A5040108245- Orthopaedic implants and arthroplasty
- Orthopedic Infections and Treatments
- Total Knee Arthroplasty Outcomes
- Advanced materials and composites
- Advanced biosensing and bioanalysis techniques
- Ion channel regulation and function
- Spectroscopy Techniques in Biomedical and Chemical Research
- Force Microscopy Techniques and Applications
- Tribology and Wear Analysis
- Bone Tissue Engineering Materials
- Metal and Thin Film Mechanics
- Ion Transport and Channel Regulation
- Analytical Chemistry and Sensors
- Polymer crystallization and properties
- Advanced ceramic materials synthesis
- Catalytic Cross-Coupling Reactions
- Lipid Membrane Structure and Behavior
- Neuroscience and Neuropharmacology Research
- Polymer Nanocomposite Synthesis and Irradiation
- Mesoporous Materials and Catalysis
- Neurobiology and Insect Physiology Research
- Ion Channels and Receptors
- Photopolymerization techniques and applications
- Infrared Thermography in Medicine
- Thermography and Photoacoustic Techniques
Ca' Foscari University of Venice
2023-2025
Life Science Institute
2020-2023
Kanazawa University
2020-2023
Kyoto Pharmaceutical University
2022
Kyoto Prefectural University of Medicine
2014-2021
Kyoto Institute of Technology
2007-2018
University of Missouri
2018
Institute of Science and Technology for Ceramics
2008
Organisms of Gram-negative phylum bacteroidetes, Porphyromonas gingivalis, underwent lysis on polished surfaces silicon nitride (Si3N4) bioceramics. The antibacterial activity Si3N4 was mainly the result chemically driven principles. lytic activity, although not osmotic in nature, related to peculiar pH-dependent surface chemistry Si3N4. A buffering effect via formation ammonium ions (NH4(+)) (and their modifications) experimentally observed by pH microscopy. Lysis confirmed conventional...
Abstract Transmembrane protein 16 F (TMEM16F) is a Ca 2+ -activated homodimer which functions as an ion channel and phospholipid scramblase. Despite the availability of several TMEM16F cryogenic electron microscopy (cryo-EM) structures, mechanism activation substrate translocation remains controversial, possibly due to restrictions in accessible conformational space. In this study, we use atomic force under physiological conditions reveal range structurally mechanically diverse assemblies,...
The remarkable stoichiometric flexibility of hydroxyapatite (HAp) enables the formation a variety charged structural sites at material's surface which facilitates bone remodeling due to binding biomolecule moieties in zwitterionic fashion. In this paper, we report for first time that an optimized biomedical grade silicon nitride (Si3N4) demonstrated cell adhesion and improved osteoconductivity comparable highly defective, non-stoichiometric natural hydroxyapatite. Si3N4's zwitterionic-like...
Abstract Regulation of intracellular pH is critically important for many cellular functions. The quantification proton extrusion in different types cells and physiological conditions pivotal to fully elucidate the mechanisms homeostasis. Here we show use gold nanoparticles (AuNP) create a high spatial resolution sensor measuring extracellular proximity cell membrane. We test on HepG2 liver cancer MKN28 gastric before after inhibition Na + /H exchanger. surface conjugation strategy conceived...
Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) plays a pivotal role in synaptic plasticity. It is dodecameric serine/threonine that has been highly conserved across metazoans for over million years. Despite the extensive knowledge of mechanisms underlying CaMKII activation, its behavior at molecular level remained unobserved. In this study, we used high-speed atomic force microscopy to visualize activity-dependent structural dynamics rat/hydra/ C. elegans with nanometer resolution....
Nucleosome dynamics, such as nucleosome sliding and DNA unwrapping, are important for gene regulation in eukaryotic chromatin. H2A.Z, a variant of histone H2A that is highly evolutionarily conserved, participates by forming unstable multipositioned nucleosomes vivo vitro. However, the subsecond dynamics this have not been directly visualized under physiological conditions. Here, we used high-speed atomic force microscopy (HS-AFM) to visualize human H2A.Z.1-nucleosomes. HS-AFM videos show...
Raman spectroscopy is used for the characterization of two main mechanisms responsible degradation acetabular cups in hip joints: creep deformation and oxidation. The term refers to permanent that occurs under effect body weight does not completely recover after load release. This mechanism involves no mass loss from sample, but packing adjustment polyethylene molecules their reciprocal positions pressure. Conversely, oxidation triggers wear, which accompanied by irreversible material:...
The articulation performance of silicon nitride against conventional and highly cross-linked polyethylene, as well for self-mated bearings, was examined in a series standard hip simulation studies. Wear rates polyethylene liners femoral heads were consistent with reported literature, although higher than cobalt chromium controls. Excessive protein precipitation confounding factor interpretation the wear data. Post wear-test Raman spectroscopy showed no oxidative degradation. found to be...
Confocal spectroscopic techniques are applied to selected Raman bands study the microscopic features of acetabular cups made ultra-high molecular weight polyethylene (UHMWPE) before and after implantation in vivo. The micrometric lateral resolution a laser beam focused on polymeric surface (or subsurface) enables highly resolved visualization 2-D conformational population patterns, including crystalline, amorphous, orthorhombic phase fractions, oxidation index. An optimized confocal probe...
NaBiF4 nanocrystalline particles were synthesized by means of a facile precipitation synthesis route to explore upconversion emission properties when doped with lanthanide ions. In particular, the incorporation Yb3+-Ho3+-Ce3+ triad controlled ion concentration facilitates near-IR pumping conversion into visible light, possibility color tuning depending on Ce3+ doping amount. We observed that introducing content up 20 at.% in NaBiF4:Yb3+/Ho3+, chromaticity progressively turns from green for...
Wear of polyethylene acetabular cups in patients total hip arthroplasty is routinely deduced from the penetration femoral head into liner as observed radiographs. However, linear thus measured represents cumulative contribution two components, one due to wear, and other creep or irreversible deformation structure. The erroneous attribution wear entire displacement cup might lead misinterpretation actual performance cups. aim this study was quantify components creep, they occur vivo cups,...
Abstract We applied a Raman confocal spectroscopic technique to quantitatively assess the structural features of two kinds acetabular cups made ultra‐high molecular weight polyethylene. wanted know whether polyethylene belonging different generations, and thus manufactured by procedures, possess structures how those differences affected their wear resistance. Emphasis was placed on oxidation profiles developed along cross‐sectional depth in main zone during testing hip simulator. The...
The typical response to the lowering of plasma Na+ concentration and blood pressure in our body involves release aldosterone from adrenal glands, which triggers reabsorption sodium kidney. Although effects on this physiological mechanism were extensively studied past decades, there are still some aspects be fully elucidated. In present study, we propose for first time a new approach based Raman spectroscopy monitor ionic activity aldosterone-treated A6 renal epithelial cells. This...