A5010902070- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
- Advanced Biosensing Techniques and Applications
- Ultrasound Imaging and Elastography
- 3D Printing in Biomedical Research
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cellular Mechanics and Interactions
- Image Processing Techniques and Applications
- Force Microscopy Techniques and Applications
- Advanced Optical Sensing Technologies
- Coronary Interventions and Diagnostics
- Cerebrovascular and Carotid Artery Diseases
- Cell Adhesion Molecules Research
- Nanofabrication and Lithography Techniques
- bioluminescence and chemiluminescence research
- Optical Coatings and Gratings
- Advanced optical system design
- Optical Imaging and Spectroscopy Techniques
- Collagen: Extraction and Characterization
- Near-Field Optical Microscopy
- Advanced biosensing and bioanalysis techniques
- Optical measurement and interference techniques
Laboratoire des Sciences de l'Ingénieur, de l'Informatique et de l'Imagerie
2023-2025
Imperial College London
2015-2024
École Polytechnique
2020-2023
Centre National de la Recherche Scientifique
2020-2021
Inserm
2020-2021
Université Paris-Saclay
2020
How cancer cells determine their shape in response to three-dimensional (3D) geometric and mechanical cues is unclear. We develop an approach quantify the 3D cell of over 60,000 melanoma collagen hydrogels using high-throughput stage-scanning oblique plane microscopy (ssOPM). identify stereotypic environmentally dependent changes protrusivity depending on whether a proximal flat rigid surface or embedded soft environment. Environmental sensitivity metrics calculated for small molecules gene...
Abstract Light sheet fluorescence microscopy has previously been demonstrated on a commercially available inverted microscope frame using the method of oblique plane (OPM). In this paper, OPM is adapted to allow time-lapse 3-D imaging biological cultures in glass-bottomed 96-well plates stage-scanning approach (ssOPM). Time-lapse multicellular spheroids expressing glucose Förster resonance energy transfer (FRET) biosensor 16 fields view with image acquisition at 10 minute intervals. As...
Oblique plane microscopy (OPM) is a form of light sheet that uses single high numerical aperture microscope objective for both fluorescence excitation and collection. In this paper, measurements the relative collection efficiency OPM are presented. An system incorporating two sCMOS cameras then introduced enables isolated cardiac myocytes to be studied continuously 22 seconds in dimensions at 667 frames per second with 960 × 200 pixels 30 20 voxels 25 volumes second. cases able record...
Improving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast in vivo . However, photoperturbation remains poorly investigated. We show here that heart beat rate zebrafish embryos a sensitive probe linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency wavelength, we derive guidelines find best balance between signal...
Over the past few decades, a multitude of optical imaging techniques have emerged. Among them, full-field coherence tomography (FF-OCT) has gained significant importance in various biomedical applications. Indeed, FF-OCT stands out as noninvasive and label-free method capable generating high-resolution 3D microscopic images light-scattering biological specimens. However, approach is limited for in-vivo from lack specificity required accurate diagnosis. Hence, there need to access incorporate...
Imaging a large number of samples is necessary to improve statistical robustness in biological assays. Using standard multiwell plates not possible usual light-sheet microscopes. One solution use an Oblique Plane Microscope (OPM), which special fluorescence light sheet microscope, with single objective near the sample. To avoid aberrations, OPM generally uses water or silicon immersion match refractive index In this work we present oil-immersed and experimentally demonstrate possibility...
Light-sheet fluorescence microscopy is a method of choice for multiscale live imaging. Indeed, its orthogonal geometry results in high acquisition speed, large field-of-view and low photodamage. Its combination with multiphoton excited improves imaging depth biological tissues. However, it appears femtosecond laser sources commonly used at an 80 MHz repetition rate may not be optimized to take full advantage light-sheet illumination during Hence, we investigated the nature induced...
Abstract In order to invade 3D tissues, cancer cells dynamically change cell morphology in response geometric and mechanical cues the environment. But how determine their shape versus 2D environments is poorly understood. Studying single determination has historically been technically difficult due lack of methodologies directly compare two environments. We developed an approach study by measuring at different depths collagen using stage-scanning oblique plane microscopy (ssOPM). find...
Oblique plane microscopy (OPM) is a light-sheet fluorescence technique that implemented on standard inverted microscope frame. OPM uses single high numerical aperture objective to both produce tilted excitation and image the emitted from back cameras. It therefore compatible with conventional sample-mounting techniques such as slides multiwell plates. Four laser lines two high-speed sCMOS cameras separate emission filters enable simultaneous imaging of several fluorophores spectral...
Light sheet fluorescence microscopy (LSFM) enables fast 3D imaging of live cells, however the traditional LSFM geometry is not compatible with conventional multiwell plates used in high content microscopy. We have developed an automated platereader based on oblique plane microscope (OPM) that plates. The system studies cells cultured a collagen matrix. Cells across 30 different conditions are imaged every 5 minutes for 12 hours. A custom segmentation and tracking pipeline analyses cell...
We report on recent advances in multiphoton light-sheet microscopy to perform fast multimodal imaging combining fluorescence with second-harmonic generation and mitigate photodamage during vivo of embryos.
Abstract Improving the imaging speed of multiphoton microscopy is an active research field. Among recent strategies, light-sheet illumination holds distinctive advantages for achieving fast in vivo . However, photoperturbation remains poorly investigated. We show here that heart beat rate zebrafish embryos a sensitive probe linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency wavelength, we derive guidelines balance signal...
Light-sheet illumination improves multiphoton imaging speed. By optimizing laser pulse frequency using the quantification of photodamage in embryos, we demonstrate an order-of-magnitude signal increase live two-photon light-sheet imaging.
Light-sheet illumination enables major increase in multiphoton imaging speed for vivo studies. However, photoperturbation light-sheet microscopy remains poorly investigated. We show here that the heart beat rate of zebrafish embryos is a sensitive probe linear and nonlinear photoperturbations. By analyzing its behavior with respect to laser power, pulse frequency wavelength, we derive guidelines balance signal photoperturbation. then demonstrate one order-of-magnitude enhancement over...