A5021804326- Ion channel regulation and function
- Ion Channels and Receptors
- Magnesium in Health and Disease
- Cardiac electrophysiology and arrhythmias
- Nicotinic Acetylcholine Receptors Study
- Bioactive Compounds and Antitumor Agents
- Plant Micronutrient Interactions and Effects
- Phytochemicals and Antioxidant Activities
- Ion Transport and Channel Regulation
- Estrogen and related hormone effects
- Neurobiology and Insect Physiology Research
- Protein Kinase Regulation and GTPase Signaling
- Neuroscience and Neural Engineering
- Chemical Reactions and Isotopes
- Trace Elements in Health
- Aluminum toxicity and tolerance in plants and animals
- Neuroscience and Neuropharmacology Research
- Pancreatic function and diabetes
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- Cellular Mechanics and Interactions
- Parathyroid Disorders and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Heat shock proteins research
- Carbohydrate Chemistry and Synthesis
Université de Picardie Jules Verne
2015-2024
Laboratoire de physiologie cellulaire et moléculaire
2010-2022
In-Q-Tel
2012
Consejo Superior de Investigaciones Científicas
2010
Inserm
1992-2004
Laboratoire de Physiologie Cellulaire
1994-2004
Université de Lille
1999-2001
Centre National de la Recherche Scientifique
1992-1995
Centre de Recherche en Biologie cellulaire de Montpellier
1991
Université de Tours
1987
Background: Transient Receptor Potential (TRP) channels are expressed in many solid tumors. However, their expression breast cancer remains largely unknown. Here, we investigated the profile of 13 TRP human ductal adenocarcinoma (hBDA) and performed a correlation between overexpression pathological parameters. Methods: The was determined by RT-PCR hBDA tissue, epithelial (hBCE) primary culture MCF-7 cell line. protein level evaluated immunohistochemistry tissue samples 59 patients. Results:...
Because transient receptor potential (TRP) channels have been implicated in tumor progression, we investigated the role of TRPM7 channel breast cancer cell proliferation. Under whole patch clamp, a Mg 2+ -inhibited cationic (MIC) current was observed MCF-7 cells. This characterized by an inward and strong outward rectifying that were both inhibited concentration-dependent manner presence intracellular or -ATP. The reduced La 3+ , sensitive to 2-aminoethoxydiphenyl borate (2-APB), spermine,...
Breast cancer (BC) is the leading in world terms of incidence and mortality women. However, mechanism by which BC develops remains largely unknown. The increase cytosolic free Ca(2+) can result different physiological changes including cell growth death. Orai isoforms are highly selective channels. In present study, we analyzed Orai3 expression normal cancerous breast tissue samples, its role MCF-7 MCF-10A mammary epithelial lines. We found that mRNAs was higher tissues cells than cells....
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive forms cancer with a tendency to invade surrounding healthy tissues, leading largely incurable disease. Despite many advances in modern medicine, there still lack early biomarkers as well efficient therapeutical strategies. The melastatin-related transient receptor potential 7 channel (TRPM7) nonselective cation that involved maintaining Ca(2+) and Mg(2+) homeostasis. It has been recently reported regulate cell...
Stormorken syndrome is a rare autosomal dominant disorder characterized by phenotype that includes miosis, thrombocytopenia/thrombocytopathy with bleeding time diathesis, intellectual disability, mild hypocalcemia, muscle fatigue, asplenia, and ichthyosis. Using targeted sequencing whole-exome sequencing, we identified the c.910C > T transition in STIM1 allele (p.R304W) only patients not their unaffected family members. encodes stromal interaction molecule 1 protein (STIM1), which finely...
Orai proteins are highly selective calcium channels playing an important role in entry. Orai3 overexpressed breast cancer (BC) tissues, and involved their proliferation, cell cycle progression survival. Herein, we sought to address the involvement of resistance chemotherapeutic drugs. Using high-throughput approaches, investigated major changes induced by overexpression, including downstream signaling mechanisms BC chemotherapy resistance. Resistance was dependent on external presence thus...
We have previously reported that the hEAG K(+) channels are responsible for potential membrane hyperpolarization induces human breast cancer cell progression into G1 phase of cycle. In present study, we evaluate role and functional expression intermediate-conductance Ca(2+)-activated channel, hIK1-like, in controlling cycle progression. Our results demonstrate hIK1 current density increased cells synchronized at end or S compared with those early phase. This paralleled enhancement mRNA...
Summary Isolated atrial or ventricular human cardio-myocytes were dissociated from the heart of patients undergoing either open surgery (CS) cardiac transplantation (CT). L-type Ca currents recorded on cardiomyocytes CS CT by whole-cell voltage clamp technique. Although electrophysiologic properties current similar in and myocytes patients, cells had larger capacitance lower density. Whereas isoprenaline (Iso) induced an increase amplitude both cells, a serotoninergic agonist (5-HT4)...
In various species, including humans, 5-hydroxytryptamine (5-HT) has been shown to exert positive chronotropic and inotropic cardiac effects through different types of receptors. The goal the present study was investigate regulation by 5-HT voltage-gated Ca2+ channels in human atrial myocytes characterize receptor involved. Cardiomyocytes isolated enzymatically mechanically were voltage-clamped using whole-cell configuration patch-clamp technique. Extracellular perfusion increased current...
The calcium-sensing receptor (CaR), is a G protein-dependent that responds to increments in extracellular Ca2+ ([Ca2+]o). We previously reported an increase [Ca2+]o induced release of intracellular calcium and entry via store operated channels (SOCs). also demonstrated MCF-7 cells express Transient Receptor Potential canonical 1 (TRPC1) channels. Herein, we investigated CaR signaling pathways examined the role TRPC1 CaR-induced cell proliferation, through signal-regulated Kinases & 2...
Abstract Background TRP channels have been shown to be involved in tumour generation and malignant growth. However, the expression of these breast cancer remains unclear. Here we studied function endogenous TRPC6 a cell line (MCF-7), human epithelial primary culture (hBCE) normal tissues. Methods Molecular (Western blot RT-PCR), immunohistochemical techniques were used investigate expression. To channel activity both MCF-7 cells hBCE electrophysiological technique (whole patch clamp...
Breast cancer (BC) has a poor prognosis due to its strong metastatic ability. Accumulating data present ether à go-go (hEag1) K(+) channels as relevant player in controlling cell cycle and proliferation of non-invasive BC cells. However, the role hEag1 invasive cells migration is still unknown. In this study, we studied both functional expression involvement highly MDA-MB-231 human We showed that mRNA proteins were expressed ductal carcinoma tissues lines. Functional activity was confirmed...
Abstract Background The calcium-permeable cation channel TRPM8 (melastatin-related transient receptor potential member 8) is over-expressed in several cancers. present study aimed at investigating the expression, function and regulation of channels by ER alpha (estrogen alpha) breast cancer. Methods RT-PCR, Western blot, immuno-histochemical, siRNA techniques were used to investigate its estrogen receptors, expression tissue. To activity MCF-7 cells, we whole cell patch clamp calcium imaging...
Epigenomic changes are an important feature of malignant tumors. How tumor aggressiveness is affected by DNA methylation specific loci largely unexplored. In genome-wide analyses, we identified the KCa 3.1 channel gene (KCNN4) promoter to be hypomethylated in aggressive non-small-cell lung carcinoma (NSCLC) cell line and patient samples. Accordingly, expression was increased more NSCLC cells. Both findings were strong predictors for poor prognosis adenocarcinoma. Increased associated with...
Collagen type 1 is among the tumor microenvironment (TM) factors, that regulates proliferation, survival, migration and invasion. Ion channels are key players in interactions between cells TM. Kv10.1 has been shown to play an essential role breast cancer cell proliferation by permitting Ca2+ influx notably via Orai1. Here, we show human (BC) growing, culture media completely devoid of serum seeded on collagen coating, exhibited less apoptotic rate a decrease Bax expression when compared...
// Malika Faouzi 1, 2 , Frederic Hague 1 Dirk Geerts 3 Anne-Sophie Ay Marie Potier-Cartereau 4 Ahmed Ahidouch Halima Ouadid-Ahidouch University of Picardie Jules Verne, UFR Sciences, EA4667 Laboratory Cell and Molecular Physiology, SFR CAP-SANTE (FED 4231), Amiens, France Queen's Center for Biomedical Research, The Medical Center, Honolulu, HI 96813, USA Department Pediatric Oncology/Hematology, Erasmus 3015 GE Rotterdam, Netherlands Inserm, UMR1069, Nutrition, Growth Cancer, François...
Abstract Background Ca 2+ is a ubiquitous messenger that has been shown to be responsible for controlling numerous cellular processes including cell growth and death. Whereas the involvement of IP 3 -induced signalling (IICS) in physiological activity types well documented, role IICS cancer cells still largely unknown. Our purpose was characterize control estrogen-dependent human breast epithelial line MCF-7 its potential regulation by 17β-estradiol (E 2 ). Results results show receptor (IP...