A5070609220- Erythrocyte Function and Pathophysiology
- Ion channel regulation and function
- Cellular Mechanics and Interactions
- Ion Channels and Receptors
- Spaceflight effects on biology
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- 3D Printing in Biomedical Research
- Fermentation and Sensory Analysis
- Osteoarthritis Treatment and Mechanisms
- Exercise and Physiological Responses
- Connexins and lens biology
- Horticultural and Viticultural Research
- Axon Guidance and Neuronal Signaling
- Plant and Biological Electrophysiology Studies
- Cellular transport and secretion
- Caveolin-1 and cellular processes
- Collagen: Extraction and Characterization
- Wnt/β-catenin signaling in development and cancer
- Ion Transport and Channel Regulation
- Space Exploration and Technology
- Fungal and yeast genetics research
- Neuroscience of respiration and sleep
- Cardiomyopathy and Myosin Studies
- Microfluidic and Bio-sensing Technologies
UNSW Sydney
2016-2025
EMBL Australia
2016-2023
Max Delbrück Center
2007-2023
ARC Centre of Excellence in Advanced Molecular Imaging
2017-2020
Cellular Therapeutics (United Kingdom)
2020
The University of Adelaide
2002-2012
SENTECH Instruments (Germany)
2006
TU Dresden
2004-2005
Max Planck Institute of Molecular Cell Biology and Genetics
2002-2004
Max Planck Institute for Medical Research
1991-1999
In sensory neurons, mechanotransduction is sensitive, fast and requires mechanosensitive ion channels. Here we develop a new method to directly monitor at defined regions of the cell-substrate interface. We show that molecular-scale (~13 nm) displacements are sufficient gate currents in mouse touch receptors. Using neurons from knockout mice, displacement thresholds increase by one order magnitude absence stomatin-like protein 3 (STOML3). Piezo1 founding member class mammalian...
The joints of mammals are lined with cartilage, comprised individual chondrocytes embedded in a specialized extracellular matrix. Chondrocytes experience complex mechanical environment and respond to changing loads order maintain cartilage homeostasis. It has been proposed that mechanically gated ion channels functional importance chondrocyte mechanotransduction; however, direct evidence current activation these cells lacking. We have used high-speed pressure clamp elastomeric pillar arrays...
Abstract Neuromodulation tools are useful to decipher and modulate neural circuitries implicated in functions diseases. Existing electrical chemical cannot offer specific modulation while optogenetics has limitations for deep tissue interfaces, which might be overcome by miniaturized optoelectronic devices the future. Here, a 3D magnetic hyaluronic hydrogel is described that offers noninvasive neuromodulation via magnetomechanical stimulation of primary dorsal root ganglion (DRG) neurons....
Touch perception is enabled by mechanically activated ion channels, the opening of which excites cutaneous sensory endings to initiate sensation. In this study, we identify ELKIN1 as an channel likely gated mechanical force, necessary for normal touch sensitivity in mice. insensitivity
In conditions of microgravity the human body undergoes extensive alterations in physiological function. However, it has proven challenging to determine how these changes are mediated at molecular and cellular level. Here, we investigated whether ELKIN1, a mechanically activated ion channel, regulates structures simulated microgravity. Deletion ELKIN1 inhibited microgravity-induced structure attachment. addition, cells lacking did not exhibit focal adhesion redistribution YAP1 transcription...
Mechanoelectrical transduction is a cellular signalling pathway where physical stimuli are converted into electro-chemical signals by mechanically activated ion channels. We describe here the presence of currents in melanoma cells that dependent on TMEM87a, which we have renamed Elkin1. Heterologous expression this protein PIEZO1-deficient cells, exhibit no baseline mechanosensitivity, sufficient to reconstitute currents. Melanoma lacking functional Elkin1 defective mechanoelectrical...
Abstract Glioblastoma multiforme (GBM) is one of the most aggressive malignant brain tumors and urgently requires development new therapeutic strategies. In this study, an innovative hybrid in vitro vascularized GBM‐on‐a‐chip model presented as a strategic integration microfluidics 3D bioprinting technologies. The system can recreate compartmentalized tumor microenvironment, comprising functional blood barrier (BBB) adjacent perivascular niche, by selectively mimicking physiological shear...
PIEZO1 is a bona fide mammalian mechanically activated channel that has recently been shown to provide instructive cues during neuronal specification, texture sensing, and cell migration where mechanical inputs arise at the interface between cells their substrate. Here, we have investigated whether properties of substrate alone can modulate activity, in response exogenously applied stimuli, using elastomeric pillar arrays as force transducers. This methodology enables application stimuli...