A5101990585- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Erythrocyte Function and Pathophysiology
- Ion channel regulation and function
- Epigenetics and DNA Methylation
- Light effects on plants
- Plant and Biological Electrophysiology Studies
- Genetic and Kidney Cyst Diseases
- Ammonia Synthesis and Nitrogen Reduction
- RNA modifications and cancer
- Metalloenzymes and iron-sulfur proteins
- Fungal and yeast genetics research
- Nuclear Structure and Function
- Chemical and Physical Studies
- DNA Repair Mechanisms
Max Delbrück Center
2023-2024
National Defense Medical Center
2020-2022
Academia Sinica
1988-2022
Institute of Molecular Biology, Academia Sinica
2017-2022
Institute of Botany
1988
Touch perception is enabled by mechanically activated ion channels, the opening of which excites cutaneous sensory endings to initiate sensation. In this study, we identify ELKIN1 as an channel likely gated mechanical force, necessary for normal touch sensitivity in mice. insensitivity
Microtubule organization depends on the γ-tubulin ring complex (γ-TuRC), a ∼2.3-MDa nucleation factor comprising an asymmetric assembly of and GCP2-GCP6. However, it is currently unclear how γ-TuRC-associated microproteins MZT1 MZT2 contribute to structure regulation holocomplex. Here, we report cryo-EM structures in context native human γ-TuRC. forms two subcomplexes with N-terminal α-helical domains GCP3 or GCP6 (GCP-NHDs) within γ-TuRC "lumenal bridge." We determine X-ray recombinant...
Ran-guanosine triphosphatase orchestrates mitotic spindle assembly by modulation of the interaction between Importin-α/-β and factors (SAFs). The inhibition SAFs performed importins needs to be done without much sequestration from abundant nuclear localization signal (NLS) -containing proteins. However, molecular mechanisms that determine NLS-binding selectivity inhibit activity Importin-β-regulated (e.g., apparatus protein [NuMA]) remain undefined. Here, we present a crystal structure...
How γ-tubulin ring complex (γ-TuRC), a master template for microtubule nucleation, is spatially and temporally regulated the assembly of new arrays remains unclear. Here, we report that an evolutionarily conserved microprotein, Mozart1 (Mzt1), regulates subcellular targeting formation activity γ-TuRC at different cell cycle stages. Crystal structures protein complexes demonstrate Mzt1 promiscuously interacts with N-terminal domains multiple subunits in via intercalative binding mode....
The primary cilium, a microtubule-based sensory organelle, undergoes cycles of assembly and disassembly that govern the cell cycle progression critical to proliferation differentiation. Although cilia has been studied extensively, molecular mechanisms underlying are less well understood. Here, we uncover γ-tubulin ring complex (γ-TuRC)-dependent pathway promotes thereby prevents formation. We further demonstrate Kif2A, kinesin motor bears microtubule-depolymerizing activity, is recruited...
Abstract The slightest touch to the skin initiates tactile perception that is almost immediate 1 . extraordinary speed of enabled by mechanically-activated ion channels, opening which excites endings sensory neurons innervating initiate sensation. Here we identify a new channel, Elkin1 2 , that, when ablated in mice, leads profound behavioural insensitivity. Touch insensitivity -/- mice was caused loss currents (MA-currents) around half all are activated light (low threshold...
Abstract A gentle touch or a painful pinch are relayed to the brain and spinal cord by rapidly conducting myelinated sensory neurons 1,2 . These possess mechanically activated ion channels like PIEZO2 ELKIN1 that can be gated small movements of substrate 3–6 This gating mechanism has been proposed involve extracellular tether proteins may transfer force from matrix channel 7 , so-called model 8 The molecular nature such tethers has, however, remained elusive. Here we identify type II...
In a diurnal light/dark regimen Synechococcus RF-1 fixes N2 at high rate but almost exclusively during the dark periods. Studying time course of nitrogenase synthesis by using chloramphenicol and nitrate revealed that re-initiation was required for nitrogen fixation to appear period. Northern blot analysis demonstrated nitrogenase-specific mRNA expressed itself only in culture growing The results proved is synthesized cyclically when organism this cyclic controlled transcription level.