A5090236371- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- Cancer Genomics and Diagnostics
- Cancer-related Molecular Pathways
- Lung Cancer Research Studies
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Lymphoma Diagnosis and Treatment
- Cancer-related gene regulation
- RNA Research and Splicing
- Cancer Treatment and Pharmacology
- Gastric Cancer Management and Outcomes
- Chronic Lymphocytic Leukemia Research
- DNA Repair Mechanisms
- Liver Disease Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Neuroendocrine Tumor Research Advances
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
Chiba University Hospital
2024
Nagoya University
2014-2023
Tohoku University
1993-2023
Clinical Emergency Hospital Bucharest
2022
Aichi Cancer Center
2009-2021
Yokohama University of Pharmacy
2014-2020
Suntory Foundation for Life Sciences
2020
National Institute of Information and Communications Technology
2007-2019
National Taiwan University Hospital
2018
Taichung Veterans General Hospital
2018
Abstract In this study, we report for the first time reduced expression of let-7 microRNA in human lung cancers. Interestingly, 143 cancer cases that had undergone potentially curative resection could be classified into two major groups according to unsupervised hierarchical analysis, showing significantly shorter survival after with (P = 0.0003). Multivariate COX regression analysis showed prognostic impact independent disease stage (hazard ratio 2.17; P 0.009). addition, overexpression...
MicroRNAs (miRNAs) are small noncoding RNAs, thought to be involved in physiologic and developmental processes by negatively regulating expression of target genes. We have previously reported frequent down-regulation the let-7 miRNA family lung cancers and, present study, assessed alteration a panel 19 cancer cell lines. As result, we found for first time that miR-17-92 cluster, which comprises seven miRNAs resides intron 3 C13orf25 gene at 13q31.3, is markedly overexpressed cancers,...
Allele loss is a hallmark of chromosome regions harboring recessive oncogenes. Lung cancer frequently demonstrates heterozygosity on 17p. Recent evidence suggests that the p53 gene located 17p13 has many features such an antioncogene. The was mutated or inactivated in all types human lung cancer. genetic abnormalities include gross changes as homozygous deletions and abnormally sized messenger RNAs along with variety point small mutations, which map to open reading frame change amino acid...
Abstract Recently it has been reported that mutations in the tyrosine kinase domain of epidermal growth factor receptor(EGFR) gene occur a subset patients with lung cancer showing dramatic response to EGFR inhibitors. To gain further insights role carcinogenesis, we sequenced exons 18–21 using total RNA extracted from unselected 277 who underwent surgical resection and correlated results clinical pathologic features. were present 111 (40%). Fifty-two in-frame deletions around codons 746–750...
Purpose To evaluate the relationship between mutations of epidermal growth factor receptor (EGFR) gene and effectiveness gefitinib treatment in patients with recurrent lung cancer after pulmonary resection. Patients Methods We sequenced exons 18-21 EGFR using total RNA extracted from 59 who were treated for cancer. Gefitinib was evaluated by both imaging studies change serum carcinoembryonic antigen (CEA) levels. Results found 33 (56%). Of these mutations, 17 deletions around codons 746-750...
Emerging evidence suggests that microRNA, which are well‐conserved, abundant and small regulatory RNA, may be involved in the pathogenesis of human cancers. We recently reported expression let ‐7 was frequently reduced lung cancers, significantly associated with shorter patient survival. Two members double‐stranded RNA‐specific endonuclease family, Dicer Drosha , convert precursor forms microRNA into their mature using a stepwise process. In present study, we examined levels these genes 67...
Background: Vascular endothelial growth factor C (VEGF-C) stimulates tumor lymphangiogenesis (i.e., formation of lymphatic vessels) and metastasis to regional lymph nodes by interacting with VEGF receptor 3 (VEGFR-3). We sought determine whether inhibiting VEGFR-3 signaling, thus lymphangiogenesis, would inhibit metastasis. Methods: used the highly metastatic human lung cancer cell line NCI-H460-LNM35 (LNM35) its parental NCI-H460-N15 (N15) low capacity. inserted genes transfection...
Multiwalled carbon nanotubes (MWCNTs) have the potential for widespread applications in engineering and materials science. However, because of their needle-like shape high durability, concerns been raised that MWCNTs may induce asbestos-like pathogenicity. Although recent studies demonstrated various types reactivities, physicochemical features determine cytotoxicity carcinogenicity mesothelial cells remain unclear. Here, we showed deleterious effects nonfunctionalized on human were...
Histone H3 (H3) phosphorylation at Ser(10) occurs during mitosis in eukaryotes and was recently shown to play an important role chromosome condensation Tetrahymena. When producing monoclonal antibodies that recognize glial fibrillary acidic protein Thr(7), we obtained some cross-reacted with early mitotic chromosomes. They reacted 15-kDa phosphoprotein specifically cell lysate. With microsequencing, this proved be H3. Mutational analysis revealed they recognized Ser(28) phosphorylation. Then...
Abstract Lymphangiogenic growth factors vascular endothelial factor (VEGF)-C and VEGF-D have been shown to promote lymphatic metastasis by inducing tumor-associated lymphangiogenesis. In this study, we investigated how tumor cells gain access into vessels at what stage initiate metastasis. We show that VEGF-C produced induced extensive sprouting towards the as well dilation of draining vessels, suggesting an active role in A significant increase vessel occurred between 2 3 weeks after...
Autoimmune oophoritis that develops in A/J mice after neonatally thymectomy (NTx) was prevented by a single intraperitoneal injection of spleen cells or thymocytes from normal adult female mice. Prevention achieved when were given within 2 wk Tx. When obtained oophorectomized mice, four times more required for the prevention oophoritis, but those on day 7 birth had equivalent capacity to prevent The development NTx Thy-1+, Lyt-1+,23-, Ia-, Qa-1-, sensitive vitro irradiation with 400 rad,...
Purpose This study was conducted to gain insight into the relationship between expression profiles and underlying genetic changes, which are known be important for pathogenesis of lung cancers. Methods Expression 18,175 unique genes three major targets p53, epidermal growth factor receptor (EGFR), K-ras, were investigated in 149 patients with non–small-cell cancer, including 90 adenocarcinoma determine their relationships various clinicopathologic features Gene Ontology (GO) terms. Results...
Abstract MicroRNAs (miRNAs) are a distinct class of small noncoding RNAs that posttranscriptionally repress expression target genes through imperfect base pairing with the 3′ untranslated region. We previously reported amplification and overexpression miR-17-92 miRNA cluster at 13q31.3 in lung cancers, as well growth inhibition by treatment antisense oligonucleotides against miR-17-5p miR-20a, constituents miR-17-92, specifically miR-17-92–overexpressing cancer cell lines. Although these...
A serological study of immunoglobulin-forming cells the mouse, normal and malignant, shows that they lack all known surface differentiation antigens thymocyte-lymphocyte axis: TL, theta, Ly-A, Ly-B, MSLA. Two systems alloantigens are expressed on these cells, H-2 a new system named PC. The gene Pca (Plasma cell antigen) which specifies PC.1 alloantigen segregates as mendelian dominant not closely linked with H-2. This antigen is absent from thymocytes, leukemias, very probably thymus-derived...
Tissue-specific gene expression is mediated largely by transcription factors, and a master regulatory thus potential marker of cellular lineage. Using normal fetal through adult pulmonary tissues 64 consecutive lung adenocarcinomas, we examined the thyroid factor (TTF-1), which plays crucial role in function morphogenesis. TTF-1 was expressed consistently throughout life stages uniformly terminal respiratory unit, comprised peripheral airway cells small-sized bronchioles. Furthermore,...
Mice lacking the RelA (p65) subunit of NF-kappaB die between days 14 and 15 embryogenesis because massive liver destruction. Fibroblasts macrophages isolated from relA-/- embryos were found to be highly sensitive tumor necrosis factor (TNF) cytotoxicity, raising possibility that endogenous TNF is cause cell apoptosis. To test this idea, we generated mice both RelA. Embryogenesis proceeds normally in such mice, TNF/RelA double-deficient are viable have normal livers. Thus, RelA-mediated...