A5057583138- Cellular Mechanics and Interactions
- Force Microscopy Techniques and Applications
- Cell Adhesion Molecules Research
- Collagen: Extraction and Characterization
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Corneal Surgery and Treatments
- Skin and Cellular Biology Research
- Polymer Surface Interaction Studies
- Glycosylation and Glycoproteins Research
- Silk-based biomaterials and applications
- Advanced Electron Microscopy Techniques and Applications
- Proteoglycans and glycosaminoglycans research
- Protein Kinase Regulation and GTPase Signaling
- S100 Proteins and Annexins
- Integrated Circuits and Semiconductor Failure Analysis
- Colorectal Cancer Surgical Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Corneal surgery and disorders
- Civil and Structural Engineering Research
- Colorectal Cancer Screening and Detection
- Metal and Thin Film Mechanics
- Protease and Inhibitor Mechanisms
- Nanofabrication and Lithography Techniques
Life Science Institute
2020-2024
Kanazawa University
2020-2024
University Hospital Heidelberg
2014-2023
Heidelberg University
2010-2023
Karlsruhe Institute of Technology
2011-2021
Karlsruhe University of Education
2015
In-Q-Tel
2015
TU Dresden
2005-2012
Ludwig-Maximilians-Universität München
2011
National Center for Genetic Engineering and Biotechnology
2006-2007
Two-component 3D polymer scaffolds with distinct mechanical and protein-binding properties are fabricated using direct laser writing. With this method, full control over the formation of cell adhesion sites and, consequently, shape in three dimensions can be achieved. Detailed facts importance to specialist readers published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted by authors. Please note: The publisher is...
We have characterized early steps of α 2 β 1 integrin-mediated cell adhesion to a collagen type I matrix by using single-cell force spectroscopy. In agreement with the role as receptor, -expressing Chinese hamster ovary (CHO)-A2 cells spread rapidly on matrix, whereas -negative CHO wild-type adhered poorly. Probing CHO-A2 detachment forces over contact time range 600 s revealed nonlinear response. During first 60 s, increased slowly, and associated smallest rupture events were consistent...
Elastic 3D scaffolds (see figure) are fabricated into a biocompatible photoresist using direct laser writing. These can be rhythmically deformed by single beating cardiomyocytes and calibration with atomic force microscopy indicates that cellular forces down to 10–20 nN detectable the setup. Detailed facts of importance specialist readers published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted authors. Please note:...
Matrixmetalloproteinases (MMPs) comprise a family of zinc-dependent endopeptidases which are involved in angiogenesis, tumor invasion and metastatic formation. Up to date, the prognostic relevance MMPs serum patients with colon cancer remains unknown. Thus, we wanted assess an expression pattern homogenous cohort their potential as biomarkers. Differences MMP7, MMP10 MMP12 78 specimens adenocarcinoma healthy control group were assessed using Luminex-100 technologies. Subsequently, correlated...
Atomic force microscopy (AFM) can visualize the dynamics of single biomolecules under near-physiological conditions. However, scanning tip probes only molecular surface with limited resolution, missing details required to fully deduce functional mechanisms from imaging alone. To overcome such drawbacks, we developed a computational framework reconstruct 3D atomistic structures AFM scans, employing simulation and automatized fitting experimental images. We provide applications images ranging...
Atomic force microscopy (AFM) can produce high-resolution topographic images of biological samples in physiologically relevant environments and is therefore well suited for the imaging cellular surfaces. In this work we have investigated focal adhesion complexes by combined fluorescence AFM. To generate AFM topographs adhesions, REF52 (rat embryo fibroblast) cells expressing YFP-paxillin as a marker adhesions were de-roofed paxillin-positive subsequently imaged The improved resolution...
Abstract Cadherin receptors have a well-established role in cell–cell adhesion, cell polarization and differentiation. However, some cadherins also promote tissue movement during embryonic development tumour progression. In particular, cadherin-11 is upregulated inflammatory invasion, but the mechanisms underlying stimulated migration are still incompletely understood. Here, we show that localizes to focal adhesions promotes adhesion fibronectin Xenopus neural crest, highly migratory...
Abstract Transmembrane protein 16 F (TMEM16F) is a Ca 2+ -activated homodimer which functions as an ion channel and phospholipid scramblase. Despite the availability of several TMEM16F cryogenic electron microscopy (cryo-EM) structures, mechanism activation substrate translocation remains controversial, possibly due to restrictions in accessible conformational space. In this study, we use atomic force under physiological conditions reveal range structurally mechanically diverse assemblies,...
The establishment of cell adhesion involves specific recognition events between individual cell-surface receptors and molecules the cellular environment. However, characterizing single-molecule in context a living presents an experimental challenge. atomic force microscope (AFM) operated spectroscopy mode provides ultrasensitive method to investigate forces at level single receptor-ligand bonds. With attached AFM cantilever, number cell-substrate interactions can be controlled limited...
High-speed atomic force microscopy (HS-AFM) is a powerful tool for visualizing the dynamics of individual biomolecules. However, in single-molecule HS-AFM imaging applications, x,y-scanner ranges are typically restricted to few hundred nanometers, preventing overview observation larger molecular assemblies, such as 2-dimensional protein crystal growth or fibrillar aggregation. Previous advances scanner design using mechanical amplification piezo-driven x,y-positioning system have extended...
ABSTRACT S100A11 is a small Ca2+-activatable protein known to localize along stress fibers (SFs). Analyzing localization in HeLa and U2OS cells further revealed enrichment at focal adhesions (FAs). Strikingly, levels FAs increased sharply, yet transiently, just before FA disassembly. Elevating intracellular Ca2+ with ionomycin stimulated both recruitment subsequent However, pre-incubation the non-muscle myosin II (NMII) inhibitor blebbistatin or an of stretch-activatable channel Piezo1...
Receptor-linked class I phosphoinositide 3-kinases (PI3Ks) induce assembly of signal transduction complexes through protein-protein and protein-lipid interactions that mediate cell proliferation, survival, migration. Although II PI3Ks have the potential to make same phosphoinositides as PI3Ks, their precise cellular role is currently unclear. In this report, we demonstrate 3-kinase C2beta (PI3KC2beta) associates with Eps8/Abi1/Sos1 complex recruited EGF receptor part a multiprotein signaling...
The biofunctionalization of materials creates interfaces on which proteins, cells, or tissues can fulfill native desired tasks. Here we report how to control the assembly type I collagen into well-defined nanoscopic matrices different patterns. Collagen fibrils in these ultrathin (approximately 3 nm) maintained their structure as observed vivo. This opens up possibility create programmable biofunctionalized using collagen-binding proteins fused with domains. Applied eukaryotic nanostructured...