A5075540271- Bone Tissue Engineering Materials
- Dental materials and restorations
- Spectroscopy Techniques in Biomedical and Chemical Research
- Dental Implant Techniques and Outcomes
- Semiconductor materials and devices
- Orthopaedic implants and arthroplasty
- Antifungal resistance and susceptibility
- Electrospun Nanofibers in Biomedical Applications
- Oral microbiology and periodontitis research
- Orthopedic Infections and Treatments
- Dental Health and Care Utilization
- Bacterial biofilms and quorum sensing
- Graphene and Nanomaterials Applications
- Spectroscopy and Chemometric Analyses
- Advancements in Semiconductor Devices and Circuit Design
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Additive Manufacturing and 3D Printing Technologies
- Corneal Surgery and Treatments
- Mesenchymal stem cell research
- Bacterial Identification and Susceptibility Testing
- Ferroelectric and Negative Capacitance Devices
- Dental Erosion and Treatment
- Synthesis and properties of polymers
- Antimicrobial agents and applications
Kyoto Prefectural University of Medicine
2015-2024
Kansai Medical University
2023-2024
Kyoto Prefectural Education Center
2022-2024
SINTX Technologies (United States)
2018
Graduate School USA
2018
Osaka Dental University
2012-2017
National Institute for Materials Science
2007-2011
Toyo University
2005
Kyoto Institute of Technology
1999
Osaka University
1990
Abstract While the reciprocity between bioceramics and living cells is complex, it principally governed by implant’s surface chemistry. Consequently, a deeper understanding of chemical interactions with tissue could ultimately lead to new therapeutic strategies. However, physical principles that govern these remain unclear. The intricacies this biological synergy are explored within paper examining peculiar chemistry relatively bioceramic, silicon nitride (Si 3 N 4 ). Building upon prior...
Organisms of Gram-negative phylum bacteroidetes, Porphyromonas gingivalis, underwent lysis on polished surfaces silicon nitride (Si3N4) bioceramics. The antibacterial activity Si3N4 was mainly the result chemically driven principles. lytic activity, although not osmotic in nature, related to peculiar pH-dependent surface chemistry Si3N4. A buffering effect via formation ammonium ions (NH4(+)) (and their modifications) experimentally observed by pH microscopy. Lysis confirmed conventional...
Polyetheretherketone (PEEK) is a popular polymeric biomaterial which primarily used as an intervertebral spacer in spinal fusion surgery; but it developed for trauma, prosthodontics, maxillofacial, and cranial implants. It has the purported advantages of elastic modulus similar to native bone can be easily formed into custom 3D shapes. Nevertheless, PEEK's disadvantages include its poor antibacterial resistance, lack bioactivity, radiographic transparency. This study presents simple approach...
The thermally sensitive polymer, poly (l-lactic acid) PLLA, is first printed to a high-resolution object using an advanced 3D printing technology, called melt electrowriting (MEW). MEW processing conditions are systematically investigated manufacture PLLA scaffolds with adjustable filament diameter and pore size for the application of bone tissue engineering. To this end, relationships among rheological behavior molten electro-hydrodynamics jet, parameters (applied voltage, collection speed,...
Abstract The multiple mutations of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) virus have created variants with structural differences in both their spike and nucleocapsid proteins. While functional relevance these is under continuous scrutiny, current findings documented detrimental impact terms affinity host receptors, antibody resistance, diagnostic sensitivity. Raman spectra collected on two British variant sub‐types found Japan (QK002 QHN001) are compared that...
RNAi enables potent and specific gene silencing, potentially offering useful means for treatment of cancers. However, safe efficient drug delivery systems (DDS) that are appropriate intra-tumor siRNA or shRNA have rarely been established, hindering clinical application technology to cancer therapy. We devised hydrogel polymer nanoparticles, nanogel, shown its validity as a novel DDS various molecules. Here we examined the potential self-assembled nanogel cholesterol-bearing cycloamylose with...
The remarkable stoichiometric flexibility of hydroxyapatite (HAp) enables the formation a variety charged structural sites at material's surface which facilitates bone remodeling due to binding biomolecule moieties in zwitterionic fashion. In this paper, we report for first time that an optimized biomedical grade silicon nitride (Si3N4) demonstrated cell adhesion and improved osteoconductivity comparable highly defective, non-stoichiometric natural hydroxyapatite. Si3N4's zwitterionic-like...
Osteosarcoma cell viability, proliferation, and differentiation into osteoblasts on a silicon nitride bioceramic were examined as function of chemical modifications its as-fired surface. Biological spectroscopic analyses showed that (i) postsintering annealing in N2 gas significantly improved apatite formation from human osteosarcoma (SaOS-2) cells; (ii) situ Raman monitoring revealed new metabolic details the SaOS-2 cells, including fine differences intracellular RNA membrane phospholipids;...
This study investigated the efficiency of chitosan/polyethylene oxide (PEO)-based nanofibers with incorporated bioactive glass particles as a coating for titanium alloy, in order to improve bacteriostatic behavior and, concurrently, promote production mineralized tissue. Nanofibers and without bioglass powder were fabricated by electrospinning technique characterized using several microscopic spectroscopic techniques their morphological physiochemical properties. Subsequently, substrates...
Oral candidiasis, a common opportunistic infection of the oral cavity, is mainly caused by following four
High-precision poly(L-lactic acid) (PLLA) scaffold fabricated by melt electrowriting (MEW) as a bone substitute was investigated, however, the inherent fragility and low bioactivity of impede its clinical application. In this context, biomimetic reinforced composite combining MEW PLLA lattice with gelatin/genipin/bioglass hydrogel is firstly proposed. Incorporating 1 wt% bioglass, shows excellent interconnectivity, high storage modulus (121.3 kPa, 1.3 times gelatin/genipin) improved (422.3...
Raman spectroscopy proved why the antibacterial response of non-oxide Si 3 N 4 bioceramic is superior to those alumina-based oxide bioceramics.
The hydrolytic processes occurring at the surface of silicon nitride (Si3N4) bioceramic have been indicated as a powerful pathway to instantaneous inactivation SARS-CoV-2 virus. However, virus mechanisms promoted by Si3N4 remain yet be elucidated. In this study, we provide evidence damage incurred on upon contact with Si3N4. We also emphasize safety characteristics for mammalian cells. Contact between virions and micrometric particles immediately targeted variety viral molecules inducing...
This study targets on-site/real-time taxonomic identification and metabolic profiling of seven different Candida auris clades/subclades by means Raman spectroscopy imaging. Representative spectra from samples were systematically deconvoluted a customized machine-learning algorithm linked to database in order decode structural differences at the molecular scale. analyses metabolites revealed clear cell walls membrane structure among clades/subclades. Such are key maintaining integrity...
Zirconia is the most common ceramic used in dental implants. Even if it possesses both mechanical strength and esthetics, its intrinsic bio-inertness often results a lack of biological integration. On other hand, Si3N4 has been proved to be bio-active able easily osteointegrate. In this work, powder-based laser-cladding process developed order improve response biomedical zirconia. The resulted formation composite coating with particles dispersed nano-crystalline/amorphous silicon....
The aim of this study was to elucidate the chemistry cellular degeneration in human neuroblastoma cells upon exposure outer-membrane vesicles (OMVs) produced by Porphyromonas gingivalis (Pg) oral bacteria monitoring their metabolomic evolution using situ Raman spectroscopy. Pg-OMVs are a key factor Alzheimer's disease (AD) pathogenesis, as they act efficient vectors for delivery toxins promoting neuronal damage. However, chemical mechanisms underlying direct impact on cell metabolites at...
Staphylococcus epidermidis (S. epidermidis) is one of the leading nosocomial pathogens, particularly associated with periprosthetic infections biomedical implants. Silicon nitride (Si3N4), a nonoxide biomaterial widely used in spinal implants, has shown bacteriostatic effects against both gram-positive and gram-negative bacteria; however, physicochemical interactions between Si3N4 bacteria yet remain conspicuously unexplored. In situ time-lapse Raman spectroscopic experiments were conducted...