A5016739752- Spectroscopy Techniques in Biomedical and Chemical Research
- SARS-CoV-2 and COVID-19 Research
- Bone Tissue Engineering Materials
- Tea Polyphenols and Effects
- SARS-CoV-2 detection and testing
- Salivary Gland Disorders and Functions
- Dental materials and restorations
- Antifungal resistance and susceptibility
- Lignin and Wood Chemistry
- Tryptophan and brain disorders
- Antimicrobial agents and applications
- RNA Interference and Gene Delivery
- Herpesvirus Infections and Treatments
- Influenza Virus Research Studies
- Heat shock proteins research
- Cardiovascular Disease and Adiposity
- CRISPR and Genetic Engineering
- Bacterial biofilms and quorum sensing
- Essential Oils and Antimicrobial Activity
- Enzyme-mediated dye degradation
- Biosensors and Analytical Detection
- Viral gastroenteritis research and epidemiology
- Spectroscopy and Chemometric Analyses
- Orthopedic Infections and Treatments
- Bacterial Identification and Susceptibility Testing
Kyoto Prefectural University of Medicine
1999-2025
Abstract SARS-CoV-2 has mutated during the global pandemic leading to viral adaptation medications and vaccinations. Here we describe an engineered human virus receptor, ACE2, by mutagenesis screening for binding receptor domain (RBD). Three cycles of random cell sorting achieved sub-nanomolar affinity RBD. Our structural data show that enhanced comes from better hydrophobic packing hydrogen-bonding geometry at interface. Additional disulfide mutations caused fixing a closed ACE2...
Potential effects of tea and its constituents on SARS-CoV-2 infection were assessed in vitro. Infectivity was decreased to 1/100 undetectable levels after a treatment with black tea, green roasted or oolong for 1 min. An addition (−) epigallocatechin gallate (EGCG) significantly inactivated SARS-CoV-2, while the same concentration theasinensin A (TSA) galloylated theaflavins including theaflavin 3,3′-di-O-gallate (TFDG) had more remarkable anti-viral activities. EGCG, TSA, TFDG at mM, 40 µM,...
Abstract The multiple mutations of the severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) virus have created variants with structural differences in both their spike and nucleocapsid proteins. While functional relevance these is under continuous scrutiny, current findings documented detrimental impact terms affinity host receptors, antibody resistance, diagnostic sensitivity. Raman spectra collected on two British variant sub‐types found Japan (QK002 QHN001) are compared that...
Abstract The coronavirus disease 2019 (COVID-19), caused by the novel severe acute respiratory syndrome 2 (SARS-CoV-2), remains to spread worldwide. COVID-19 is characterized striking high mortality in elderly; however, its mechanistic insights remain unclear. Systemic thrombosis has been highlighted pathogenesis of COVID-19, and lung microangiopathy association with endothelial cells (ECs) injury reported post-mortem analysis lungs. Here, we experimentally investigated SARS-CoV-2 infection...
This study investigated the efficiency of chitosan/polyethylene oxide (PEO)-based nanofibers with incorporated bioactive glass particles as a coating for titanium alloy, in order to improve bacteriostatic behavior and, concurrently, promote production mineralized tissue. Nanofibers and without bioglass powder were fabricated by electrospinning technique characterized using several microscopic spectroscopic techniques their morphological physiochemical properties. Subsequently, substrates...
Oral candidiasis, a common opportunistic infection of the oral cavity, is mainly caused by following four
Surface inactivation of human microbial pathogens has a long history. The Smith Papyrus (2600 ~ 2200 B.C.) described the use copper surfaces to sterilize chest wounds and drinking water. Brass bronze on doorknobs can discourage spread in hospitals, metal-base surface coatings are used hygiene-sensitive environments, both as inactivators modulators cellular immunity. A limitation these approaches is that reactive oxygen radicals (ROS) generated at metal also damage cells by oxidizing their...
The hydrolytic processes occurring at the surface of silicon nitride (Si3N4) bioceramic have been indicated as a powerful pathway to instantaneous inactivation SARS-CoV-2 virus. However, virus mechanisms promoted by Si3N4 remain yet be elucidated. In this study, we provide evidence damage incurred on upon contact with Si3N4. We also emphasize safety characteristics for mammalian cells. Contact between virions and micrometric particles immediately targeted variety viral molecules inducing...
This study targets on-site/real-time taxonomic identification and metabolic profiling of seven different Candida auris clades/subclades by means Raman spectroscopy imaging. Representative spectra from samples were systematically deconvoluted a customized machine-learning algorithm linked to database in order decode structural differences at the molecular scale. analyses metabolites revealed clear cell walls membrane structure among clades/subclades. Such are key maintaining integrity...
Raman multiomics gives unique insight into the structural characteristics of type A and B influenza viruses. Si 3 N 4 fine powder completely inactivates virus strains by a mechanism that poisons them with hydrolytic ammonia locally enhances pH.
Zirconia is the most common ceramic used in dental implants. Even if it possesses both mechanical strength and esthetics, its intrinsic bio-inertness often results a lack of biological integration. On other hand, Si3N4 has been proved to be bio-active able easily osteointegrate. In this work, powder-based laser-cladding process developed order improve response biomedical zirconia. The resulted formation composite coating with particles dispersed nano-crystalline/amorphous silicon....
Saliva plays major roles in the human-to-human transmission of SARS-CoV-2. If virus saliva SARS-CoV-2-infected individuals can be rapidly and efficiently inactivated by a beverage, ingestion beverage may attenuate spread infection within population. Recently, we reported that SARS-CoV-2 was significantly treatment with black tea, green roasted tea oolong as well their constituents, (-) epigallocatechin gallate (EGCG), theasinensin A (TSA), galloylated theaflavins. However, it remains unclear...
Abstract The Omicron subvariants of SARS-CoV-2 have multiple mutations in the S-proteins and show high transmissibility. We previously reported that tea catechin (−)-epigallocatechin gallate (EGCG) its derivatives including theaflavin-3,3’-di-O-digallate (TFDG) strongly inactivated conventional by binding to receptor domain (RBD) S-protein. Here we were effectively green tea, Matcha, black tea. EGCG TFDG suppressed infectivity BA.1 XE subvariants, while effect on BA.2.75 was weaker....
Hydrolytic reactions taking place at the surface of a silicon nitride (Si3N4) bioceramic were found to induce instantaneous inactivation Human herpesvirus 1 (HHV-1, also known as Herpes simplex virus or HSV-1). Si3N4 is non-oxide ceramic compound with strong antibacterial and antiviral properties that has been proven safe for human cells. HSV-1 double-stranded DNA infects variety host tissues through lytic latent cycle. Real-time reverse transcription (RT)-polymerase chain reaction (PCR)...
Invasive fungal infections caused by yeasts of the genus Candida carry high morbidity and cause systemic with mortality rate in both immunocompetent immunosuppressed patients. Resistance rates against antifungal drugs vary among species, most concerning specie being auris, which exhibits resistance to all major classes available drugs. The presently identification methods for species face a severe trade-off between testing speed accuracy. Here, we propose validate machine-learning approach...
Recent developments in Raman imaging at the microscopic scale were exploited here with specific purpose of locating spectral fingerprints individual membrane lipids and deoxynucleoside triphosphates during neuronal cell networking separation. After carefully screening spectra isolated lipid components, we located an situ mapped from phospholipids micrometric level comparison total distribution within single living cells. We concurrently examined silent zones emissions those peculiar ranges...
A large quantity of imported unripe green banana low quality is discarded, resulting in substantial waste cropped resources. novel powder (Banafine®) from Cavendish bananas was formulated by α-amylase and protease treatments followed yeast fermentation. Banafine® has a substantially improved taste as the bitter astringent eliminated. Pertinently, stimulated production IFN-ɣ TNF-α cultured macrophages phagocytosis. An vivo study murine model showed that stimulates cytokine splenocytes....
The multidrug-resistant Candida auris often defies treatments and presently represents a worldwide public health threat. Currently, the ergosterol-targeting Amphotericin B (AmB) DNA/RNA-synthesis inhibitor 5-flucytosine (5-FC) are two main drugs available for first-line defense against life-threatening infections. However, important aspects of their mechanisms action require further clarification, especially regarding metabolic reactions yeast cells. Here, we applied Raman spectroscopy...
The major 70 kDa heat shock protein (HSP70), which is scarcely expressed in unstressed rodent cells, was apparently induced by infection with herpes simplex virus (HSV). Infection HSV types 1 and 2 elevated HSP70 mRNA levels within 4 hr post-infection. synthesis accumulation increased HSV-infected cells. Irradiation of UV-light abolished the ability to induce mRNA. Inhibitors viral DNA did not affect induction infected Protein after necessary for induction.
Raman spectroscopy uncovered molecular scale markers of the viral structure SARS-CoV-2 Delta variant and related inactivation mechanisms at biological interface with silicon nitride (Si3N4) bioceramics. A comparison spectra collected on TY11-927 (lineage B.1.617.2; simply referred to as henceforth) those JPN/TY/WK-521 B.1.617.1; Kappa or Japanese isolate revealed occurrence key mutations spike receptor together profound structural differences in structure/symmetry sulfur-containing amino...