A5075178865- Adenosine and Purinergic Signaling
- Pancreatic function and diabetes
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Bipolar Disorder and Treatment
- Liver Disease Diagnosis and Treatment
- Autism Spectrum Disorder Research
- Nitric Oxide and Endothelin Effects
- Neuroscience and Neural Engineering
- Diet, Metabolism, and Disease
- Receptor Mechanisms and Signaling
- Neurological disorders and treatments
- Ion channel regulation and function
- Adolescent and Pediatric Healthcare
- Vagus Nerve Stimulation Research
- Cell Adhesion Molecules Research
- Restless Legs Syndrome Research
- Electromagnetic Fields and Biological Effects
- Angiogenesis and VEGF in Cancer
- Neuroscience and Neuropharmacology Research
- Genetic Neurodegenerative Diseases
- Nuclear Receptors and Signaling
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
Rutgers, The State University of New Jersey
2020-2024
Universidade de São Paulo
2016-2022
Bone marrow metastasis occurs in approximately 350,000 patients that annually die the U.S. alone. In view of importance tumor cell migration into bone marrow, we have here investigated effects various concentrations stromal cell-derived factor-1 (SDF-1), bradykinin- and ATP on metastasis. We show for first time bradykinin augmented chemotactic responsiveness neuroblastoma cells to SDF-1 concentrations, encountered under physiological conditions. Bradykinin upregulated VEGF expression,...
Parkinson's disease (PD) is a neurodegenerative disorder characterized by decreased dopamine bioavailability in the substantia nigra and striatum. Taking into account that adenosine-5'-triphosphate (ATP) its metabolites are intensely released 6-hydroxydopamine (6-OHDA) animal model of PD, screening purinergic receptor gene expression was performed. Effects pharmacological P2Y6 or P2X7 antagonism were studied preventing reversing hemiparkinsonian behavior dopaminergic deficits this model....
Excess consumption of carbohydrates, fat and calories leads to non-alcoholic fatty liver disease (NAFLD) hepatic insulin resistance; these are major factors in the pathogenesis type II diabetes. Hormones catecholamines acting through G-protein coupled receptors (GPCRs) linked phospholipase C (PLC) increases cytosolic Ca2+ ([Ca2+ ]c ) regulate many metabolic functions liver. In intact liver, catabolic hormones such as glucagon, vasopressin integrate synergize frequency extent which [Ca2+...
Abstract Objective The endothelium regulates crucial aspects of vascular function, including hemostasis, vasomotor tone, proliferation, immune cell adhesion, and microvascular permeability. Endothelial cells (ECs), especially in arterioles, are pivotal for flow distribution peripheral resistance regulation. Investigating physiology, particularly ECs, demands precise isolation culturing techniques. Methods Freshly isolated ECs vital examining protein expression, ion channel behavior, calcium...
Abstract Oligogenic inheritance of autism spectrum disorder (ASD) has been supported by several studies. However, little is known about how the risk variants interact and converge on causative neurobiological pathways. We identified in an ASD proband deleterious compound heterozygous missense Reelin ( RELN ) gene, a de novo splicing variant Cav3.2 calcium channel CACNA1H gene. Here, using iPSC-derived neural progenitor cells (NPCs) heterologous expression system, we show that leads to...
Extracellular agonists linked to inositol-1,4,5-trisphosphate (IP3) formation elicit cytosolic Ca2+ oscillations in many cell types, but despite a common signaling pathway, distinct agonist-specific spike patterns are observed. Using qPCR, we show that rat hepatocytes express multiple purinergic P2Y and P2X receptors (R). ADP acting through P2Y1R elicits narrow oscillations, whereas UTP P2Y2R broad with composite observed for ATP. P2XRs do not play role at physiological agonist levels. The...
Abstract Extracellular nucleotides are key regulators of liver physiology. In primary rat hepatocytes, P2Y1 receptor (P2Y1R) activation by ADP generates cytosolic calcium ([Ca 2+ ] c ) oscillations with narrow spikes, whereas P2Y2/4R UTP led to more complex broad [Ca oscillations. Both oscillation signatures were observed the common agonist ATP. Inhibition Gα q signaling YM-254890 abolished ATP-induced oscillations, indicating that they depend on inositol 1,4,5-trisphosphate (IP 3 ), and not...
Abstract Background Fragile X syndrome, the major cause of inherited intellectual disability among men, is due to deficiency synaptic functional regulator FMR1 protein (FMRP), encoded by FMRP translational 1 ( ) gene. alternative splicing produces distinct transcripts that may consequently impact roles. In without exon 14 reading frame shifted. For deepening current knowledge differential expression Fmr1 along rat nervous system development, we conducted a descriptive study employing...
Abstract Excess consumption of carbohydrates, fat, and calories leads to non-alcoholic fatty liver disease (NAFLD) hepatic insulin resistance; major factors in the pathogenesis type II diabetes. Hormones catecholamines acting through G-protein coupled receptors (GPCRs) linked phospholipase C (PLC) increases cytosolic Ca 2+ ([Ca ] c ) regulate many metabolic functions liver. In intact liver, catabolic hormones such as glucagon, vasopressin integrate synergize frequency extent which [Ca waves...