugarcane Cultivation and Processing
- Science and Education Research
- Biocrusts and Microbial Ecology
- Advanced biosensing and bioanalysis techniques
- Trace Elements in Health
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- RNA Research and Splicing
- Viral Infections and Immunology Research
- Genetics, Bioinformatics, and Biomedical Research
- Cancer-related molecular mechanisms research
- Biofuel production and bioconversion
- Prion Diseases and Protein Misfolding
- Plant tissue culture and regeneration
- Parasites and Host Interactions
- Rural and Ethnic Education
- Animal Genetics and Reproduction
- Circular RNAs in diseases
- Virus-based gene therapy research
- Genetic Neurodegenerative Diseases
Universidade de São Paulo
2015-2024
Universidade de Ribeirão Preto
2013-2024
Universidade Estadual de Campinas (UNICAMP)
2005-2022
Brazilian Institute of Neuroscience and Neurotechnology
2022
Seevic College
2020
Universidade Federal de Mato Grosso
2018
University of Cambridge
2010
Hypertrophy is a critical event in the onset of failure chronically overloaded hearts. Focal adhesion kinase (FAK) has attracted particular attention as mediator hypertrophy induced by increased load. Here, we demonstrate expression and phosphorylation FAK hypertrophic left ventricles (LVs) aortic-banded mice. We used an RNA interference strategy to examine whether signaling plays role pathophysiology load-induced LV failure. Intrajugular delivery specific small interfering prolonged...
Abstract Background Some organisms can survive extreme desiccation by entering a state of suspended animation known as anhydrobiosis. The free-living mycophagous nematode Aphelenchus avenae be induced to enter anhydrobiosis pre-exposure moderate reductions in relative humidity (RH) prior desiccation. This preconditioning phase is thought allow modification the transcriptome activation genes required for tolerance. Results To identify such genes, panel expressed sequence tags (ESTs) enriched...
The molecular basis of anhydrobiosis, the state suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number transcriptome and proteome studies. We therefore conducted functional screening RNA interference (RNAi) for genes involved in anhydrobiosis holo-anhydrobiotic nematode Panagrolaimus superbus. A new method survival analysis, based on staining, proof-of-principle RNAi experiments confirmed role oxidative stress tolerance, while...
Abstract Drought is the most detrimental abiotic stress to sugarcane production. Nevertheless, transcriptomic analyses remain scarce for field-grown plants. Here we performed comparative transcriptional profiling of two contrasting genotypes, ‘IACSP97-7065’ (drought-sensitive) and ‘IACSP94-2094’ (drought-tolerant) grown in a drought-prone environment. Physiological parameters expression profiles were analyzed at 42 (May) 117 (August) days after last rainfall. The first sampling was done...
Drought is a major constraint to sugarcane (Saccharum spp.) production and improving the water use efficiency (WUE) critical trait for sustainability of this bioenergy crop. The molecular mechanism underlying WUE remains underexplored in sugarcane. Here, we investigated drought-triggered physiological transcriptional responses two cultivars contrasting drought tolerance, 'IACSP97-7065' (sensitive) 'IACSP94-2094' (tolerant). After 21 days without irrigation (DWI), only exhibited superior...
Background Herpetic encephalitis (HSE) is caused mainly by herpes simplex virus type 1 (HSV-1) with an annual incidence of 1–4 cases/million inhabitants. Currently, HSE treatment faces difficulties such as the use antivirals elevated toxicity, metabolic side effects and HSV-1 resistance. An alternative to small interfering RNA (siRNA) a viral replication inhibitor. In this work, siRNA targeting UL-39 region was evaluated for in vivo. Methods BALB/c mice were inoculated treated siRNA. The...
Machado-Joseph disease (MJD) or spinocerebellar ataxia type 3 (SCA3) is an autosomal dominant neurodegenerative disorder caused by expansion of the polyglutamine domain ataxin-3 (ATX3) protein. MJD/SCA3 most frequent in many countries. The mechanism underlying thought to be mainly related protein misfolding and aggregation leading neuronal dysfunction followed cell death. Currently, there are no effective treatments for patients with MJD/SCA3. Here, we report on potential use lithium...
Spontaneous parthenogenetic and androgenetic events occur in humans, but they result tumours: the ovarian teratoma hydatidiform mole, respectively. However, observation of fetiform (ovarian) teratomas, serependious identification several chimeric human parthenotes androgenotes last two decades, along with creation viable bi-maternal mice laboratory based on minor genetic interferences, raises question whether natural cases clinically healthy have gone unnoticed to science. Here we present a...
One of the most important laboratory animal species is nematode Caenorhabditis elegans, which has been used in a range research fields such as neurobiology, body development, and molecular biology. The scientific progress obtained by employing C. elegans model these areas encouraged its use new fields. potential applications concerns biological responses to hyperacceleration stress (g-force), but only few studies have evaluated response multicellular organisms extreme hypergravity conditions...
The RNA interference (RNAi) technique is a recent technology that uses double-stranded molecules to promote potent and specific gene silencing. application of this molecular biology has increased considerably, from function identification disease treatment. However, not all small interfering RNAs (siRNAs) are equally efficient, making target selection an essential procedure. Here we present Strand Analysis (SA), free online software tool able identify classify the best RNAi targets based on...
The pilocarpine-induced (PILO) model has helped elucidate the electrophysiological and molecular aspects related to mesial temporal lobe epilepsy. It been suggested that extensive cell death edema observed in brains of these animals could be induced by increased inflammatory responses, such as rapid release cytokine interleukin 1 beta (Il1b). In this study, we investigate role endogenous Il1b acute phase PILO model. Our aim is twofold. First, want determine whether it feasible silence...
During its long and rich history, medical sciences have undergone several changes of paradigms. In this quest to develop more potent drugs, less invasive surgical techniques sophisticated diagnostic approaches (among other goals) medicine has contributed decisively our well being longevity. Some these major accomplishments, which altered the course humanity are: development antibiotics, organ transplantation radiography. Medicine recently reached a new hallmark: RNA interference (RNAi),...