A5002414796- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Photosynthetic Processes and Mechanisms
- Astronomy and Astrophysical Research
- Lipid Membrane Structure and Behavior
- Enzyme Structure and Function
- Advanced Electron Microscopy Techniques and Applications
- Scientific Research and Discoveries
- Cellular transport and secretion
- Ion channel regulation and function
- Retinal Development and Disorders
- Advanced Biosensing Techniques and Applications
- Advanced Chemical Physics Studies
- Arts, Culture, and Music Studies
- Monoclonal and Polyclonal Antibodies Research
- Spectroscopy and Quantum Chemical Studies
- Biosensors and Analytical Detection
- Erythrocyte Function and Pathophysiology
- Marine Invertebrate Physiology and Ecology
- Optical Coherence Tomography Applications
- Sociology and Education Studies
- Neurobiology and Insect Physiology Research
- Innovation, Technology, and Society
- Advanced biosensing and bioanalysis techniques
- Photocathodes and Microchannel Plates
Leibniz-Forschungsinstitut für Molekulare Pharmakologie
2013-2016
Berlin Heart (Germany)
2015
Freie Universität Berlin
2011-2015
Leibniz Association
2014
Candy’s Place
2014
Bielefeld University
2011
Single molecule-based super-resolution methods have become important tools to study nanoscale structures in cell biology. However, the complexity of multi-colour applications has prevented them from being widely used amongst biologists. Direct stochastic optical reconstruction microscopy (dSTORM) offers a simple way perform single molecule imaging without need for an activator fluorophore and compatible with many conventionally fluorophores. The search ideal dye pairs suitable dual-colour...
Neurotransmission involves the exo-endocytic cycling of synaptic vesicles (SVs) within nerve terminals. Exocytosis is facilitated by a cytomatrix assembled at active zone (AZ). The precise spatial and functional relationship between exocytic fusion SVs AZ membranes endocytic SV retrieval unknown. Here, we identify scaffold G protein coupled receptor kinase 2 interacting (GIT) as component AZ-associated regulator endocytosis. GIT1 its D. melanogaster ortholog, dGIT, are shown to directly...
G protein-coupled receptors (GPCRs) represent the most important drug targets. Although smallest functional unit of a GPCR is monomer, it became clear in past decades that vast majority form dimers. Only very recently, however, data were presented some may fact be expressed as mixture monomers and dimers interaction receptor protomers dynamic. To date, equilibrium measurements restricted to plasma membrane due experimental limitations. We have addressed question where this established for...
Multicolor single molecule localization-based super-resolution microscopy (SMLM) approaches are challenged by channel crosstalk and errors in multi-channel registration. We recently introduced a spectral demixing-based variant of direct stochastic optical reconstruction (SD-dSTORM) to perform multicolor SMLM with minimal color crosstalk. Here, we demonstrate that the demixing procedure is inherently free registration therefore does not require alignment. Furthermore, significantly reduces...
Spectral demixing (SD) offers multicolor single molecule localization microscopy (SMLM) with low crosstalk and without the need to correct for registration errors. Here, we present SDmixer, a versatile, open-source software tool that enables any laboratory perform rapid SD-based SMLM. A graphic user interface allows non-experts process 2D or 3D data sets from SML reconstruct super-resolved images flexible output options.
The Energy Recovery Linac (ERL) paradigm offers the promise to generate intense electron beams of superior quality with extremely small six-dimensional phase space for many applications in physical sciences, materials science, chemistry, health, information technology and security. Helmholtz-Zentrum Berlin started 2010 an intensive R\&D programme address challenges related ERL as driver future light sources by setting up bERLinPro (Berlin Project) 50 MeV beam energy high average current....
The sensitive and rapid detection of pathogenic DNA is tremendous importance in the field diagnostics. We demonstrate ability detecting quantifying single- double-stranded with picomolar sensitivity a bead-based fluorescence assay. Selecting appropriate capturing sequences enables (2 h) reliable quantification. show that synthetic <i>S. pneumoniae</i> <i>M. luteus</i> can be quantified very small sample volumes (20 μL) across linear range over four orders magnitude from 1 nM to pM, using...