A5105658265- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Biofuel production and bioconversion
- Cellular Mechanics and Interactions
- Lignin and Wood Chemistry
- Angiogenesis and VEGF in Cancer
- Glycosylation and Glycoproteins Research
- Advanced Glycation End Products research
- S100 Proteins and Annexins
- Plant Gene Expression Analysis
- Connective tissue disorders research
- Nanoparticle-Based Drug Delivery
- Collagen: Extraction and Characterization
- Cell Image Analysis Techniques
- Bioenergy crop production and management
- Silk-based biomaterials and applications
- Fermentation and Sensory Analysis
- RNA Research and Splicing
- Proteoglycans and glycosaminoglycans research
- 3D Printing in Biomedical Research
- Electron and X-Ray Spectroscopy Techniques
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Nanoplatforms for cancer theranostics
- MicroRNA in disease regulation
Université de Reims Champagne-Ardenne
2016-2025
Fractionnation of AgroResources and Environment
2019
Biomatériaux et Bioingénierie
2019
Interface (United States)
2019
Centre Hospitalier Universitaire de Reims
2015
Inserm
2003-2008
Loss or dysfunction of the cystic fibrosis (CF) transmembrane conductance regulator (CFTR) leads to impairment airway mucus transport and chronic lung diseases resulting in progressive respiratory failure. Nicotinic acetylcholine receptors (nAChRs) bind nicotine nicotine-derived nitrosamines thus mediate many tobacco-related deleterious effects lung. Here we identify α7 nAChR as a key CFTR airways. The epithelium knockout mice is characterized by higher transepithelial potential difference,...
Abstract Lignocellulosic biomass is the only renewable carbon resource available in sufficient amount on Earth to go beyond fossil-based economy. Its transformation requires controlled breakdown of polymers into a set molecules make fuels, chemicals and materials. But network various inter-connected which are very difficult deconstruct optimally. In particular, saccharification potential lignocellulosic depends several complex chemical physical factors. For first time, an easily measurable...
Systemic sclerosis (SSc) is a systemic disease characterized by great clinical and immunological heterogeneity whose pathophysiology still being unraveled. Recently, innate immunity has been proposed to participate the pathogenesis of SSc. In this study, we investigated release neutrophil extracellular traps (NETs) according patient phenotype. Polymorphonuclear neutrophils (PMN) from 34 SSc patients 26 healthy controls were stimulated serum or subject. NETs visualized using epifluorescence...
Abstract Background The cell microenvironment, especially extracellular matrix proteins, plays an important role in tumor response to chemotherapeutic drugs. present study was designed investigate whether this microenvironment can influence the antimigratory effect of anthracycline drug, doxorubicin, when cells are grown a type I collagen, three-dimensional (3D) context which simulates natural microenvironment. Methods To purpose, we studied migratory parameters, integrin expression, and...
In addition to its critical role in lysosomes for catabolism of sialoglycoconjugates, NEU1 is expressed at the plasma membrane and regulates a myriad receptors by desialylation, playing key many pathophysiological processes. Here, we developed proteomic approach dedicated purification identification LC–MS/MS interaction partners human macrophages. Already known were identified as well several new candidates such class B scavenger receptor CD36. Interaction between CD36 was confirmed...
Abstract The elaboration, characterisation and efficiency of potential two‐photon‐excited photodynamic therapy (PDT) treatment new poly( d,l ‐lactide‐ co ‐glycolide) nanoparticles loaded with ruthenium(II) complexs are presented. materials based on the encapsulation Ru II complexes through an all‐biocompatible process. size is around 100 nm. internal concentration several orders magnitude higher than overall concentration, which leads to a more efficient targeted effect. therapeutic for PDT...
Background DNA extracellular traps (ETs), released by neutrophils (NETs) or eosinophils (EETs), play a pathogenic role in several autoimmune disorders. However, to date, NETs have never been investigated bullous pemphigoid (BP) with respect clinical and immunological activities, both at baseline time of relapse which characterized specific IL-17 IL-23 patterns. Objective: We sought assess whether ETs were associated BP as well the relative contribution axis cytokines NET induction. Methods:...
Chemoresistance remains one of the major obstacles to cancer treatment. The search for specific molecules that could improve treatment has become objectives biomedical research. Identifying new natural enhance chemotherapy or sensitization conventional therapies a key objective. Here, we evaluated effect Xanthohumol (XN) extracted from hop on SKMEL-28 melanoma cells and their vemurafenib (VEM) We measured XN cell viability apoptosis. also assessed membrane fluidity cholesterol levels....
During chronological skin aging, alterations in dermal structural proteins cause morphological modifications. Modifications are probably due to collagen fiber (type I collagen) rearrangement and reorientation with aging that have not been researched until now. FTIR microspectroscopy appears as an interesting method study protein structure under normal pathological conditions. Associated a polarizer, this vibrational technique permits us probe orientation within tissue sections, by computing...
A new Ir(iii) cyclometallated complex bearing a fluorenyl 5-substituted-1,10-phenanthroline ligand ([Ir(ppy)2()][PF6], ppy = 2-phenylpyridine) is presented which exhibits enhanced triplet oxygen sensing properties. The efficacy of this to act as photosensitiser for altering the morphology C6 Glioma cells that represent malignant nervous tumours has been evaluated. increased heavy metal effect and related spin-orbit coupling parameters on photophysical properties are evidenced by comparison...
Collagen IV is a major component of the dermo-epidermal junction (DEJ). To study expression collagen upon aging in DEJ and dermal fibroblasts isolated from same patients. A model senescent was developed order to identify biological compounds that might restore level IV. Skin fragments women (30 70 years old) were collected. Localisation studied by immunofluorescence. Fibroblast real-time PCR, ELISA, western blotting. Premature senescence simulated exposing subcytotoxic H2O2 concentrations....
Carcinogenesis occurs in elastin-rich tissues and leads to local inflammation elastolytic proteinase release. This contributes bioactive matrix fragment (Matrikine) accumulation like elastin degradation products (EDP) stimulating tumour cell invasive metastatic properties. We previously demonstrate that EDPs exert protumoural activities through Hsp90 secretion stabilised extracellular proteinases. EDP influence on cancer blebbing vesicle shedding were examined with a videomicroscope coupled...
The synthesis, optical properties and efficiency of new multifunctional nanoparticles as theranostic (fluorescence/MRI/PDT) agents are described. They based on a polysiloxane network surrounded by gadolinium(III) chelates ruthenium(II) complexes. size the is maintained under 5 nm in order to permit their efficient elimination from body. Their potential use agent (PDT/MRI) magnetic studied relaxometry (r1 = 9.21 mM−1 s−1 at 40 MHz; r2/r1 1.14) signal enhancement validated acquisition phantoms...
Emphysema is the major component of chronic obstructive pulmonary disease (COPD). During emphysema, elastin breakdown in lung tissue originates from release large amounts elastase by inflammatory cells. Elevated levels elastin-derived peptides (EP) reflect massive COPD patients. Only EP containing GXXPG conformational motif with a type VIII β-turn are receptor ligands inducing biological activities. In addition, COOH-terminal glycine residue seems prerequisite to activity. this study, we...
Lumican is a small leucine-rich proteoglycan (SLRP) being known as key regulator of collagen fibrillogenesis. However, little attention has been given so far in studying its influence on tumor-associated matrix architecture. Here, we investigate the role host lumican tumor organization well disease progression considering an immunocompetent model melanoma implanted Lum -/- vs. wild type syngeneic mice. Conjointly, impact response to matrix-targeted therapy was evaluated previously validated...
Staphylococcus aureus species is an important threat for hospital healthcare because of frequent colonization indwelling medical devices such as bone and joint prostheses through biofilm formations, leading to therapeutic failure. Furthermore, bacteria within are less sensitive the host immune system responses potential antibiotic treatments. We suggested that periprosthetic environment stressful bacteria, influencing development. To provide insights into S. properties three strains...
During aging, alterations of extracellular matrix proteins contribute to various pathological phenotypes. Among these alterations, type I collagen cross-linking and associated glycation products accumulation over time detrimentally affects its physico-chemical properties, leading tissue biomechanical stability. Here, different-age 3D matrices using non-destructive label-free biophotonic techniques were analysed highlight the impact aging on constructs, at macroscopic microscopic levels....