A5016904027- Adenosine and Purinergic Signaling
- Peptidase Inhibition and Analysis
- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- Glioma Diagnosis and Treatment
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Nanoparticle-Based Drug Delivery
- Nanoplatforms for cancer theranostics
- Neuroinflammation and Neurodegeneration Mechanisms
- Radiopharmaceutical Chemistry and Applications
- Brain Metastases and Treatment
- Neurological Complications and Syndromes
- Bipolar Disorder and Treatment
- Neurological Disorders and Treatments
- Curcumin's Biomedical Applications
- Chromatography in Natural Products
- Periodontal Regeneration and Treatments
- Long-Term Effects of COVID-19
- Neuroscience of respiration and sleep
- Neuroendocrine regulation and behavior
- Macrophage Migration Inhibitory Factor
- Metabolism and Genetic Disorders
- Hedgehog Signaling Pathway Studies
- Ferroptosis and cancer prognosis
Universidade Federal do Rio Grande do Sul
2018-2024
Pontifícia Universidade Católica do Rio Grande do Sul
2024
Hospital de Clínicas de Porto Alegre
2017
Universidade Federal de Ciências da Saúde de Porto Alegre
2017
Ectonucleotidases modulate inflammatory responses by balancing extracellular ATP and adenosine (ADO) might be involved in COVID-19 immunopathogenesis. Here, we explored the contribution of nucleotide metabolism to severity mild severe cases disease. We verified that gene expression ectonucleotidases is reduced whole blood patients with negatively correlated levels CRP, an marker disease severity. In line these findings, present higher plasma ADO when compared healthy controls. Cell...
Adipose-derived stem cells (ADSC) are multipotent implicated in tissue homeostasis. Obesity represents a chronic inflammatory disease associated with metabolic dysfunction and age-related mechanisms, progressive accumulation of senescent compromised ADSC function. In this study, we aimed to explore mechanisms the environment present obesity modulating phenotype. We evaluated phenotypic functional alterations through 18 days treatment. were cultivated conditioned medium supplemented pool...
Human glioblastoma cells are strikingly refractory to ATP-stimulated, P2X7 receptor (P2X7R)-mediated cytotoxicity. To elucidate the mechanistic basis of this feature, we investigated P2X7R-dependent responses in wild type and P2X7R-transfected U138 cells. Mouse GL261 glioma were used as an additional control. Here, report that expressed P2X7R very low level. Contrary human cells, mouse showed strong expression responses. Transfection P2RX7 into fully restored transfection conferred a...
Aim: To characterize a method to isolate glioma-derived extracellular vesicles (GEVs) and understand their role in immune system modulation glioma progression. Materials & methods: GEVs were isolated by differential centrifugation from C6 cell supernatant characterized size expression of CD9, HSP70, CD39 CD73. The model was performed injecting cells into the right striatum Wistar rats following groups: controls (C6 alone), coinjection + GEVs) intranasal administration followed cells, tumor...
Prostate cancer (PC) is the most diagnosed tumor in males and ranks as second leading cause of male mortality western world. The CD39 CD73 enzymes play a crucial role regulation by degrading nucleotides forming nucleosides. This study aimed to investigate expression potential therapeutic targets for PC. initial part this retrospectively analyzed tissue samples from 23 PC patients. Using TissueFAXSTM cytometry platform, we found significantly higher levels CD39-labeling its intensity compared...
Aim: To evaluate the antitumor efficacy of bevacizumab-functionalized nanocapsules in a rat glioblastoma model after pretreatment with functionalized peptide-specific to epidermal growth factor receptor variant III. Materials & methods: Nanocapsules were prepared, physicochemical characterized and intranasally administered rats. Parameters such as tumor size, histopathological characteristics infiltration CD8+ T lymphocytes evaluated. Results: The strategy treatment resulted reduction 87%...
What is the central question of this study? How does moderate-intensity aerobic exercise affect behaviour purinergic enzymes in sedentary, overweight and physically active subjects? relationship between inflammatory responses triggered by exercise? main finding its importance? Moderate-intensity modifies activity levels nucleotides nucleosides. These results are similar subjects with different biological characteristics. 5'-Nucleotidase adenosine associated responses. This study suggests...
Tissue accumulation and high urinary excretion of ethylmalonic acid (EMA) are found in encephalopathy (EE), an inherited disorder associated with cerebral cerebellar atrophy whose pathogenesis is poorly established. The vitro vivo effects EMA on bioenergetics redox homeostasis were investigated rat cerebellum. For the studies, cerebellum preparations exposed to EMA, whereas intracerebellar injection was used for evaluation. reduced state 3 uncoupled respiration succinate-, glutamate-,...
The conversion of adenosine to inosine is catalyzed by deaminase (ADA) (EC 3.5.4.4), which has two isoforms in humans (ADA1 and ADA2) belongs the zinc-dependent hydrolase family. ADA modulates lymphocyte function differentiation, regulates inflammatory immune responses. This study investigated activity lymphocyte-rich peripheral blood mononuclear cells (PBMCs) absence disease. viability PBMCs isolated from kept 0.9% saline solution at 4-8°C was analyzed over 20 h. incubation time biochemical...
Glioblastoma (GB) is a histological and genetically heterogeneous brain tumor that highly proliferative vascularized. The prognosis poor with currently available treatment. In this study, we evaluated the cytotoxicity antiangiogenic activity of doxorubicin-loaded-chitosan-coated-arginylglycylaspartic acid-functionalized-poly(ε-caprolactone)-alpha bisabolol-LNC (AB-DOX-LNC-L-C-RGD). nanoformulation was prepared by self-assembling followed interfacial reactions, physicochemically characterized...
Abstract Microglia, the resident immune cells of Central Nervous System (CNS), play crucial roles in homeostasis, responses, and modulation neuronal functions. In brain tumors, microglia are difficult to distinguish from infiltrating macrophages collectively called Tumor-Associated Macrophages (TAMs). While these exhibit plasticity, support tumor growth, suppress antitumor their identity function remain largely unknown. Cellular senescence, a state growth arrest associated with upregulation...