A5029453274- Health Systems, Economic Evaluations, Quality of Life
- Advanced MRI Techniques and Applications
- Cardiac electrophysiology and arrhythmias
- Pain Mechanisms and Treatments
- Spine and Intervertebral Disc Pathology
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Cardiovascular Function and Risk Factors
- NMR spectroscopy and applications
- Pharmaceutical Economics and Policy
- Economic and Financial Impacts of Cancer
- Cardiac Ischemia and Reperfusion
- Heart Failure Treatment and Management
- Cancer-related Molecular Pathways
- TGF-β signaling in diseases
- Chronic Myeloid Leukemia Treatments
- Diabetes Treatment and Management
- Breast Cancer Treatment Studies
- Genomics, phytochemicals, and oxidative stress
- Atomic and Subatomic Physics Research
- Ubiquitin and proteasome pathways
- Neutropenia and Cancer Infections
- Neuroscience and Neuropharmacology Research
- Mechanical and Optical Resonators
- Aldose Reductase and Taurine
Columbia University Irving Medical Center
2016-2018
University of Santa Monica
2016
Amgen (United States)
2016
National Institutes of Health
1964-2014
Columbia University
1993-2013
Emory University Hospital
1998
National Cancer Institute
1962-1965
Institute of Art
1964
National Institute of Arthritis and Musculoskeletal and Skin Diseases
1962
Bone marrow (about 0.5 ml) from au erythropoietic region is freed of blood clots by washing 1-3 min in 1 μg/ml colchicine solution (2-3 and then soaking 1-2 hr at 20-30° C a second change. For mammalian or avian marrows, the made up phosphate-buffered (pH 7) physiological NaCl solution; for amphibian, Ringer's A solution. Next specimens are soaked about 20 hypotonic as follows: mammalian, 1% Na-citrate; avian, 1:4 dilution buffered distilled water; A-distilled water, 1:1. Then they heated...
Peripheral nerve lesions provoke apoptosis in the dorsal horn of spinal cord. The cause cell death, involvement neurons, and relevance for processing somatosensory information are controversial. Here, we demonstrate a mouse model sciatic injury that glutamate-induced neurodegeneration loss γ-aminobutyric acid (GABA)ergic interneurons superficial promote transition from acute to chronic neuropathic pain. Conditional deletion Grin1, essential subunit N-methyl-d-aspartate-type glutamate...
Prostaglandin reductase 1 (PTGR1) is a highly inducible enzyme with enone activity. Previous studies demonstrated the role of rat PTGR1 in activation acylfulvene analogs, class antitumor natural product derivatives. Of these, hydroxymethylacylfulvene (HMAF) was advanced clinical development for treatment solid tumors, including prostate, ovarian, and pancreatic cancers. However, efficiency human activating acylfulvenes its potential to enhance therapeutic efficacy have remained...
Metabolic interventions that promote glucose use during ischemia have been shown to protect ischemic myocardium and improve functional recovery on reperfusion. We evaluated whether the cardioprotection afforded by high low-flow is associated with changes in sarcolemmal content of transporters, specifically GLUT-4. Isolated rat hearts were paced at 300 beats/min perfused under normal (5 mM) or (10 conditions buffer containing 0.4 mM albumin, palmitate, 70 mU/l insulin subjected 50 min 60 To...
Subcellular compartmentalization of energy stores to support different myocardial processes has been exemplified by the glycolytic control ATP-sensitive K + channel. Recent data suggest that intracellular sodium (Na i ) may also rely on glycolytically derived ATP; however, degree this dependence is unclear. To examine question, isolated, perfused rat hearts were exposed hypoxia, selectively inhibit oxidative metabolism, or iodoacetate (IAA, 100 μmol/l), glycolysis. Na and high-energy...
The feasibility of employing triple-quantum-filtered (TQF) or double-quantum-filtered (DQF) 23Na NMR spectra to monitor intracellular Na (Nain) content in isolated rat hearts perfused the absence a chemical-shift reagent (SR) was investigated. This necessitated characterization following: first, pool Nain represented by TQF (TQFin) spectrum; second, maximum extent which altered transverse relaxation times affect TQFin spectral amplitudes; and finally, situations for SR-free method can...
Reducing the risk of chronic postoperative pain through preventive analgesia is an attractive therapeutic concept. Because peripheral nerve lesions are a major cause after surgery, we tested in rats whether analgesic treatment with pregabalin (PGB) has capacity to mitigate development persistent neuropathic pain-like behavior. Starting on day spared injury or 1week later, treated continuous intrathecal infusion PGB (300 900μg/24hours) vehicle for up 28days. Rats receiving early had almost...
NRP/B, a family member of the BTB/Kelch repeat proteins, is implicated in neuronal and cancer development, as well regulation oxidative stress responses breast brain cancer. Our previous studies indicate that NRP/B-BTB/POZ domain involved dimerization NRP/B complex formation with tumor suppressor, retinoblastoma protein. Although much evidence supports potential role molecular mechanisms action on E2F transcription factors have not been elucidated.Three-dimensional modeling was used to...
Diabetes increases both the incidence of cardiovascular disease and complications myocardial infarction heart failure. Studies using diabetic animals have shown that changes in sodium transporters result alterations intracellular (Na i ) homeostasis. Because homeostasis can be due to increased entry Na + via electroneutral -K -2Cl − cotransporter (NKCC), we conducted experiments acute hearts determine if 1) net inward cation flux NKCC is increased, 2) this contributes a greater increase...
6602 Background: US health care spending continues to exceed that of other industrialized countries, in part because there is insufficient transparency as the relative value (benefit-cost) different treatment options. Comparing drug regimens based on their overall would help create a common set metrics enable better therapy decision-making and improve quality reduce costs care. Methods: We developed methodology review, synthesize assess evidence drug's performance across three major domains...
The primary aim of this study is to model the cost per 1md/dL reduction in LDL-C with evolocumab 140mg and alirocumab 75/150mg a large national health plan. Patients clinical atherosclerotic cardiovascular disease (ASCVD) >100 mg/dl (mean = 125.2 mg/dl; SD 25.1 mg/dl) were obtained from Optum Research Database Jan 1, 2012 2014. An economic using Monte Carlo simulations was developed validated estimate 1mg/dL LDL-C. Inputs include efficacy based on published Navarese meta-analysis (evolocumab...
Abstract Smads are intracellular signaling transducers of TGF-β family ligands. Through activation, phosphorylated R-Smads (Smad2/3) form active heteromeric complexes with co-Smad (Smad4) and translocate to the nucleus where they interact chromatin assemble transcriptional machinery various target genes. Evidence also indicates that involved in modulation via interactions histone modifiers. The role pre-existing nuclear modulation, however, has not been described, nor have molecular...