A5011317648- Bartonella species infections research
- Viral gastroenteritis research and epidemiology
- Animal testing and alternatives
- Animal Virus Infections Studies
- Neuroscience and Neuropharmacology Research
- Viral Infections and Vectors
- Zoonotic diseases and public health
- MicroRNA in disease regulation
- Viral Infections and Immunology Research
- Nail Diseases and Treatments
- Vector-borne infectious diseases
- Rabies epidemiology and control
- Streptococcal Infections and Treatments
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Insect and Pesticide Research
- Orthopaedic implants and arthroplasty
- Helminth infection and control
- Neurogenesis and neuroplasticity mechanisms
- Dermatologic Treatments and Research
- Trypanosoma species research and implications
- Genital Health and Disease
- Circular RNAs in diseases
- Medical Device Sterilization and Disinfection
- Vector-Borne Animal Diseases
Universidade Estadual de Campinas (UNICAMP)
2012-2022
Centro Universitário da Cidade
2018
Iowa City Public Library
2016
Seattle University
2016
Hudson Institute
2016
John Wiley & Sons (United States)
2016
Hospital de Clínicas da Unicamp
1994-2014
Bartonella species are blood-borne, re-emerging organisms, capable of causing prolonged infection with diverse disease manifestations, from asymptomatic bacteremia to chronic debilitating and death. This pathogen can survive for over a month in stored blood. However, its prevalence among blood donors is unknown, screening supplies this not routinely performed. We investigated spp. 500 Campinas, Brazil, based on cross-sectional design. Blood samples were inoculated into an enrichment liquid...
The Myrtaceae family is a common source of medicines used in the treatment numerous diseases South America. In Brazil, fruits Campomanesia species are widely to make liqueurs, juices and sweets, whereas leaves traditionally employed as medicine for dysentery, stomach problems, diarrhea, cystitis urethritis. Ethanol extracts adamantium (Myrtaceae) were evaluated against prostate cancer cells (PC-3). compound (2E)-1-(2,4-dihydroxy-6-methoxyphenyl)-3-phenylprop-2-en-1-one, cardamonin) was...
ABSTRACT Bartonella spp. are bacteria of worldwide distribution that cause asymptomatic to fatal infections in animals and humans. The most common zoonotic species is henselae , for which cats the major natural reservoir host. To better understand sp. diagnostic limitations, we determined frequency bloodstream infection 112 by comparing combining results multiple conventional nested PCRs from blood liquid culture samples. Using PCR, DNA was amplified 27.7% samples (31/112) compared 90.2%...
Murine hepatitis virus (MHV) strain 3, one of the most important inducers viral hepatitis, has been extensively studied as an organism to gain a better understanding coronavirus biology and pathogenesis. Only sequence is currently available.
Abstract Mesial temporal lobe epilepsy (MTLE) is a chronic neurological disorder characterized by the occurrence of seizures, and histopathological abnormalities in mesial structures, mainly hippocampal sclerosis (HS). We used multi‐omics approach to determine profile transcript protein expression dorsal ventral dentate gyrus (DG) Cornu Ammonis 3 (CA3) an animal model MTLE induced pilocarpine. performed label‐free proteomics RNAseq from laser‐microdissected tissue isolated...
Mesial temporal lobe epilepsy (MTLE) is a chronic neurological disorder affecting almost 40% of adult patients with epilepsy. Hippocampal sclerosis (HS) common histopathological abnormality found in MTLE. HS characterised by extensive neuronal loss different hippocampus sub-regions. In this study, we used laser microdissection-based microproteomics to determine the protein abundances regions and layers dentate gyrus (DG) an electric stimulation rodent model which displays classical damage...
Abstract We report here the first complete transcriptome analysis of dorsal (dDG) and ventral dentate gyrus (vDG) a rat epilepsy model presenting hippocampal lesion with strict resemblance to classical sclerosis (HS). collected dDG vDG by laser microdissection 15 days after electrical stimulation performed high-throughput RNA-sequencing. There were many differentially regulated genes, some which specific either two sub-regions in stimulated animals. Gene ontology indicated an enrichment...
BACKGROUND Bartonella spp. are neglected fastidious Gram‐negative bacilli. We isolated henselae from 1.2% of 500 studied blood donors and demonstrated that the bacteria remain viable in red cell units after 35 days experimental infection. Now, we aim to evaluate possibility B. transmission by transfusion a mouse model. STUDY DESIGN AND METHODS Eight BALB/c mice were intraperitoneal inoculated with 30 µL suspension 10 4 CFU/mL second group eight saline solution used as control. After 96 hours...
Abstract Objectives We compared the proteomic signatures of hippocampal lesion induced in three different animal models mesial temporal lobe epilepsy with sclerosis (MTLE+HS): systemic pilocarpine model (PILO), intracerebroventricular kainic acid (KA), and perforant pathway stimulation (PPS). Methods used shotgun proteomics to analyze proteomes find enriched biological pathways dorsal ventral dentate gyrus (DG) isolated from hippocampi models. also obtained that DG patients pharmacoresistant...
A parasitological study was undertaken to determine the health status of 15 mouse and 10 rat colonies bred in 18 Brazilian laboratory animal houses maintained under different sanitary barrier conditions which supply animals for teaching, research purposes manufacture biological products medical or veterinary use. Parasitological methods were used diagnosis mites, lices, helminthes protozoan parasites. questionnaire answered by institutions with intention obtain information about existence...
Machado-Joseph disease (MJD) or spinocerebellar ataxia type 3 (SCA3) is an autosomal dominant neurodegenerative disorder caused by expansion of the polyglutamine domain ataxin-3 (ATX3) protein. MJD/SCA3 most frequent in many countries. The mechanism underlying thought to be mainly related protein misfolding and aggregation leading neuronal dysfunction followed cell death. Currently, there are no effective treatments for patients with MJD/SCA3. Here, we report on potential use lithium...
To evaluate the effect of different energies and stacking in skin shrinkage.Three decreasing settings a fractional CO2 laser were applied to abdomen Twenty five Wistar rats divided into three groups. Group I (n=5) was histologically evaluated for microthermal zones dimensions. Groups II III (n=10 each) macroscopic with freeware ImageJ area contraction immediately after 30 60 days.No statistical significance found within zone histological dimensions (Group I) all studied. (Ablation depth:...
Murine norovirus (MNV) is a single-stranded positive-sense RNA virus of the Caliciviridae family. MNV has been reported to infect laboratory mice with ability cause lethal infections in strains lacking components innate immune response. Currently, considered most prevalent infectious agent detected mouse facilities. In this study, 22 animal facilities within Brazil were analyzed for infection. Using primers targeting conserved region viral capsid, was by RT-PCR 137 359 from all Nucleotide...
The pilocarpine-induced (PILO) model has helped elucidate the electrophysiological and molecular aspects related to mesial temporal lobe epilepsy. It been suggested that extensive cell death edema observed in brains of these animals could be induced by increased inflammatory responses, such as rapid release cytokine interleukin 1 beta (Il1b). In this study, we investigate role endogenous Il1b acute phase PILO model. Our aim is twofold. First, want determine whether it feasible silence...
Thirty-one captive maned wolves (Chrysocyon brachyurus, Illiger 1815) from 11 Zoos at the State of São Paulo, Brazil, were screened to investigate presence parasites and Salmonella infection by parasitological diagnostic methods fecal selective culture. The most frequent ecto endoparasites found Ctenocephalides felis (56.2%), Rhipicephalus sanguineus (12.5%), Ancylostoma caninum (45.1%), Strongyloides sp. (29.0%), Uncinaria stenocephala (3.2%), Capillaria Entamoeba (22.9%), Sarcocystis...
Calcium ions play an important role in several cell functions, from fertilization to death. The cytosolic Ca2+ concentration is much lower than the extracellular ([Ca2+]o). latter may markedly affect fluxes across membrane and thus cellular load. Thus, when working with preparations vitro, it keep [Ca2+]o close vivo value. In this study, we determined calcemia immature rats, for which values are currently unavailable, investigated how supraphysiological affects myocardial handling. Blood...
PURPOSE: To investigate the osseointegration properties of prototyped implants with tridimensionally interconnected pores made Ti6Al4V alloy and influence a thin calcium phosphate coating. METHODS: Bilateral critical size calvarial defects were created in thirty Wistar rats filled coated uncoated randomized fashion. The animals kept for 15, 45 90 days. Implant mechanical integration was evaluated push-out test. Bone-implant interface analyzed using scanning electron microscopy. RESULTS:...
Bartonella henselae was detected in defibrinated sheep blood employed supplementing a selective bacteria culture medium by nested PCR. We recommended that highly sensitive technical tests be run to ensure sterile for spp. isolation, since infected samples used preparation could lead false-positive results.