A5074849896- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pulmonary Hypertension Research and Treatments
- Bone health and treatments
- Bone and Dental Protein Studies
- dental development and anomalies
- Parathyroid Disorders and Treatments
- Mesenchymal stem cell research
- Systemic Sclerosis and Related Diseases
- Pleistocene-Era Hominins and Archaeology
- Tissue Engineering and Regenerative Medicine
- Forensic Anthropology and Bioarchaeology Studies
- Bone Metabolism and Diseases
- Endodontics and Root Canal Treatments
- Periodontal Regeneration and Treatments
- Wildlife Ecology and Conservation
- Oral and gingival health research
- Paleopathology and ancient diseases
- Chronic Myeloid Leukemia Treatments
- Inflammasome and immune disorders
- Wnt/β-catenin signaling in development and cancer
- Congenital Ear and Nasal Anomalies
- Cell death mechanisms and regulation
- Dental materials and restorations
- Alkaline Phosphatase Research Studies
- Neonatal Respiratory Health Research
Université Paris Cité
2015-2024
Sorbonne Paris Cité
2015-2024
Laboratory Orofacial Pathologies, Imaging and Biotherapies
2016-2023
Délégation Paris 5
2015-2018
Autorité de Sûreté Nucléaire
2018
Descartes (Belgium)
2017
Abstract Therapies using mesenchymal stem cell (MSC) seeded scaffolds may be applicable to various fields of regenerative medicine, including craniomaxillofacial surgery. Plastic compression collagen with MSC has been shown enhance the osteogenic differentiation as it increases fibrillary density. The aim present study was evaluate effects dense gel dental pulp cells (DPSC) on bone regeneration in a rat critical-size calvarial defect model. Two symmetrical full-thickness defects were created...
Abstract Tissue engineering strategies based on implanting cellularized biomaterials are promising therapeutic approaches for the reconstruction of large tissue defects. A major hurdle reliable establishment such is lack rapid blood perfusion construct to provide oxygen and nutrients. Numerous sources mesenchymal stem cells (MSCs) displaying angiogenic potential have been characterized in past years, including adult dental pulp. Establishment efficient improving angiogenesis constructs...
Abstract Wnt proteins are lipid-modified, short-range signals that control stem cell self-renewal and tissue regeneration. We identified a population of responsive cells in the pulp cavity, characterized their function, then created injury. The repair response was evaluated over time using molecular, cellular, quantitative assays. tested how healing impacted by wound environments which signaling amplified. found Wnt-amplified environment associated with superior healing. Although death still...
Objective To evaluate the antifibrotic effects of pan-peroxisome proliferator-activated receptor (PPAR) agonist IVA337 in preclinical mouse models pulmonary fibrosis and related hypertension (PH). Methods has been evaluated model bleomycin-induced Fra-2 transgenic mice, this latter being characterised by non-specific interstitial pneumonia severe vascular remodelling arteries leading to PH. Mice received two doses (30 mg/kg or 100 mg/kg) vehicle administered daily oral gavage up 4 weeks....
The craniofacial area is prone to trauma or pathologies often resulting in large bone damages. One potential treatment option the grafting of a tissue-engineered construct seeded with adult mesenchymal stem cells (MSCs). dental pulp appears as relevant source MSCs, display strong osteogenic properties and are efficient at formation repair. Fibroblast growth factor-2 (FGF-2) and/or hypoxia primings were shown boost angiogenesis from human exfoliated deciduous teeth (SHED). Based on these...
Significance Fibrosis is a leading cause of death in industrialized countries. Until now, there has been no effective therapy to prevent or counteract the fibrotic process. This article describes effect blockade late costimulatory molecule inflammation-driven skin, lung, and vessel fibrosis induce regression established dermal vivo complementary murine models systemic sclerosis, prototypic autoimmune disease. also reveals an unexpected role this protein as biomarker worsening that might help...
While calcium oxalate monohydrate (whewellite: CaC2O4·H2O) kidney stones are related to hyperoxaluria, dihydrate (weddellite: CaC2O4·(2 + x)H2O) ones mainly associated with hypercalciuria. Crystalline conversion from weddellite whewellite introduces a contradiction between Fourier transform infrared (FTIR) spectra which indicate the presence of and fact that bipyramid crystallites, morphology specific weddellite, can be observed. It constitutes thus major problem for clinicians as...
We aimed to investigate the efficacy of abatacept in preclinical mouse models digestive involvement, pulmonary fibrosis, and related hypertension (PH), mimicking internal organ involvement systemic sclerosis (SSc).Abatacept has been evaluated chronic graft-versus-host disease (cGvHD) model (abatacept 1 mg/mL for 6 weeks), characterized by liver intestinal fibrosis Fra-2 (1 or 10 4 interstitial lung (ILD) vascular remodeling leading PH.In cGvHD model, significantly decreased transaminase...
Abstract Background Genetically engineered animals are essential for gaining a proper understanding of the disease mechanisms cystic fibrosis (CF). The rat is relevant laboratory model CF because its zootechnical capacity, size, and airway characteristics, including presence submucosal glands. Methods We describe generation (F508del) homozygous p.Phe508del mutation in transmembrane conductance regulator ( Cftr ) gene. This was compared to new −/− rats (CFTR KO). Target organs were examined...
Nowadays, the preservation of dental pulp vitality is an integral part our daily therapies. The success these treatments depends on clinical situation as well biomaterials used. Mineral Trioxide aggregate and BiodentineTM are commonly used capping materials. One objective vital therapy repair/regeneration pulp. In addition to initial inflammatory status pulp, nature quality new mineralized tissue obtained after directly influence treatment. order characterize reparative dentin, in current...
Abstract Overexpression of the nucleotide‐binding leucine‐rich repeat protein 3 (NLRP3) inflammasome in chronic auto‐immune diseases leads to skeletal anomalies, with severe osteopenia due activation osteoclasts. Reproducing this phenotype Nlrp3 knock‐in mice has provided insights into role NLRP3 bone metabolism. We studied physiological development using a complete knock‐out mouse model. found impaired −/− mice, resulting shorter stature than that +/+ mice. These growth defects were...
In humans, the SOST gene encodes sclerostin, an inhibitor of bone growth and remodeling, which also negatively regulates repair process. Sclerostin has been implicated in tooth formation, but its potential role pulp healing remains unknown. The aim this study was to explore sclerostin reparative dentinogenesis using Sost knockout mice (Sost–/–). pulps first maxillary molars were mechanically exposed 3-mo-old Sost–/– wild-type (WT) (n = 14 per group), capped with mineral trioxide aggregate...
The NLRP3 inflammasome is overexpressed in gingiva of periodontitis patients but its role remains unclear. In our study, we use a mouse model ligature, impregnated or not with Porphyromonas gingivalis , WT KO mice. After 28 days induction, ligature alone provoked exacerbated periodontal destruction mice, compared to an increase activated osteoclasts. No difference was observed at 14 days, suggesting that involved regulatory pathways limit periodontitis. contrast, the presence P. this...
Objective To elucidate the role of gene candidates involved in pulmonary hypertension ( PH ) associated with systemic sclerosis SS c). Methods Gene were identified through microarray experiments performed on Affymetrix GeneChip Human Exon 1.0 ST arrays endothelial progenitor cell EPC )–derived cells EC s) obtained from patients c‐associated , c without and healthy control subjects. Expression was assessed by quantitative sandwich enzyme‐linked immunosorbent assay serum, immunohistochemistry...
Dental pulp is a dynamic tissue able to heal after injury under moderate inflammatory conditions. Our study aimed evaluate repair conditions in rats. For this purpose, we developed rat model of controlled pulpitis followed by pulpotomy with tricalcium silicate-based cement. Fifty-four cavities were prepared on the occlusal face maxillary upper first molar 27 eight-week-old male E. coli lipopolysaccharides at 10 mg/mL or phosphate-buffered saline PBS was injected injury. Non-inflamed molars...
The Leucine Rich Amelogenin Peptide (LRAP) is a product of alternative splicing the amelogenin gene. As full length amelogenin, LRAP has been shown, in precipitation experiments, to regulate hydroxyapatite (HAP) crystal formation depending on its phosphorylation status. However, very few studies have questioned impact status enamel mineralization biological models. Therefore, we analyzed effect phosphorylated (+P) or non-phosphorylated (-P) ameloblast-like cell lines and ex vivo cultures...
X-linked hypophosphatemia (XLH) is the most common form of genetic rickets. Mainly diagnosed during childhood because growth retardation and deformities lower limbs, disease affects adults with early enthesopathies joint structural damage that significantly alter patient quality life. The conventional treatment, based on phosphorus supplementation active vitamin D analogs, commonly administered from to end growth; unfortunately, it does not allow complete recovery skeletal damage. Despite...
Abstract Sarcopenia involves a progressive loss of skeletal muscle force, quality and mass during ageing, which results in increased inability death; however, no cure has been established thus far. Growth differentiation factor 5 (GDF5) described to modulate maintenance various contexts. For our proof concept, we overexpressed GDF5 by AAV vector injection tibialis anterior adult aged (20 months) mice performed molecular functional analysis muscle. We analysed human vastus lateralis biopsies...
Abstract Bone diseases are increasing with aging populations and it is important to identify clues develop innovative treatments. Vasn , which encodes vasorin (Vasn), a transmembrane protein involved in the pathophysiology of several organs, expressed during development intramembranous endochondral ossification zones. Here, we studied impact deletion on osteoblast osteoclast dialog through cell Coculture model. In addition, explored bone phenotype KO mice, either constitutive or...