A5102769156- Supramolecular Self-Assembly in Materials
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Dendrimers and Hyperbranched Polymers
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Physiological and biochemical adaptations
- nanoparticles nucleation surface interactions
- biodegradable polymer synthesis and properties
- Proteins in Food Systems
- Surface Modification and Superhydrophobicity
- Icing and De-icing Technologies
- Microplastics and Plastic Pollution
- Lipid Membrane Structure and Behavior
- Protein Hydrolysis and Bioactive Peptides
- Silk-based biomaterials and applications
- Freezing and Crystallization Processes
- DNA and Nucleic Acid Chemistry
- Collagen: Extraction and Characterization
- Advanced Drug Delivery Systems
- Natural product bioactivities and synthesis
- Catalytic Processes in Materials Science
- Click Chemistry and Applications
- Biosensors and Analytical Detection
Kyoto Institute of Technology
2016-2025
Osaka University
2006-2013
King Mongkut's Institute of Technology Ladkrabang
2013
Protein Research Foundation
2011
Japan Science and Technology Agency
2006-2007
University of California, Berkeley
2004-2005
Point mutations and single base are the dominant found in KRAS gene. Systematic knockout of gene with these point is still challenging. In this study, we developed novel photo-cross-linking oligonucleotides (pU1-ODN pU2-ODN) that had a diazirine group at 5-position uridine derivatives. Photo-cross-linking studies wild-type mutated RNAs revealed pU2-ODN efficiently selectively reacts contain cytidine, guanosine, or residue frontal position pU2 nucleoside. These results suggest promising...
Aggregation of amyloid-β (Aβ) and tau plays a crucial role in the onset progression Alzheimer’s disease (AD). Therefore, inhibition Aβ aggregation may represent potential therapeutic target for AD. Herein, we designed synthesized both dual inhibitors based on structure curcumin developed novel derivative PE859. In this study, investigated inhibitory activity PE859 vitro effects cognitive dysfunction via vivo. inhibited protected cultured cells from Aβ-induced cytotoxicity. Furthermore,...
Peptide nanospheres with PEG brushes were prepared by the one-step polymerization of l-phenylalanine (Phe) N-carboxyanhydride dual initiators hydrophobic n-butylamine and hydrophilic NH2-monoterminated (NH2-PEG). We successfully peptide CH3O or COOH groups using CH3O- COOH-terminated as one initiators. The high-density brush conformation was confirmed 1H NMR measurements. FT-IR measurements suggested closely packed Phe polymer chains in core a β-strand conformation. results protein...
Ovalbumin (OVA), a non-inhibitory member of the serpin superfamily, forms fibrillar aggregates upon heat-induced denaturation. Recent studies suggested that OVA fibrils are generated by mechanism similar to amyloid fibril formation, which is distinct from polymerization mechanisms proposed for other serpins. In this study, we provide new insights into formation through identification amyloidogenic core regions using synthetic peptide fragments, site-directed mutagenesis, and limited...
The aim of this study was to elucidate the chemistry cellular degeneration in human neuroblastoma cells upon exposure outer-membrane vesicles (OMVs) produced by Porphyromonas gingivalis (Pg) oral bacteria monitoring their metabolomic evolution using situ Raman spectroscopy. Pg-OMVs are a key factor Alzheimer's disease (AD) pathogenesis, as they act efficient vectors for delivery toxins promoting neuronal damage. However, chemical mechanisms underlying direct impact on cell metabolites at...
We developed a centromere-associated protein E (CENP-E) inhibitor employing trans to cis photoisomerization with 405 nm visible light illumination and fast thermal relaxation.
Collagen, a major component of the extracellular matrix, contains Gly-Pro-Hyp repeats that form hydrogel. In this study, artificial collagen-mimetic materials were designed. Synthetic dendritic macromolecules fully modified with (Pro-Hyp-Gly)n and named dendrimers. A collagen-like triple helical structure was observed by circular dichroism spectrometry, an efficiency depended on peptide length. (Pro-Hyp-Gly)10-modified dendrimer exhibited most efficient helix formation. Thermal stability...
Abstract Collagen is the most abundant protein in mammals and widely used as a biomaterial for tissue engineering drug delivery. We previously reported that dendrimers linear polymers, modified with collagen model peptides (Pro‐Pro‐Gly) 5 , form collagen‐like triple‐helical structure; however, its triple helicity needs improvement. In this study, collagen‐mimic dendrimer longer peptides, 10 was synthesized named PPG10‐den. Circular dichroism analysis shows efficiency of helix formation...
Abstract Amyloid fibrils are supramolecular polymers with β‐sheet‐rich structures formed by polymerization of protein/peptide intermolecular interaction. have been miscast as toxic villains since they historically studied pathogens associated serious diseases, including Alzheimer's and Parkinson's disease. However, recent studies on their toxicity formation mechanism discovery functionality in nature correct the misconception strongly facilitate possible use β‐sheet‐forming peptides...
Surface-grafted peptide nanospheres consisting of hydrophobic poly(l-phenylalanine) with hydrophilic poly(ethylene glycol) (PEG) grafts were successfully prepared by the one-step polymerization l-phenylalanine N-carboxyanhydride dual initiators n-butylamine and NH2-monoterminated PEG (NH2−PEG). The monodispersed stably self-assembled during in a mixture water/dimethyl sulfoxide to create colloidal solution, but only aggregated water or organic solvents. When NH2−PEG was used as solitary...
Development of protein delivery systems is important for biomedical applications such as immunotherapy. Ovalbumin (OVA) a major component egg whites, and possible cause allergy. In this study, OVA was used model to develop system using guanidine-terminated dendrimers (Gdn-den) bearing an amyloid-promoting peptide derived from the helix B (hB) region (hB-Gdn-den). nanoparticles (NPs) were prepared by heat treatment OVA/hB-Gdn-den mixtures. The NP size surface charge controlled adjusting ratio...
Abstract Background Cervical cancer is a major global health concern with high prevalence in low- and middle-income countries. Natural products, particularly plant-derived compounds, have shown immense potential for developing anticancer drugs. In this study, we aimed to investigate the properties of pericarp seeds Sphaerocoryne affinis fruit on human cervical carcinoma cells (HeLa) isolate bioactive compound from active fraction. Methods We prepared solvent fractions ethanol extracts seed...
Reversibly photoswitchable chemical tools have aided in the development of novel approaches biomedical field. The visible region light should be ideal for biological application this approach because its low phototoxicity and deep penetration depth compared to ultraviolet light. Herein, we report a centromere-associated protein E (CENP-E) inhibitor, which is controllable with low-energy blue-green (around 500 nm) illumination. This tool enabled us control CENP-E-driven chromosome movements...
The amyloid fibril of a fragment the substrate binding site αA-crystallin (αAC(71–88)) exhibited chaperone-like activity by suppressing aggregation alcohol dehydrogenase (ADH) and luciferase. By contrast, cytotoxic β protein (Aβ(25–35)) facilitated same proteins. We have determined zeta potential measuring their electrophoretic mobility to study effects surface charge on modulation aggregation. αAC(71–88) possesses large negative value which is unaffected negatively charged ADH, indicating...
The development of ice-phobic, glass-substrate surfaces is important for many reasons such as poor visibility through the ice-covered windshields vehicles. present authors have developed new glass coated with a silane coupling agent and polypeptides whose amino-acid sequence identical to partial winter flounder antifreeze protein. We conducted experiments on freezing sessile water droplets surfaces, measured droplet temperature, contact angle, area surface roughness. results show that...
Recently, nanofibers (NFs) formed from antigenic peptides conjugated to β-sheet-forming have attracted much attention as a new generation of vaccines. However, studies describing how the hydrophilic-hydrophobic balance NF components affects cellular interactions NFs are limited. In this report, three different were prepared by self-assembly with model (SIINFEKL) ovalbumin and hydrophilic oligo-ethylene glycol (EG) differing chain lengths (6-, 12- 24-mer) investigate effect EG length...
The present study investigated the therapeutic effects of curcumin derivative 3-[(1E)-2-(1H-indol-6-yl)ethenyl]-5-[(1E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]ethenyl]-1H-pyrazole (GT863) in amyotrophic lateral sclerosis (ALS). inhibitory effect GT863 on superoxide dismutase 1 (SOD1) aggregation was evaluated cell-free assays. interfered with conformational changes SOD1 protein and later, oligomeric aggregation. Furthermore, its antioxidant, anti-inflammatory, neuroprotective were cultured...
The pentacyclic triterpenoid ursolic acid was previously shown to inhibit the intracellular trafficking of intercellular adhesion molecule-1 (ICAM-1) from endoplasmic reticulum (ER) Golgi apparatus. In present study, we further investigated biological activities three triterpenoids closely related on interleukin 1α-induced expression and ICAM-1. human lung adenocarcinoma A549 cells, asiatic acid, corosolic maslinic interfered with transport ICAM-1 cell surface. Endoglycosidase H-sensitive...