The fibroblast growth factor (FGF23) plasma level is high in cardiac and renal failure associated with poor clinical prognosis of these disorders. Both diseases are paralleled by hyperaldosteronism. Excessive FGF23 levels hyperaldosteronism further observed Klotho-deficient mice. present study explored a putative aldosterone sensitivity Fgf23 transcription secretion the involvement sensitive serum & glucocorticoid inducible kinase SGK1, SGK1 NFκB store operated Ca2+ entry (SOCE). Serum were...

Objectives: Diabetic nephropathy (DN) is the most common microvascular complication of diabetes mellitus. This study investigated mechanism triptolide (TP) in podocyte injury DN.Methods: DN mouse models were established by feeding with a high-fat diet and injecting streptozocin MPC5 induced high-glucose (HG), followed TP treatment. Fasting blood glucose renal function indicators, such as 24 h urine albumin (UAlb), serum creatinine (SCr), urea nitrogen (BUN), kidney/body weight ratio mice...

Abstract Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst ), is an air-borne fungal disease, and its spores can be spread far away from origin to colonize in new territory, causing inter-regional epidemics. Xinjiang not only important independent epidemic region other rust epidemiological regions China but also has distinguished genetic structure spatial features. However, the inoculum source of remained unknown. It clear whether inocula Central Asian countries migrate...

Both mechanical loading and intracellular autophagy play important roles in bone homeostasis; however, their relationship remains largely unexplored. The objectives of this study were to determine whether osteocytes undergo upon fluid shear stress (FSS) the correlation between mechanically induced ATP metabolism. Autophagic vacuoles observed by transmission electron microscopy (TEM) osteocyte-like MLO-Y4 cells subjected FSS. Increased autophagic flux was further confirmed increased amount...

Amino acids are an essential group of compounds involved in protein synthesis and various metabolic immune reactions the human body. Chinese jujubes (Ziziphus jujuba Mill.) important fruit medicinal plant which native to China have been introduced into around 50 countries. However, systematic research on composition content diversity amino jujube is still lacking. In this experiment, acid contents dominant 161 cultivars were determined by HPLC. Of twenty-one kinds detected, a total fourteen...

In recent years, phase change materials (PCMs) have been widely used in waste heat utilization, buildings, and solar wind energy, but with a huge limitation from the low thermal conductivity, photothermal conversion efficiency, latent heat. Organic PCMs are eyecatching because of its high storage capability reliability, they still suffer lack sharp stability. Here, we prepared sharp-stable by establishing carbon material frame system consisting graphene oxide (GO) biochar. particular,...

Driver anger has become a severe transportation problem resulting in significant injuries and fatalities. The rapid development of intelligent systems provided new opportunities for dynamic angry driving regulations. However, the regulation qualities different visual attributes under parameters are not yet clear. In this article, we investigate quality during (color: cold/warm; symbol: flat/simulated; expression: positive/negative). Twenty-one drivers drove nine times ( <italic...

Significance The PI3 kinase-dependent inactivation of glycogen synthase kinase (GSK) 3 is an important aspect normal insulin signaling. Surprisingly, transgenic mice expressing kinase-resistant GSK3 (gsk3 KI ) have been found not to be insulin-resistant. We show that gsk3 are even protected from the development metabolic syndrome characterized by resistance, obesity, and dyslipidemia, which all could readily induced a high-fat diet in WT mice. affects millions patients associated with high...

Non-esterified fatty acids (NEFAs) promote de novo lipogenesis, which resulted in abnormal hepatic lipid accumulation, by NFκB-Orai1 pathway. Oxidative stress and endoplasmic reticulum (ER) have been recognized as key mechanisms non-alcoholic liver disease (NAFLD) pathogenesis. Whether Orai1 facilitates oxidative remains unknown. The rat model of NAFLD was constructed feeding high-fat diet (HFD). BRL-3A cells were treated NEFAs, Orai1inhibtor BTP2, NFκB inhibitor wogonin or small interfering...

Arctigenin is a phenylpropanoid dibenzylbutyrolactone lignan compound possessing antitumor, anti-inflammatory, anti-influenza, antioxidant, antibacterial, and hypoglycaemic activities. Our previous study demonstrated that arctigenin exerts neuroprotective effects both <i>in vitro</i> vivo</i> in Parkinson's disease model. However, the exact mechanism through which improves amyloid beta-induced memory impairment by inhibiting production of hyperphosphorylated tau protein unknown. Amyloid...

Chitosan (CTS) is a deacetylated derivative of chitin that involved in adaptive response to abiotic stresses. However, the regulatory role CTS heat tolerance still not fully understood plants, especially grass species. The aim this study was investigate whether could reduce heat-induced senescence and damage creeping bentgrass associated with alterations antioxidant defense, chlorophyll (Chl) metabolism, shock pathway. Plants were pretreated exogenously or without (0.1 g L

Transforming growth factor-β (TGF-β) is a cytokine produced by many cell types and implicated in growth, differentiation, apoptosis, inflammation. It stimulates store-operated calcium entry (SOCE) through the release-activated (CRAC) channel Orai1/Stim1 endometrial Ishikawa cells. Bone cells generate fibroblast factor (FGF) 23, which inhibits renal phosphate reabsorption 1,25(OH)2D3 formation concert with its co-receptor Klotho. Moreover, Klotho FGF23 counteract aging age-related clinical...

Hypocalcemia in dairy cows is often associated with inflammation-related disorders such as metritis and mastitis. The protein encoded by the Ca2+ release-activated calcium modulator 1 (ORAI1) gene a membrane channel subunit that activated when stores are depleted. Polymorphonuclear neutrophils (PMNL) have crucial role defense against infection through migration, adhesion, chemotaxis, phagocytosis, reactive oxygen species (ROS) production response to pathogens. Whether hypocalcemia affects...

The store-operated Ca2+ entry (SOCE) moiety ORAI calcium release-activated modulator 1 (ORAI1) located in the endoplasmic reticulum (ER) participates key cellular functions such as protein folding, transport, and secretion, lipid metabolism. We used an vitro approach to test whether exogenous fatty acids alter ORAI1 signaling explore potential consequences on mitochondrial dysfunction ER stress. First, hepatocytes isolated from 4 healthy female calves (1 d old, 40–50 kg) were challenged with...

Fatty acid accumulation in hepatocytes induced by high concentrations of fatty acids due to lipolysis and the associated oxidative damage they cause occur most frequently after calving. Because their role esterification acids, diacylglycerol acyltransferase isoforms (DGAT1 DGAT2) could play a susceptibility dairy cows develop liver. To gain mechanistic insights, we performed vivo vitro analyses using liver biopsies or isolated primary hepatocytes. The study (n = 5 cows/group) involved...

High concentrations of free fatty acids (FFAs) caused by negative energy balance render the cow more prone to inflammatory diseases in part due an imbalance types immune cells and their specific functions. We previously demonstrated that ORAI calcium release-activated modulator 1 (ORAI1) was associated with increased CD4+ Th17 content, but precise mechanisms remain unclear. The purpose this study evaluate efficacy FFAs on T cell response. dairy cows transcript level pro-inflammatory factor...

With the wide access to data and advanced technologies, organizations firms prefer use data-based interpretable analytics deal with uncertain cognitive decision-making problems. In this regard, study considers quantitative qualitative variables, propose a multi-dimensional decision framework based on nested probabilistic linguistic term sets. Under framework, XGBoost algorithm, one of machine learning methods, is conducted capture importance attributes by using historical data, further...

A unique measure of inflammatory evaluation, the systemic inflammation response index (SIRI) may offer useful data for diagnosis and risk assessment a number diseases. The aim this study was to investigate relationship between SIRI femur bone mineral density (BMD) in US adults. association BMD examined using multivariate logistic regression, sensitivity analysis, smoothing curve fitting from National Health Nutrition Examination Survey (NHANES) 2005–2010, 2013–2014, 2017–2018. Subgroup...