A5067982939- Autoimmune Bullous Skin Diseases
- Urticaria and Related Conditions
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Lipid metabolism and disorders
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Dermatology and Skin Diseases
- IL-33, ST2, and ILC Pathways
- Anorectal Disease Treatments and Outcomes
- Hidradenitis Suppurativa and Treatments
- Eosinophilic Esophagitis
- Kruppel-like factors research
- Proteoglycans and glycosaminoglycans research
- Axon Guidance and Neuronal Signaling
- Coronary Interventions and Diagnostics
- Diabetes and associated disorders
- Eosinophilic Disorders and Syndromes
- Tribology and Lubrication Engineering
- MicroRNA in disease regulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Systemic Sclerosis and Related Diseases
- interferon and immune responses
- Signaling Pathways in Disease
- Noise Effects and Management
- Lymphatic System and Diseases
Université de Reims Champagne-Ardenne
2014-2024
Collège de France
2003-2015
Biotherapy of Genetic Diseases, Inflammatory Disorders and Cancers
2015
Science for Life Laboratory
2012-2014
Uppsala University
2008-2014
Karolinska Institutet
2012
University of California, San Diego
2012
Inserm
2003-2011
Centre Interdisciplinaire de Recherche en Biologie
2011
Centre National de la Recherche Scientifique
2011
Angiopoietin-like 4 (ANGPTL4), a secreted protein of the angiopoietin-like family, is induced by hypoxia in both tumor and endothelial cells as well hypoxic perinecrotic areas numerous cancers. Here, we investigated whether ANGPTL4 might affect growth metastasis. Metastatic 3LL were therefore xenografted into control mice which was expressed using vivo DNA electrotransfer. Whereas primary tumors grew at similar rate groups, metastasized less efficiently to lungs that ANGPTL4. Fewer emboli...
Angiopoietin-like 4 (ANGPTL4) is a secreted protein that belongs to the angiopoietin family and involved in angiogenesis metabolism regulation. We previously reported induction of angptl4 by hypoxia endothelial cells human ischemic tissues from peripheral artery disease. here observed mouse model hindlimb ischemia mRNA upregulation vessels correlates with accumulation full-length tissues. then investigated its functions cells. In response hypoxia, endogenous ANGPTL4 accumulates...
Gradients of secreted signaling proteins guide growing blood vessels during both normal and pathological angiogenesis. However, the mechanisms by which endothelial cells integrate respond to graded distributions chemotactic factors are still poorly understood. We have in this study investigated cell migration response hill-shaped gradients vascular growth factor A (VEGFA) fibroblast 2 (FGF2) using a novel microfluidic chemotaxis chamber (MCC). Cell was scored at level individual time-lapse...
Increased permeability, predominantly controlled by endothelial junction stability, is an early event in the deterioration of vascular integrity ischemic disorders. Hemorrhage, edema, and inflammation are main features reperfusion injuries, as observed acute myocardial infarction (AMI). Thus, preservation fundamental heart disease. Angiopoietins pivotal modulators cell-cell junctions integrity. We hypothesized that hypoxic induction angiopoietin-like protein 4 (ANGPTL4) might modulate...
Abstract Bullous pemphigoid (BP) is an inflammatory autoimmune bullous disease involving cytokines and proteases in the process of blister formation. Recently, IL-17 IL-23 were evidenced lesional skin serum BP patients at time diagnosis, but their involvement outcome has still not been investigated yet. We then analysed levels during first months follow-up upon treatment. Compared with age- sex- matched controls, high observed baseline ( P < 0.01), while was not. However, some expressed...
By triggering an adaptive response to hypoxia which is a common feature of tumor microenvironments, endothelial cells contribute the onset angiogenic responses involved in growth. Therefore, identifying hypoxic markers represent challenge for better understanding angiogenesis and optimization anti‐angiogenic therapeutic strategy. Using representational difference analysis combined with microarray, we here report identification 133 hypoxia‐induced transcripts human microendothelial (HMEC‐1)....
Heparan sulfate proteoglycans regulate key steps of blood vessel formation. The present study was undertaken to investigate if there is a functional overlap between heparan and chondroitin during sprouting angiogenesis.Using cultures genetically engineered mouse embryonic stem cells, we show that angiogenic occurs also in the absence biosynthesis. Cells unable produce instead increase their production binds growth factors such as vascular endothelial factor A, transforming β,...
Abstract Hidradenitis suppurativa/acne inversa (HS) is a chronic, inflammatory, recurrent, debilitating skin disease of the hair follicle, associated with considerable tissue remodelling. Although abnormal cytokine expression was detected both in perilesional and uninvolved skin, up to now there no model allowing better understanding implicit inflammatory mechanisms HS. The aim this study investigate response HS by mean an ex vivo culture. To that purpose, nine biopsy specimens from patients...
Systemic sclerosis (SSc) is a systemic disease characterized by great clinical and immunological heterogeneity whose pathophysiology still being unraveled. Recently, innate immunity has been proposed to participate the pathogenesis of SSc. In this study, we investigated release neutrophil extracellular traps (NETs) according patient phenotype. Polymorphonuclear neutrophils (PMN) from 34 SSc patients 26 healthy controls were stimulated serum or subject. NETs visualized using epifluorescence...
Bullous Pemphigoid (BP) is an inflammatory rare autoimmune bullous dermatosis, which outcome cannot be predicted through clinical investigations. Eosinophils are the main immune infiltrated cells in BP. However, release of Major Basic Protein (MBP), Eosinophil Derived Neurotoxin (EDN), and Cationic (ECP) upon eosinophil activation has still not been evaluated with respect to BP development. MBP, EDN ECP were measured by ELISA serum (n = 61) blister fluid 20) patients at baseline, after 2...
Background DNA extracellular traps (ETs), released by neutrophils (NETs) or eosinophils (EETs), play a pathogenic role in several autoimmune disorders. However, to date, NETs have never been investigated bullous pemphigoid (BP) with respect clinical and immunological activities, both at baseline time of relapse which characterized specific IL-17 IL-23 patterns. Objective: We sought assess whether ETs were associated BP as well the relative contribution axis cytokines NET induction. Methods:...
Bullous Pemphigoid (BP) is a skin autoimmune blistering disease characterized by immune-mediated degradation of the dermo-epidermal junction and release large number inflammatory cytokines. Interleukin-1β (IL-1β) pleiotropic pro-inflammatory cytokine associated with inflammasome activation known to be pivotal in several auto-immune auto-inflammatory diseases. We sought clarify presence inflammasome-dependent IL-1β investigate its role BP. Skin biopsy specimens (n=13), serum (n=60), blister...
Bullous pemphigoid (BP) is the most common autoimmune bullous disease and typically affects elderly. Binding of specific autoantibodies to BP180/230 hemidesmosomal components induces an inflammatory response leading skin blister formation. Unusual manifestations BP include additional mucous membrane involvement, without pathophysiological knowledge associated formation these lesions. We here performed a prospective study on series consecutive patients with (n = 77) 18) mucosal involvements...
Chronic obstructive pulmonary disease (COPD) is a condition characterized by chronic airway inflammation and obstruction, primarily caused tobacco smoking. Although the involvement of immune cells in COPD pathogenesis well established, contribution innate lymphoid (ILCs) remains poorly understood. ILCs are type that participate tissue remodeling processes, but their specific role has not been fully elucidated. During COPD, breakdown elastin generates peptides elicit biological activities on...
Inflammation is largely implicated in bullous pemphigoid (BP), the most frequent skin auto-immune blistering disease. IL-17, essentially IL-17A/F, has been involved blister formation through regulation of protease production, and its specific serum profile within BP was related to disease outcome. However, relationships between IL-17 family ligands receptors are quite complex with six different isoforms, five receptors. We here aimed at clarifying contribution axis by characterizing not only...
Glucocorticoids are largely used in the treatment of inflammatory pathologies and/or hematological malignancies and regulate expression a variety genes involved inflammation or metastasis such as matrix metalloproteinases (MMP). Long-term exposure to glucocorticoids can result failure responsiveness, which is often associated with an unwanted gene expression. Epigenetic mechanisms modulated after development glucocorticoid resistance but how these take place must be further studied. The...
Bullous pemphigoid (BP) is a cutaneous autoimmune disease, characterized by an inflammatory cascade leading to blister formation. Although macrophages were shown participate in BP pathophysiology, their role the formation process still needs be investigated. We here addressed influence of serum and fluid (BF) from patients with on polarization status regards metalloproteinase-9 (MMP-9) expression. demonstrated that several markers related alternatively activated macrophage phenotype (M2)...
Abstract Optimizations are expected in the development of immunotherapy for treatment Triple‐negative breast cancer (TNBC). We studied expression galectin‐9 (Gal‐9) after irradiation and assessed differential impacts its targeting with or without radiotherapy. Tumor resections from TNBC patients who received neoadjuvant radiotherapy revealed higher levels Gal‐9 comparison to their baseline level, only non‐responder patients. was also found be increased tumor biopsies cell lines irradiation....
Abstract Background The lipodystrophic syndrome (LD) is a disorder resulting from selective damage of adipose tissue by antiretroviral drugs included in therapy controlling human-immunodeficiency-virus-1. In the cocktail nucleoside reverse transcriptase inhibitors (NRTI) contribute to development this syndrome. Cellular target NRTI was identified as mitochondrial polymerase-gamma and their toxicity described DNA (mtDNA) depletion cytopathy involved fat redistribution. No mechanisms offer...