A5011660309- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Bioinformatics and Genomic Networks
- Gene expression and cancer classification
- Blood Coagulation and Thrombosis Mechanisms
- Genomics and Chromatin Dynamics
- Bone and Dental Protein Studies
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- CRISPR and Genetic Engineering
- Mechanisms of cancer metastasis
- Gene Regulatory Network Analysis
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Viral Infectious Diseases and Gene Expression in Insects
- Hemophilia Treatment and Research
- Platelet Disorders and Treatments
- Autophagy in Disease and Therapy
- Estrogen and related hormone effects
- Cancer Research and Treatments
- TGF-β signaling in diseases
- Adenosine and Purinergic Signaling
- DNA Repair Mechanisms
- Silk-based biomaterials and applications
- Anodic Oxide Films and Nanostructures
Universidade de São Paulo
2009-2023
Instituto Butantan
2010
University of Illinois Chicago
2001
The proteasome is the primary contributor in intracellular proteolysis. Oxidized or unstructured proteins can be degraded via a ubiquitin- and ATP-independent process by free 20S (20SPT). mechanism which these enter catalytic chamber not understood thus far, although 20SPT gating conformation considered to an important barrier allowing entrance. We have previously shown that S-glutathiolation of post-translational modification affecting proteasomal activities.The goal this work was...
DNA microarrays have become a powerful tool to describe gene expression profiles associated with different cellular states, various phenotypes and responses drugs other extra- or intra-cellular perturbations. In order cluster co-expressed genes and/or construct regulatory networks, definition of distance similarity between measured data is usually required, the most common choices being Pearson's Spearman's correlations. Here, we evaluate these two methods also compare them third one, namely...
Hypoxia and necrosis are fundamental features of glioma, their emergence is critical for the rapid biological progression this fatal tumor. The presence vaso-occlusive thrombus higher in grade IV tumors [glioblastoma multiforme (GBM)] compared with lower tumors, suggesting that procoagulant properties tumor contribute to its aggressive behavior, as well establishment hypoxia necrosis. Tissue factor (TF), primary cellular initiator coagulation, overexpressed GBMs likely favors a thrombotic...
Abstract Embryonic carcinoma cells are widely used models for studying the mechanisms of proliferation and differentiation occurring during early embryogenesis. We have now investigated how down‐regulation P2X2 P2X7 receptor expression by RNA interference (RNAi) affects neural phenotype specification P19 embryonal cells. Wild‐type or stably expressing shRNAs targeting were induced to differentiate into neurons glial in presence retinoic acid. Silencing along promoted cell an increase...
Glioblastoma multiforme is the most common and lethal of central nervous system glial-derived tumors. RECK suppresses tumor invasion by negatively regulating at least three members matrix metalloproteinase family: MMP-9, MMP-2, MT1-MMP. A positive correlation has been observed between abundance expression in samples a more favorable prognosis for patients with several types In present study, novel alternatively spliced variants gene: RECK-B RECK-I were isolated RT-PCR sequenced. The levels...
Summary The tail bleeding model using haemophilic mice has been used as one of the standard assays for efficacy evaluation novel antihaemophilic therapies at preclinical level. A number different configurations and endpoints have proposed in literature this model, hindering interlaboratory comparisons. particular configuration, known survival assay (TBS), adopted by several groups, involves measuring ability conscious to survive exsanguination following transection. Major limitations...
The CHD7 (Chromodomain Helicase DNA binding protein 7) gene encodes a member of the chromodomain family ATP-dependent chromatin remodeling enzymes. Mutations in are found individuals with CHARGE, syndrome characterized by multiple birth malformations several tissues. was identified as partner PBAF complex (Polybromo and BRG Associated Factor containing complex) playing central role transcriptional reprogramming process associated to formation multipotent migratory neural crest, transient...
Upon searching for glucocorticoid-regulated cDNA sequences associated with the transformed to normal phenotypic reversion of C6/ST1 rat glioma cells, we identified Nrp/b (nuclear restrict protein in brain) as a novel gene. Here report on identification and functional characterization complete sequence encoding NRP/B protein. The cloned presented 1767 nucleotides open-reading frame 589 amino acids residues containing BTB/POZ (broad complex Tramtrack bric-a-brac/Pox virus zinc finger) domain...
During the tumorigenic process, cancer cells may become overly dependent on activity of backup cellular pathways for their survival, representing vulnerabilities that could be exploited as therapeutic targets. Certain molecular manifest a synthetic lethality relationship, and identification characterization new lethal interactions pave way development approaches human cancer. Our goal was to investigate possible interaction between member Chromodomain Helicase DNA binding proteins family...
The type I herpes simplex virus VP22 tegument protein is abundant and well known for its ability to translocate proteins from one cell the other. In spite of some reports questioning by attributing results observed fixation artifacts or simple attachment membrane, has been used deliver several into different types, triggering expected response. However, question enter stem cells not addressed. We investigated whether could be as a tool applied in research differentiation due capacity...
Several gene regulatory network models containing concepts of directionality at the edges have been proposed. However, only a few reports an interpretable definition directionality. Here, differently from standard causality concept defined by Pearl, we introduce contagion in order to infer edges, i.e., asymmetries expression dependences networks. Moreover, present bootstrap algorithm test concept. This technique was applied simulated data and, also, actual large sample biological data....
Abstract REversion-inducing Cysteine-rich protein with Kazal motifs (RECK) encodes a membrane-anchored which suppresses both invasion and metastasis by negatively regulating at least three MMP′s, namely: MMP-9, MMP-2 MT1-MMP. Matrix metalloproteinase (MMP) family members belong to the group of Zn2+ dependent proteolytic enzymes, are key elements responsible for extracellular matrix (MEC) collapse, being, therefore, directly involved in tumor invasiveness metastasis. MEC degradation MMPs not...