A5074713980- Spectroscopy Techniques in Biomedical and Chemical Research
- Dental materials and restorations
- Spectroscopy and Chemometric Analyses
- Photoacoustic and Ultrasonic Imaging
- Dental Implant Techniques and Outcomes
- Dental Radiography and Imaging
- Diamond and Carbon-based Materials Research
- Endodontics and Root Canal Treatments
- Periodontal Regeneration and Treatments
- Laser Applications in Dentistry and Medicine
- Child Nutrition and Feeding Issues
- Mesenchymal stem cell research
- Protein Interaction Studies and Fluorescence Analysis
- 3D Printing in Biomedical Research
- Oral microbiology and periodontitis research
- Bone Tissue Engineering Materials
- Analytical Chemistry and Sensors
- Peroxisome Proliferator-Activated Receptors
- Ultrasound Imaging and Elastography
- Autism Spectrum Disorder Research
- Photodynamic Therapy Research Studies
- Dental Erosion and Treatment
- Bee Products Chemical Analysis
- Cancer Cells and Metastasis
- Nanoparticles: synthesis and applications
Laboratoire des Sciences de l'Ingénieur, de l'Informatique et de l'Imagerie
2023-2025
Inserm
2025
Université de Strasbourg
2025
Centre National de la Recherche Scientifique
2025
Laboratoire Bioingénierie et Nanosciences
2011-2024
Université de Montpellier
2013-2023
Lyon College
2022
Université Claude Bernard Lyon 1
2022
Leibniz Institute of Photonic Technology
2022
Géosciences Montpellier
2020
The variable configuration of Raman spectroscopic platforms is one the major obstacles in establishing spectroscopy as a valuable physicochemical method within real-world scenarios such clinical diagnostics. For real world applications like diagnostic classification, models should ideally be usable to predict data from different setups. Whether it done by training rugged model with many setups or primary-replica strategy where are developed on 'primary' setup and test generated 'replicate'...
Nanotechnology applied to cancer treatment is a growing area of research in nanomedicine with magnetic nanoparticle-mediated anti-cancer drug delivery systems offering least possible side effects. To that end, both structural and chemical properties commercial cobalt metal nanoparticles were studied using label-free confocal Raman spectroscopy.Crystal structure morphology by XRD TEM. Magnetic SQUID PPMS. Confocal microscopy has high spatial resolution compositional sensitivity. It,...
Dental histology is a field that has been studied since the early 19th century. Most of techniques used have based on white-light microscopy or histological staining, more recently immunohistochemical staining. With advent lasers coupled to confocal microscopy, Raman spectra can be measured in large numbers create detailed chemical atlas human tooth, offering new insights into its composition and structure. A total twenty teeth, with 5 teeth from each type premolar, molar, incisor canine...
Nowadays, the preservation of dental pulp vitality is an integral part our daily therapies. The success these treatments depends on clinical situation as well biomaterials used. Mineral Trioxide aggregate and BiodentineTM are commonly used capping materials. One objective vital therapy repair/regeneration pulp. In addition to initial inflammatory status pulp, nature quality new mineralized tissue obtained after directly influence treatment. order characterize reparative dentin, in current...
Confocal Raman microscopy, a non-invasive, label-free, and high spatial resolution imaging technique is employed to trace the anticancer drug paclitaxel in living Michigan Cancer Foundation-7 (MCF-7) cells. The images were treated by K-mean cluster analysis detect Distribution of cells verified calculating correlation coefficient between reference spectrum whole image spectra. A time dependent gradual diffusion all over cell observed suggesting complementary picture pharmaceutical action...
Understanding stem cell behavior as a delivery tool in cancer therapy is essential for evaluating their future clinical potential. Previous in-vivo studies proved the use of mesenchymal cells (MSCs) local commonest anticancer drug, paclitaxel (PTX). Dental pulp relatively abundant noninvasive source MSCs. We assess dental (DPSCs), first time, drug carriers. Confocal Raman microscopy unique to trace and viability without labeling. Drug uptake apoptosis are identified through confocal...
Confocal Raman microscopy is a noninvasive, label-free imaging technique used to study apoptosis of live MCF-7 cells. The images are based on spectra cells components, and their monitored through diffusion cytochrome c in cytoplasm. K-mean clustering identify mitochondria cells, correlation analysis provides the distribution inside Our results demonstrate that incubation for 3 h with 10 μM paclitaxel does not induce On contrary, 30 min at higher concentration (100 μM) induces gradual release...
The separation zone between enamel and dentin [dentin–enamel junction (DEJ)] with different properties in biomechanical composition has an important role preventing crack propagation from to dentin. understanding of the chemical structure (inorganic organic components), physical properties, human DEJ could benefit biomimetic materials dentistry. Spatial distribution calcium phosphate crystallinity collagen crosslinks near were studied using confocal Raman microscopy calculated by methods. To...
Abstract Dental enamel is the most mineralized tissue in human body and has a very complex organization. The aim of present study was to evaluate chemical composition orientation crystals cross striation using confocal Raman microscope. Slices teeth were prepared scanned Cross spacing calculated according variation PO 4 3− peak intensity. measurements showed characteristic length between two striations 2.70 ( SD 0.43) μm for permanent 1.75 0.37) deciduous teeth. Ratios OH peaks, 0 90 degrees...
Aim: Calcium silicate cements are widely used in endodontics. Novel fast-setting calcium cement with fluoride (Protooth) has been developed for potential applications teeth crowns including cavity lining and cementation. Objective: To evaluate the surface apatite-forming ability of Protooth compositions as a function content immersion time phosphate-buffered saline (PBS). Material methods: Three were tested: (3.5% 10% radiocontrast), ultrafast 20% high (15% 25% radiocontrast). Powders...
White lesions represent an early phase of caries formation. 20 human sound premolars were subjected to pH cycling procedure induce subsurface (SLs) in vitro. In addition, 2 teeth with naturally developed white spot (WSLs) used as references. All specimens characterized by confocal Raman microscopy being for the first time examining & and providing a high resolution chemical morphological map based on phosphate peak intensity alterations at 960 cm
In recent years, fluorescent nanodiamond (fND) particles containing nitrogen-vacancy (NV) centers gained recognition as an attractive probe for nanoscale cellular imaging and quantum sensing. For these applications, precise localization of fNDs inside a living cell is essential. Here we propose such method by simultaneous detection the signal from NV spectroscopic Raman cells to visualize nucleus cells. However, show that commonly used fingerprint region not suitable organelle in this case....
Further development of biomaterials is expected as advanced therapeutic products must be compliant to good manufacturing practice regulations. A spraying method for building-up polyelectrolyte films followed by the deposition dental pulp cells presented. Physical treatments UV irradiation and a drying/wetting process are applied system. Structural changes elasticity modifications obtained coatings revealed atomic force microscopy Raman spectroscopy. This procedure results in thicker, rougher...
Confocal Raman microscopy, a noninvasive, label‐free, and high‐spatial resolution imaging technique, in combination with K ‐mean cluster analysis correlation coefficient map, was employed to trace titanium dioxide (TiO 2 ) nanoparticles living MCF‐7 TERT cells. The penetration of TiO into cells revealed gradual time‐dependent diffusion over the entire cell. Cell apoptosis monitored by tracing cytochrome c cytoplasm. A comparison mitochondrial clustering indicated that inside mitochondria for...
SARS-CoV-2 infection remains spread worldwide and requires a better understanding of virus-host interactions. Here, we analyzed biochemical modifications due to in cells by confocal Raman microscopy. Obtained results were compared with the another RNA virus, measles virus. Our have demonstrated virus-specific molecular signature, reflecting intracellular modification during each infection. Advanced data analysis has been used distinguish non-infected versus infected for two viruses. Further,...
Regenerative medicine brings promising applications for mesenchymal stem cells, such as dental pulp cells (DPSCs). Confocal Raman microscopy, a noninvasive technique, is used to study osteogenic differentiation of DPSCs. Integrated intensities in the 2800 3000 cm⁻¹ region (C-H stretching) and 960 peak (ν₁ PO₄³⁻) were collected (to image phosphate, respectively), ratio two peaks 1660 over 1690 (amide I bands) measure collagen cross-linking has been calculated. spectra DPSCs after 21 days...
The present study compared two pH-cycling models designed to induce subsurface lesions (SLs) with a less demineralized surface layer on teeth, the aim of developing new technologies for assessment such by examining performance confocal Raman microscopy detection white spot (WSLs). Twelve sound premolars were exposed sets model conditions (A, B) SLs. Teeth which had formed in vivo used as positive controls. All specimens inspected using an intraoral camera and detect small changes appearance...