Selective neuronal death in the CA1 sector of hippocampus [delayed (DND)] develops several days after transient global cerebral ischemia rodents. Because NGF plays a potential role survival, it was decided to study its effect DND. We report here that intraventricular injection either before or 5 min forebrain Mongolian gerbil significantly reduced occurrence The tissue content decreased 2 d and recovered preischemic level by 1 week. By Golgi staining technique, changes first began dendrites...

A novel pH-sensitive hydrogel system consisting of poly(methacrylic acid-g-ethylene glycol) (P(MAA-g-EG)) and acryloyl group modified-cholesterol-bearing pullulan (CHPOA) nanogels was developed for the controlled delivery an anticonvulsant drug, pregabalin (PGB). Here, hydrophilic network provides swelling behavior, whereas nanogel components form separate reservoirs drugs with different hydrophobicities. These nanocarrier-integrated hybrid gels were synthesized through both...

RNAi enables potent and specific gene silencing, potentially offering useful means for treatment of cancers. However, safe efficient drug delivery systems (DDS) that are appropriate intra-tumor siRNA or shRNA have rarely been established, hindering clinical application technology to cancer therapy. We devised hydrogel polymer nanoparticles, nanogel, shown its validity as a novel DDS various molecules. Here we examined the potential self-assembled nanogel cholesterol-bearing cycloamylose with...

Abstract The decellularized cornea has received considerable attention for use as an artificial cornea. is free from cellular components and other immunogens, but maintains the integrity of extracellular matrix. However, ultrastructure yet to be demonstrated in detail. We investigated influence high hydrostatic pressure (HHP) on decellularization corneal its involvement transparency assessed vivo behaviour using two animal transplantation models, relation remodelling collagen fibrils....

The purpose of this study is to demonstrate the feasibility DALK using a decellularized corneal matrix obtained by HHP methodology. Porcine corneas were hydrostatically pressurized at 980 MPa 10°C for 10 minutes destroy cells, followed washing with EGM-2 medium remove cell debris. HHP-treated stained H-E assess efficacy decellularization. 300 μm thickness and 6.0 mm diameter was transplanted onto keratectomy wound. time course regeneration on evaluated haze grading score, fluorescein...

Abstract Following small‐diameter vascular grafting, blood vessels fail to retain excellent antithrombotic function and therefore require application of antithrombogenic drugs. We have previously reported early attachment endothelial cells the luminal surface high hydrostatic pressure (HHP)‐decellularized arteries after transplantation. In addition, graft retained by endothelialization remained open for several weeks. To fabricate tube grafts optimal size shape we evaluated decellularized...

Abstract Hyaluronic acid (HA) has garnered much attention in the development of novel hydrogels. Hydrogels, as drug delivery systems, are very important tissue engineering applications. In this study, we developed a HA nanogel containing cholesterol and maleimide derivative (HAMICH) its corresponding crosslinked hydrogel (HAMICH gel) to encapsulate drugs for their subsequent release. HAMICH gels self-assemble into nanoparticles via hydrophobic interactions. Dynamic light scattering analysis...

In spite of recent advance in understanding the stoichiometry 22-kDa human GH (22K-hGH) with cell surface hGH receptor (hGHR) and hGH-binding protein (hGH-BP) circulating plasma, that 20-kDa (20K-hGH) is poorly understood. To clarify this, mouse pro-B Ba/F3 cells stably expressing full-length hGHR (Ba/F3-hGHR) both recombinant native hGH-BP were used this study. Cell proliferation assay revealed two isoforms increased Ba/F3-hGHR to same extent a dose-dependent manner at 0.1 pm–10 nm....

Several GH isoforms have been identified in pituitary and serum, the most abundant of which is 22K human (hGH) isoform. The 20K hGH isoform produced by alternative splicing messenger ribonucleic acid comprises approximately 10% all pituitary. physiological role remains to be determined, partly because lack a simple specific assay. We established sensitive enzyme-linked immunosorbent assays (ELISAs) hGH. To determine whether regulation secretion same as that for hGH, we measured serum...

Apert syndrome is an autosomal dominantly inherited disorder caused by missense mutations in fibroblast growth factor receptor 2 (FGFR2). Surgical procedures are frequently required to reduce morphological and functional defects patients with syndrome; therefore, the development of noninvasive treat critical. Here we aimed clarify etiological mechanisms craniosynostosis mouse models verify effects purified soluble FGFR2 harboring S252W mutation (sFGFR2IIIcS252W) on calvarial sutures mice...

Recent applications of decellularized tissue have included the use hydrogels for injectable materials and three-dimensional (3D) bioprinting bioink regeneration. Microvascular formation is required delivery oxygen nutrients to support cell growth regeneration in tissues organs. The aim present study was evaluate capillary networks extracellular matrix (d-ECM) hydrogels. d-ECM were obtained from small intestine submucosa (SIS) urinary bladder (UBM) after decellularizing with sodium...

Twenty-kilodalton human GH (20K), which is one of the (hGH) variants, thought to be produced by alternative premessenger ribonucleic acid splicing. However, its physiological role still unclear due lack a specific assay. We have measured serum 20K and 22-kDa hGH (22K) ELISAs investigate in children. The subjects were 162 normal children, aged 1 month 20 yr; 12 patients with deficiency (GHD), 11 months 13 57 children non-GHD short stature, 2-17 girls Turner's syndrome, 5 15 yr. Samples...

Abstract The cell adhesion was investigated on polymer brushes of poly(2-hydroxyethyl methacrylate) (PHEMA), acrylate) (PHEA), and poly[poly(ethylene glycol) methyl ether methacrylate] (PPEGMA) with different graft densities (in semidilute concentrated regimes) thicknesses. (CPBs) all the three, regardless differences in chemical structure hydrophilicity, almost completely suppressed L929 fibroblast contrast to corresponding (SDPBs). Along our previous study excellent protein repellency CPB...

Autologous vascular grafts are widely used in revascularization surgeries for small caliber targets. However, the availability of autologous conduits might be limited due to prior or quality vessels. Xenogeneic decellularized from animals can potentially a substitute grafts. Decellularization with high hydrostatic pressure (HHP) is reported highly preserve extracellular matrix (ECM), creating feasible conditions recellularization and remodeling after implantation. In present study, we...

Most collagen matrices used in tissue engineering and regenerative medicine are gels or sponges. Our goal is to prepare an artificial extracellular matrix (ECM) that possesses similar structure the native ECM of soft order replicate its physical biological properties. Here, we report preparation with fibrillated structures based on fibrillogenesis/gelling method. We focused regenerating function tissues by creating a multifunctional collagen. A sponge/gel composite which allows cell...

Wound-dressing materials that promote wound healing while protecting wounds from infections are advantageous for clinical applications. Hence, we developed a cholesterol-bearing pullulan (CHP) nanogel stimulated healing; however, it was mechanically weak and difficult to handle. Thus, the purpose of this study examine precisely effects reinforced nanogel-crosslinked (NanoClik) membrane on healing.NanoClik prepared by mixing thiol-terminated polyethylene glycol solution an acryloyl...