A5026220425- Collagen: Extraction and Characterization
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Bone and Dental Protein Studies
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Graphene and Nanomaterials Applications
- Cell Adhesion Molecules Research
- Nanoparticle-Based Drug Delivery
- Diatoms and Algae Research
- Surgical Sutures and Adhesives
- Spine and Intervertebral Disc Pathology
- biodegradable polymer synthesis and properties
- Proteoglycans and glycosaminoglycans research
- Supramolecular Self-Assembly in Materials
- Tendon Structure and Treatment
- Osteoarthritis Treatment and Mechanisms
- Magnetism in coordination complexes
- Antimicrobial agents and applications
- Polymer Surface Interaction Studies
- Biocrusts and Microbial Ecology
Chimie de la Matière Condensée de Paris
2016-2025
Sorbonne Université
2016-2025
Centre National de la Recherche Scientifique
2013-2025
Laboratoire de Physique Théorique de la Matière Condensée
2014-2025
Sorbonne Paris Cité
2023
Centre des Matériaux
2015-2023
Université Paris Cité
1996-2022
Institut de Psychiatrie et Neurosciences de Paris
2022
SPPIN - Saints-Pères Paris Institute for Neurosciences
2022
Laboratoire Analyse et Modélisation pour la Biologie et l'Environnement
2021
Silica-collagen type I nanocomposite hydrogels are evaluated as medicated dressings to prevent infection in chronic wounds. Two antibiotics, gentamicin and rifamycin, encapsulated a single step within plain silica nanoparticles. Their antimicrobial efficiency against Pseudomonas aeruginosa Staphylococcus aureus is assessed. Gentamycin-loaded 500 nm particles can be immobilized at high dose concentrated collagen without modifying their fibrillar structure or impacting on rheological behavior...
Diabetic foot ulcers (DFUs) are characterized by a chronic inflammation state which prevents cutaneous wound healing, and DFUs eventually lead to infection leg amputation. Macrophages located in locked an pro-inflammatory phenotype. In this study, the effect of hyperglycemia hypoxia on macrophage phenotype was analyzed. For purpose, microarray performed study gene expression profile macrophages cultivated high glucose concentration. Hyperglycemia upregulated cytokines such as TNF-α, IL-1,...
Biofabrication is providing scientists and clinicians the ability to produce engineered tissues with desired shapes gradients of composition biological cues. Typical resolutions achieved extrusion-based bioprinting are at macroscopic level. However, for capturing fibrillar nature extracellular matrix (ECM), it necessary arrange ECM components smaller scales, down micron molecular Herein, we introduce a bioink containing tyramine derivative hyaluronan (HA; henceforth known as THA) collagen...
Associating collagen with biodegradable hydrophobic polyesters constitutes a promising method for the design of medicated biomaterials. Current collagen-polyester composite hydrogels consisting pre-formed polymeric particles encapsulated within low concentrated hydrogel suffer from poor physical properties and drug loading. Herein, an amphiphilic platform associating dense up to 50 wt% different hydrophobicity chain length is developed. An original fabrication disclosed based on in situ...
Hybrid nanoparticles have great potential for biotechnological and biomedical applications. It was recently proposed that biopolymer/silica nanomaterials could be easily obtained by adapting traditional routes used in pharmaceutical science to design polymer nanoparticles. In this paper, we demonstrate core–shell gelatine/silica can using a nano-emulsion route, taking advantage of the thermal gelation properties biopolymer its strong "biomimetic" affinity silicates. The formation silica...
Cutaneous chronic wounds are characterized by an impaired wound healing which may lead to infection and amputation. When current treatments not effective enough, the application of dressings is required. To date, no ideal biomaterial available. In this study, highly dense collagen matrices have been evaluated as novel medicated for treatment wounds. For purpose, structure, mechanical properties, swelling ability in vivo stability concentrated from 5 40 mg mL(-1) were tested. The matrix...
The craniofacial area is prone to trauma or pathologies often resulting in large bone damages. One potential treatment option the grafting of a tissue-engineered construct seeded with adult mesenchymal stem cells (MSCs). dental pulp appears as relevant source MSCs, display strong osteogenic properties and are efficient at formation repair. Fibroblast growth factor-2 (FGF-2) and/or hypoxia primings were shown boost angiogenesis from human exfoliated deciduous teeth (SHED). Based on these...
Several methods have already been developed for the design of (bio)polymer micro- and nanoparticles used in pharmaceutical science. In this paper, we demonstrate that some these approaches can be easily adapted hybrid nanocapsules. A microgel route synthesis poly-L-lysine/alginate microparticles, are further coated with silica, leading to micron sized core shell composites. Alternatively, a spray-drying technique is one-pot silica/poly-L-lysine/alginate nanocomposites exhibit homogeneous...
Silica–collagen scaffolds are synthesized by the simultaneous polymerization of aqueous silicates and self-assembly protein triple helices in presence living human dermal fibroblasts.
Apligraf®, a skin substitute currently used in chronic wound treatment, acts as source of macromolecules and cytokines to promote healing. Normal collagen hydrogel (NCH), obtained from at low concentration (0.66 mg/ml), is the base dermal layer. Apligraf has several drawbacks, such poor persistence fibroblasts within normal hydrogel. In present study we have evaluated concentrated hydrogels 5 mg/ml (CCH5s) substitutes for treatment wounds. The effect raised on stability, cell growth,...
Since their first description nearly 20 years ago, dense collagen hydrogels obtained by plastic compression have become popular scaffolds in tissue engineering. In particular, when seeded with dental pulp stem cells, they demonstrated a great vivo potential cranial bone repair. Here, we investigated how physico-chemical and cell-seeding conditions could influence the formation vitro mineralization of these cellularized scaffolds. A qualitative assessment that gel stability before after was...
Hybrid and nanocomposite silica-collagen materials derived from concentrated collagen hydrogels were evaluated in vitro vivo to establish their potentialities for biological dressings. Silicification significantly improved the mechanical thermal stability of network within hybrid systems. Nanocomposites found favor metabolic activity immobilized human dermal fibroblasts while decreasing hydrogel contraction. Cell adhesion experiments suggested that cell behavior was dictated by properties...
Anti-inflammatory cytokine IL-10 produced and released from collagen–silica nanocomposites is capable of modulating macrophage phenotype in a 3D inflammation model.
Local delivery of biomolecules from hydrogels is highly challenging because their rapid diffusion and degradation. Gene therapy represents an alternative that allows for the prolonged production proteins by transfected cells. In this study, we have developed nanocomposites consisting DNA-polyethylenimine-silica nanoparticle complexes coencapsulated with fibroblasts within collagen hydrogels. Through modulation particle size polyethylenimine molecular weight, it was possible to achieve...
The photo-induced synthesis of chitosan–PEG hydrogels with tremendous antibacterial and anti-adhesive properties even after 6 months’ storage.