A5007193297- Angiogenesis and VEGF in Cancer
- Microbial metabolism and enzyme function
- Receptor Mechanisms and Signaling
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Congenital heart defects research
- Nitric Oxide and Endothelin Effects
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Endoplasmic Reticulum Stress and Disease
- Hemodynamic Monitoring and Therapy
- Veterinary Pharmacology and Anesthesia
- Anesthesia and Pain Management
- Venous Thromboembolism Diagnosis and Management
- Intracranial Aneurysms: Treatment and Complications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Renin-Angiotensin System Studies
- Lipid metabolism and disorders
- Aortic aneurysm repair treatments
- Ultrasound in Clinical Applications
- Anesthesia and Sedative Agents
- Pancreatitis Pathology and Treatment
Centre Interdisciplinaire de Recherche en Biologie
2014-2024
Collège de France
2011-2024
Centre National de la Recherche Scientifique
2011-2024
Université de Nîmes
2014-2024
Université de Montpellier
2024
Inserm
2011-2024
Université Paris Sciences et Lettres
2019-2024
Centre Hospitalier Universitaire de Nîmes
2000-2024
Centre Henri Becquerel
2024
Université de Rouen Normandie
2024
The (pro)renin receptor [(P)RR] is a 35-kDa transmembrane protein that plays pivotal role in angiotensin tissue generation and nonproteolytic prorenin activation. We detected soluble form of (P)RR [s(P)RR; 28 kDa] the conditioned medium cultured cells. aims our study were to identify protease responsible for s(P)RR, site shedding, establish existence circulating s(P)RR plasma. identified furin as shedding endogenous based on following: LoVo colon carcinoma cells devoid active synthesize...
In skeletal muscle, new functions for vessels have recently emerged beyond oxygen and nutrient supply, through the interactions that vascular cells establish with muscle stem cells. Here, we demonstrate in human mouse endothelial (ECs) myogenic progenitor (MPCs) interacted together to couple myogenesis angiogenesis vitro vivo during regeneration. Kinetics of gene expression ECs MPCs sorted at different time points regeneration identified three effectors secreted by both MPCs. Apelin,...
Angiopoietin-like 4 (ANGPTL4) is a secreted protein that belongs to the angiopoietin family and involved in angiogenesis metabolism regulation. We previously reported induction of angptl4 by hypoxia endothelial cells human ischemic tissues from peripheral artery disease. here observed mouse model hindlimb ischemia mRNA upregulation vessels correlates with accumulation full-length tissues. then investigated its functions cells. In response hypoxia, endogenous ANGPTL4 accumulates...
Abstract Tissue engineering strategies based on implanting cellularized biomaterials are promising therapeutic approaches for the reconstruction of large tissue defects. A major hurdle reliable establishment such is lack rapid blood perfusion construct to provide oxygen and nutrients. Numerous sources mesenchymal stem cells (MSCs) displaying angiogenic potential have been characterized in past years, including adult dental pulp. Establishment efficient improving angiogenesis constructs...
The importance of PAR-1 in blood vessel development has been demonstrated knockout mice. As endothelial progenitor cells (EPCs) are involved postnatal vasculogenesis, we examined whether they express and stimulation by the peptide SFLLRN modulates their angiogenic properties.EPC expanded from human CD34+ cord expressed PAR-1. activation induced EPC proliferation a concentration-dependent manner far more potently than that umbilical vein cells. also enhanced actin reorganization, promoting...
Angiopoietin-like 4 (ANGPTL4) is involved in angiogenesis and lipid metabolism. It secreted by liver adipose tissues cleaved to generate circulating coiled-coil domain (CCD) fibrinogen-like (FLD) fragments. The full-length ANGPTL4 produced hypoxic endothelial cells interacts with the extracellular matrix (ECM). ECM-bound soluble forms of have antiangiogenic properties. We carried out a structure-function analysis investigate regulation bioactivity cells. found that recombinant CCD binds ECM,...
Transforming growth factor β1 (TGF-β1) is a pleiotropic involved in the regulation of extracellular matrix (ECM) synthesis and remodeling. In search for novel genes mediating action TGF-β1 on vascular ECM, we identified member lysyl oxidase family matrix-remodeling enzymes, oxidase-like 4 (LOXL4), as direct target aortic endothelial cells, dissected molecular mechanism its induction. Deletion mapping mutagenesis analysis LOXL4 promoter demonstrated absolute requirement distal enhancer...
Abstract Aims Recent trials provide conflicting results on the association between glucagon-like peptide 1 receptor agonists (GLP-1RA) and diabetic retinopathy (DR). The aim of AngioSafe type 2 diabetes (T2D) study was to determine role GLP-1RA in angiogenesis using clinical preclinical models. Methods We performed two studies humans. In 1, we investigated effect exposure from T2D diagnosis severity DR, as diagnosed with retinal imaging (fundus photography). 2, a randomized 4-week trial,...
Lysyl oxidases (LOXs) play a central role in extracellular matrix remodeling during development and tumor growth fibrosis through cross-linking of collagens elastin. We have limited knowledge the structure substrate specificity these secreted enzymes. LOXs share conserved C-terminal catalytic domain but differ their N-terminal region, which is composed 4 repeats scavenger receptor cysteine-rich (SRCR) domains LOX-like (LOXL) 2. investigated by X-ray scattering electron microscopy...
Although cell surface metalloendopeptidases degrade neuropeptides in the extracellular fluid to terminate signaling, function of peptidases endosomes is unclear. We report that isoforms endothelin-converting enzyme-1 (ECE-1a–d) are present early endosomes, where they and regulate post-endocytic sorting receptors. Calcitonin gene-related peptide (CGRP) co-internalizes with calcitonin receptor-like receptor (CLR), activity-modifying protein 1 (RAMP1), β-arrestin2, ECE-1 degrades CGRP. CGRP...
Abstract In vitro expansion of late endothelial progenitor cells (EPCs) might yield a cell therapy product useful for myocardial and leg ischaemia, but the influence EPC on angiogenic properties these is unknown. present study, we investigated effect in vascular growth factor (VEGF) receptor expression. EPCs were obtained from CD34 + cord blood expanded up to 5 weeks. Real‐time quantitative reverse‐transcription polymerase chain reaction (RT‐PCR) showed that VEGFR2 expression, contrary...
Neuropeptide signaling requires the presence of G protein-coupled receptors (GPCRs) at cell surface. Activated GPCRs interact with beta-arrestins, which mediate receptor desensitization, endocytosis, and mitogenic signaling, peptide-receptor-arrestin complex is sequestered into endosomes. Although dissociation beta-arrestins required for recycling resensitization, critical event that initiates this process unknown. Here we report agonist availability in endosomes, controlled by membrane...
Abstract Neural crest cells (NCCs) are a population of multipotent that migrate extensively during vertebrate development. Alterations to neural ontogenesis cause several diseases, including cancers and congenital defects, such as Hirschprung disease, which results from incomplete colonization the colon by enteric NCCs (ENCCs). We investigated influence stiffness structure environment on ENCC migration in vitro gastrointestinal tract chicken mouse embryos. showed using tensile stretching...
Clear cell renal carcinoma (ccRCC) is largely driven by Von Hippel Lindau (VHL) protein deficiency, promoting epithelial-mesenchymal transition, invasion, and hypervascularization, mediated vascular endothelial growth factor (VEGF) signaling resulting in a structurally abnormal capillary network, which remains insufficiently defined. Previous studies correlating patient outcome with microvascular density yielded diverse results, underscoring the limitations of conventional parameters to...
Among the members of prohormone convertase (PC) family, PC2 has a unique maturation pattern: it is retained in ER for comparatively long time and its propeptide cleaved TGN/ secretory granules rather than ER. It also by association with neuroendocrine protein 7B2. This interaction results facilitation proPC2 production activatable from CHO cells. In present study, we have investigated mechanism this interaction. ProPC2 binds 7B2 ER, but exits compartment much more slowly We found that was...
Endothelin-converting enzyme (ECE) is a membrane metalloprotease that generates endothelin from its direct precursor big endothelin. Four isoforms of ECE-1 are produced single gene through the use alternate promoters. These share same extracellular catalytic domain and contain unique cytosolic tails, which results in their specific subcellular targeting. We investigated distribution transfected AtT-20 neuroendocrine cells. Whereas ECE-1a 1c were present at plasma membrane, ECE-1b ECE-1d...
Sprouting angiogenesis is stimulated by vascular endothelial growth factor (VEGF165) that localized in the extracellular matrix (ECM) and binds to heparan sulfate (HS)-bearing proteins known as proteoglycans (HSPGs). VEGF165 presentation HSPGs enhances VEGF receptor-2 (VEGFR2) signaling. We investigated effect of TG2, which HSPGs, on interaction between HS angiogenesis. Mice with tg2 deficiency showed transiently enhanced retina vessel formation increased vascularization VEGF165-containing...