A5028752327- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Neuroscience and Neural Engineering
- Ion Channels and Receptors
- Retinal Development and Disorders
- Photoreceptor and optogenetics research
- Parathyroid Disorders and Treatments
- Cardiac electrophysiology and arrhythmias
- Magnesium in Health and Disease
- Amino Acid Enzymes and Metabolism
- Neuroscience and Neuropharmacology Research
- Erythrocyte Function and Pathophysiology
- CCD and CMOS Imaging Sensors
- Cytokine Signaling Pathways and Interactions
- Genetic Syndromes and Imprinting
- RNA Research and Splicing
- Phytochemicals and Antioxidant Activities
- EEG and Brain-Computer Interfaces
- Receptor Mechanisms and Signaling
- Plant Stress Responses and Tolerance
- Aldose Reductase and Taurine
- Endometriosis Research and Treatment
- Reproductive System and Pregnancy
- Metabolism, Diabetes, and Cancer
- Electrolyte and hormonal disorders
Radboud University Medical Center
2023-2025
Radboud University Nijmegen
2023-2025
University Medical Center
2024
University of Freiburg
2024
Henry Ford Health System
2024
Bernstein Center for Computational Neuroscience Freiburg
2024
Institute of Ophthalmology
2024
University Hospital Leipzig
2023
Leipzig University
2019-2023
Academic Center for Education, Culture and Research
2021
<b><i>Background/Aims: </i></b>Klotho, a protein mainly produced in the kidney and released into circulating blood, contributes to negative regulation of 1,25(OH)<sub>2</sub>D<sub>3</sub> formation is thus powerful regulator mineral metabolism. As β-glucuronidase, alpha Klotho further regulates stability several carriers channels plasma membrane channel transporter activity. Accordingly, participates diverse functions seemingly unrelated...
Amyotrophic lateral sclerosis (ALS) is a lethal disease characterized by motor neuron degeneration and associated with aggregation of nuclear RNA-binding proteins (RBPs), including FUS. How FUS neurodegeneration are prevented in healthy neurons remain critically unanswered questions. Here, we use combination ALS patient autopsy tissue induced pluripotent stem cell-derived to study the effects mutations on RBP homeostasis. We show that FUS' tendency aggregate normally buffered interacting...
Background/Aims: Janus-activated kinase-2 JAK2 participates in the signaling of several hormones including growth hormone, fosters tumor and modifies activity Na+ coupled nutrient transporters. Peptide uptake into intestinal cells is accomplished by electrogenic peptide transporters PEPT1 PEPT2. The present study thus explored whether contributes to regulation PEPT2 activity. Methods: cRNA encoding either or was injected Xenopus oocytes with without additional injection wild type JAK2,...
Objective: Visual adaptation is a physiological and perceptual process by which the visual system adjusts to changes in environment or stimuli. This fundamental how we perceive world around us allows our efficiently encode information. The retina incorporates adaptaion with its dozens of functionally distinct retinal ganglion cell types. Meanwhile, field prostheses increasing understanding electrical cell-specific stimulation. However, very little known about interaction stimulation on...
Abstract The rd2 mouse model, characterized by a mutation in the Prph2 gene, exhibits abnormal development of photoreceptor outer segments, resulting progressive retinal degeneration. While correlation between poly-ADP-ribose polymerase (PARP) activity and degeneration rod photoreceptors is established specific mechanism driving cone this model remains unclear. Furthermore, it yet to be determined whether inhibiting PARP can effectively impede context. We demonstrated that inhibitors...
Background/Aims: The serine/threonine kinase Tau-tubulin-kinase 2 (TTBK2) is expressed in various tissues including kidney, liver and brain. Loss of function mutations TTBK2 lead to autosomal dominant spinocerebellar ataxia type 11 (SCA11). Cell survival fostered by cellular accumulation organic osmolytes. Carriers accomplishing osmolytes include the Na+, Cl--coupled betaine/γ-amino-butyric acid transporter BGT1. present study explored whether participates regulation BGT1 activity. Methods:...
Abstract In mature erythrocytes, glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate (6PGDH) yield NADPH, a crucial cofactor of the enzyme glutathione reductase (GR) converting disulfide (GSSG) into its reduced state (GSH). GSH is essential for detoxification processes in survival erythrocytes. We explored whether anti-inflammatory compounds Bay 11–7082, parthenolide dimethyl fumarate (DMF) were able to completely deplete common target (GSH), impair function upstream enzymes...
Background: Serum &amp; Glucocorticoid Regulated Kinase 1 (SGK1) plays a fundamental role in ion and solute transport processes epithelia. In the endometrium, down-regulation of SGK1 during window receptivity facilitates embryo implantation whereas expression constitutively active mutant murine uterus blocks implantation. Methods/Results: Here, we report that treatment endometrial epithelial cells with specific inhibitors phosphoinositide 3-kinase (PI3K)/AKT activity pathway results...
Neuromuscular circuits (NMCs) are vital for voluntary movement, and effective models of NMCs needed to understand the pathogenesis of, as well identify treatments for, multiple diseases, including Duchenne's muscular dystrophy amyotrophic lateral sclerosis. Microfluidics ideal recapitulating central peripheral compartments NMCs, but myotubes often detach before functional formed. In addition, microfluidic systems limited a single experimental unit, which significantly limits their...
The Janus-activated kinase-2 JAK2 is involved in the signaling of leptin and erythropoietin receptors mediates neuroprotective effects hormones. In theory, could be effective through modulation glutamate transporters, carriers accounting for clearance released during neurotransmission. present study thus elucidated effect on transporters EAAT1, EAAT2, EAAT3 EAAT4. To this end, cRNA encoding was injected into Xenopus oocytes with or without transport estimated from induced current (Iglu)....
JAK2 (Janus kinase-2) is activated by cell shrinkage and may thus participate in volume regulation. Cell regulatory ion channels include the small conductance Cl(-) ClC-2. The present study explored whether influences ClC-2 activity. To this end, was expressed Xenopus oocytes with or without wild type JAK2, active (V617F)JAK2 inactive (K882E)JAK2 channel activity determined dual electrode voltage clamp. Expression of followed a marked increase membrane conductance. significantly decreased...
<b><i>Background/Aims: </i></b>The Na<sup>+</sup>-coupled phosphate transporter NaPi-IIa is the main carrier accomplishing renal tubular reabsorption. It driven by electrochemical Na<sup>+</sup> gradient across apical cell membrane, which maintained extrusion basolateral membrane through Na<sup>+</sup>/K<sup>+</sup> ATPase. The operation of thus requires energy in order to avoid cellular accumulation and...
<b><i>Background/Aims: </i></b>SPAK (SPS1-related proline/alanine-rich kinase) and OSR1 (oxidative stress-responsive kinase 1) are powerful regulators of diverse transport processes. Both kinases activated by cell shrinkage participate in stimulation regulatory volume increase (RVI). Execution RVI involves inhibition Cl<sup>-</sup> channels. The present study explored whether SPAK and/or regulate the activity channel ClC-2. <b><i>Methods:...
SPAK (STE20-related proline/alanine-rich kinase) is a powerful regulator of renal tubular ion transport and blood pressure. Moreover, contributes to the regulation cell volume. Little known, however, about role in or organic solutes. The present study thus addressed influence on peptide transporters PEPT1 PEPT2.To this end, cRNA encoding PEPT2 were injected into Xenopus laevis oocytes without with additional injection wild-type, SPAK, WNK1 insensitive inactive (T233A)SPAK, constitutively...
<b><i>Background/Aims: </i></b>OSR1 (oxidative-stress-responsive kinase 1) participates in the regulation of renal tubular ion transport, cell volume and blood pressure. Whether OSR1 contributes to organic solute transport remained; however, elusive. The present study thus explored sensitivity peptide transporters PEPT1 PEPT2. <b><i>Methods: </i></b>cRNA encoding or PEPT2 were injected into <i>Xenopus</i> oocytes without with...
Janus kinase 2 (JAK2) contributes to intracellular signaling of leptin and erythropoietin, hormones protecting cells during energy depletion. The present study explores whether JAK2 is activated by depletion regulates Na + /K -ATPase, the major energy-consuming pump. In Jurkat cells, activity was determined radioactive assay, phosphorylated detected Western blotting, ATP levels measured luciferase as well -ATPase α1-subunit transcript protein abundance real-time PCR respectively....
Background/Aims: Janus kinase-3 (JAK3) is activated during energy depletion. Energy-consuming pumps include the Na+/K+-ATPase. The present study explored whether JAK3 regulates Na+/K+-ATPase in dendritic cells (DCs). Methods: Ouabain (100 µM)-sensitive (Iouabain) and K+-induced (Ipump) outward currents were determined by utilizing whole cell patch-clamp, α1-subunit mRNA levels RT-PCR, protein abundance flow cytometry or immunofluorescence, cellular ATP luciferase-assay DCs from bone marrow...
Background/Aims: T-lymphocyte activation and function critically depends on Ca2+ signaling, which is regulated by store operated entry (SOCE). Human mouse T lymphocytes express AMP activated kinase AMPKα1, rapidly following elevation of cytosolic concentration ([Ca2+]i) treatment the cells with ionophore or inhibition endosomal ATPase thapsigargin. AMPK further triggering cell antigen receptor (TCR). The present study explored whether influences Ca2+-sensitive regulation function. Methods:...