A5069347195- Olfactory and Sensory Function Studies
- Neurobiology and Insect Physiology Research
- Biochemical Analysis and Sensing Techniques
- Advanced Chemical Sensor Technologies
- Mitochondrial Function and Pathology
- Chemical Reactions and Isotopes
- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Nuclear Physics and Applications
- Retinal Development and Disorders
- Science, Research, and Medicine
- Nicotinic Acetylcholine Receptors Study
- Neuroscience and Neural Engineering
- Insect Pheromone Research and Control
- Neuroendocrine regulation and behavior
- bioluminescence and chemiluminescence research
- Neurogenesis and neuroplasticity mechanisms
- Lipid Membrane Structure and Behavior
- Gene Regulatory Network Analysis
- Essential Oils and Antimicrobial Activity
- Extracellular vesicles in disease
- Orthodontics and Dentofacial Orthopedics
- Neurological diseases and metabolism
Scuola Internazionale Superiore di Studi Avanzati
2015-2024
Sapienza University of Rome
2024
Italian Institute of Technology
2009-2012
Instituto di Biofisica
1990-2001
Istituto Nazionale per la Fisica della Materia
1996
University of Genoa
1987-1995
Istituto Centrale per la Ricerca Scientifica e Tecnologica Applicata al Mare
1990
Duke University Hospital
1990
Duke Medical Center
1990
Fidia Farmaceutici (Italy)
1986
Abstract Recent anecdotal and scientific reports have provided evidence of a link between COVID-19 chemosensory impairments, such as anosmia. However, these downplayed or failed to distinguish potential effects on taste, ignored chemesthesis, generally lacked quantitative measurements. Here, we report the development, implementation, initial results multilingual, international questionnaire assess self-reported quantity quality perception in 3 distinct modalities (smell, chemesthesis) before...
Abstract In a preregistered, cross-sectional study, we investigated whether olfactory loss is reliable predictor of COVID-19 using crowdsourced questionnaire in 23 languages to assess symptoms individuals self-reporting recent respiratory illness. We quantified changes chemosensory abilities during the course illness 0–100 visual analog scales (VAS) for participants reporting positive (C19+; n = 4148) or negative (C19−; 546) laboratory test outcome. Logistic regression models identified...
Abstract This study prospectively assessed the 6-month prevalence of self-reported and psychophysically measured olfactory dysfunction in subjects with mild-to-moderate COVID-19. Self-reported smell or taste impairment was evaluated by SNOT-22 at diagnosis, 4-week, 8-week, 6-month. At 6 months from psychophysical evaluation function also performed using 34-item culturally adapted University Pennsylvania Smell Identification Test (CA-UPSIT). 145 completed both subjective evaluation. According...
1. Olfactory receptor cells were isolated from the adult tiger salamander Ambystoma tigrinum and current in response to odorant stimuli was measured with whole‐cell voltage‐clamp technique while odorants at known concentrations rapidly applied for controlled exposure times. 2. Three odorants, cineole, isoamyl acetate acetophenone, first 5 x 10(‐4) M. Out of forty‐nine tested, 53% responded one only, 22% two 25% all three odorants. 3. The amplitude a given concentration found be dependent on...
Using an age and gender matched-pair case-control study, we aimed to estimate the long-term prevalence of psychophysical olfactory, gustatory , chemesthesis impairment at least one year after SARS-CoV-2 infection considering background chemosensory dysfunction in non-COVID-19 population.This case-controlled study included 100 patients who were home-isolated for mildly symptomatic COVID-19 between March April 2020. One control regularly tested always negative was matched each case according...
The aim of this study was to psychophysically evaluate the prevalence smell and taste dysfunction 2 years after mildly symptomatic severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) infection compared that observed at 1-year follow-up while considering background chemosensory in no-coronavirus disease 2019 (COVID-19) population.This is a prospective case-control on 93 patients with polymerase chain reaction (PCR)-positive SARS-CoV-2 matched controls. Self-reported olfactory...
Sensory transduction shares common features in widely different sensory modalities. The purpose of this article is to examine the similarities and differences underlying mechanisms receptor cells for vision, olfaction, hearing. One major between systems relates nature stimulus. In both visual olfactory a quantal mechanism detection possible, because absorption photon or binding an odorant molecule provides energy change significantly greater than thermal noise molecule. hearing, on other...
1. The kinetics of photoresponses to flashes and steps light rods, from the retina newt Triturus cristatus, were analysed by recording membrane current with a suction electrode. 2. In dark‐adapted conditions relation between normalized amplitude photoresponse at fixed time 1 s after onset intensity could be fitted an exponential or polynomial relation. presence steady bright same Michaelis‐Menten 3. stimuli was reconstructed using model in which: (i) three molecules guanosine 3'.5'‐cyclic...
Ca-activated Cl channels are an important component of olfactory transduction. Odor binding to receptors in the cilia sensory neurons (OSNs) leads increase intraciliary Ca concentration by entry through cyclic nucleotide-gated (CNG) channels. activates a channel that efflux from cilia, contributing amplification OSN depolarization. The molecular identity this remains elusive. Recent evidence has indicated bestrophins able form heterologous systems. Here we have analyzed expression mouse...
The aim of this study was to investigate, over a period approximately 3 years, the reactions orthodontic loading type V titanium miniscrew. In retrospective study, conducted in private practice, records 300 miniscrews inserted 132 consecutive patients (80 females, 60.6 percent) by same surgeon were evaluated. mean age 23.2 years. Three types (type A: diameter 1.5 mm, length 9 mm; B: 11 and C: 1.3 mm) used. clinical variables evaluated included time location miniscrew relation gingiva root....
The Ca2+-activated Cl− channel TMEM16B is highly expressed in the cilia of olfactory sensory neurons (OSNs). Although a large portion odor-evoked transduction current carried by channels, their role olfaction still controversial. A previous report (Billig et al. 2011. Nat. Neurosci. http://dx.doi.org/10.1038/nn.2821) showed that disruption TMEM16b/Ano2 gene mice abolished currents OSNs but did not produce any major change behavior. Here we readdress and show knockout (KO) have behavioral...
Abstract Transmembrane protein 16 F (TMEM16F) is a Ca 2+ -activated homodimer which functions as an ion channel and phospholipid scramblase. Despite the availability of several TMEM16F cryogenic electron microscopy (cryo-EM) structures, mechanism activation substrate translocation remains controversial, possibly due to restrictions in accessible conformational space. In this study, we use atomic force under physiological conditions reveal range structurally mechanically diverse assemblies,...
1. Blockage and permeation of divalent cations through channels activated by guanosine 3',5'‐cyclic monophosphate (cyclic GMP) were studied in membrane patches excised from retinal rods the tiger salamander Ambystoma tigrinum rapidly changing ionic medium bathing intracellular side membrane. 2. The Na+ current, observed when 110 mM‐NaCl was present on both sides patch, reduced addition 1 mM chloride salts Ca2+, Mg2+, Sr2+, Ba2+ or Mn2+ to medium. sequence blocking potency at +60 mV Mg2+...
The rodent vomeronasal organ plays a crucial role in several social behaviors. Detection of pheromones or other emitted signaling molecules occurs the dendritic microvilli sensory neurons, where binding to receptors leads influx sodium and calcium ions mainly through transient receptor potential canonical 2 (TRPC2) channel. To investigate physiological played by increase intracellular concentration apical region these we produced localized, rapid, reproducible increases with flash photolysis...
Ca(2+)-activated Cl(-) channels play relevant roles in several physiological processes, including olfactory transduction, but their molecular identity is still unclear. Recent evidence suggests that members of the transmembrane 16 (TMEM16, also named anoctamin) family form cell types. In vertebrate TMEM16b/anoctamin2 has been proposed as major component channels. However, a comparison functional properties whole-cell configuration between native and candidate channel not yet performed. this...
The innate immune system is crucial in fighting SARS-CoV-2 infection, which responsible for coronavirus disease 2019 (COVID-19). Therefore, deepening our understanding of the underlying response mechanisms fundamental development novel therapeutic strategies. role extra-oral bitter (TAS2Rs) and sweet (TAS1Rs) taste receptors regulation has yet to be fully understood. However, a few studies have investigated association between receptor genes COVID-19 symptom severity, with controversial...