A5014143406- Mitochondrial Function and Pathology
- Chemical Reactions and Isotopes
- Cardiac electrophysiology and arrhythmias
- Nuclear Physics and Applications
- Science, Research, and Medicine
- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Extracellular vesicles in disease
- Olfactory and Sensory Function Studies
- Neural dynamics and brain function
- Neurobiology and Insect Physiology Research
- MicroRNA in disease regulation
- Advanced Chemical Sensor Technologies
- stochastic dynamics and bifurcation
- Force Microscopy Techniques and Applications
- Ferroelectric and Negative Capacitance Devices
- Receptor Mechanisms and Signaling
- Advanced Electron Microscopy Techniques and Applications
Scuola Internazionale Superiore di Studi Avanzati
2022-2024
Universidad Nacional de Colombia
2022
Abstract Transmembrane protein 16 F (TMEM16F) is a Ca 2+ -activated homodimer which functions as an ion channel and phospholipid scramblase. Despite the availability of several TMEM16F cryogenic electron microscopy (cryo-EM) structures, mechanism activation substrate translocation remains controversial, possibly due to restrictions in accessible conformational space. In this study, we use atomic force under physiological conditions reveal range structurally mechanically diverse assemblies,...
Abstract Seizures represent a frequent symptom in gliomas and significantly impact patient morbidity quality of life. Although the pathogenesis tumor-related seizures is not fully understood, accumulating evidence indicates key role peritumoral microenvironment. Brain cancer cells interact with neurons by forming synapses them releasing exosomes, cytokines, other small molecules. Strong interactions among often lead to synchronization their activity. In this paper, we used an vitro model...
Patients affected by glioma frequently experience epileptic discharges; however, the causes of brain tumor-related epilepsy (BTRE) are still not completely understood. We investigated mechanisms underlying BTRE analyzing effects exosomes released U87 cells and patient-derived cells. Rat hippocampal neurons incubated for 24 hours with these exhibited increased spontaneous firing, while their resting membrane potential shifted positively 10-15 mV. Voltage clamp recordings demonstrated that...
The COVID-19 pandemic brought attention to our limited understanding of human olfactory physiology. While the cellular composition epithelium is similar that other vertebrates, its functional properties are largely unknown. We prepared acute slices from nasal biopsies and used whole-cell patch-clamp technique record electrical cells. measured voltage-gated currents in sensory neurons supporting cells, action potentials neurons. Additionally, neuronal inward current responses a...
Abstract Glioblastoma (GBM) is amongst the deadliest types of cancers, with no resolutive cure currently available. GBM cell proliferation in patient’s brain a complex phenomenon controlled by multiple mechanisms. The aim this study was to determine whether ionic fluxes controlling duplication could represent target for therapy. In work, we combined multi-channel Ca2+ and Cl− imaging, optical tweezers, electrophysiology, immunohistochemistry describe role ion mediating volume changes that...
<p>Synchronization of Ca<sup>2+</sup> flares upon Yoda1 addition.</p>
<p>VRAC-like currents are observed in patient-derived GSCs.</p>
<p>Cl- signals and large Ca<sup>2+</sup> flares in round cells.</p>
<p>Mitosis of a U87 cell visualized through the NucBlue dye.</p>
<p>Comparison of electrical properties flat and rounded U87 cells.</p>
<p>Glioma cells experience changes in volume due to Cl- currents and associated water movements.</p>
<p>The addition of Yoda 1 on patient-derived GSCs had a similar effect to that described for U87 cells, but the ratio between amplitude Ca<sup>2+</sup> and Cl- flares, kinetics Ca<sup>2+</sup>coupled Cl<sup>−</sup> responses varied in different patients.</p>
<p>Comparison of electrophysiological currents with CsCl- and KCl-based intracellular solutions.</p>
<p>Toxicity analysis of NFA and CBX on U87 cells.</p>
<p>Expression of p53 in U87 cells increases after treatment with NFA and CBX.</p>
<p>Change of volume during the transition from metaphase to telophase.</p>
<p>Flow cytometry analysis of cell cycle phases for NFA- and CBX-treated U87 cells.</p>
<p>Spontaneous, asynchronous Ca<sup>2+</sup> flares in U87 cells becomes synchronous upon addition of the Piezo1 activator Yoda1.</p>
<p>Histochemical analysis of TMEM16A (A), TMEM16B (B), VRAC (C) and Piezo 1 (D) channels in flat / rounded U87 cells.</p>
<p>Toxicity and functional assays on mouse cortical neurons upon treatment with increasing concentrations of NFA CBX.</p>
<p>Flow cytometry analysis of cell cycle phases for NFA- and CBX-treated U87 cells.</p>
<div>Abstract<p>Glioblastoma (GBM) is amongst the deadliest types of cancers, with no resolutive cure currently available. GBM cell proliferation in patient’s brain a complex phenomenon controlled by multiple mechanisms. The aim this study was to determine whether ionic fluxes controlling duplication could represent target for therapy. In work, we combined multi-channel Ca<sup>2+</sup> and Cl<sup>−</sup> imaging, optical tweezers, electrophysiology,...
<p>Spontaneous Ca<sup>2+</sup>-activated Cl- flares.</p>
<p>Toxicity and functional assays on mouse cortical neurons upon treatment with increasing concentrations of NFA CBX.</p>
<p>Cl- signals and large Ca<sup>2+</sup> flares in round cells.</p>